Thermo Scientific Vanquish VH-D10 Operating Manual page 141

Ending the flushing procedure
Diode Array Detectors (VH-D10)
Operating Manual
4. Monitor the operating pressure:
Situation Reason
If the pressure The clogging was not
remains high at a removed.
value similar to the
value that you wrote
down
If the pressure is The clogging is removed
decreasing to a from the flow cell. As the
normal state and only blockage is flushed out, the
a little liquid flows pressure at the pump is
through the usually decreasing. More of
overpressure line. the liquid will flow through
the flow cell and less fluid is
leaving the overpressure
line.
1. Stop the pump flow.
2. Disconnect the back-flush components:
a) Disconnect the flow cell capillary and the back-flush waste line
(no. 2) from the flow cell.
b) Disconnect the inlet line (no. 1a) from the pump.
c) Remove the overpressure line (no. 1c) and back-flush waste line
(no. 2) from the waste.
3. Install the protection caps to the open Viper fittings of the flow cell
capillary (no. 1e) and of the back-flush waste line (no. 2).
4. Pack and store the components of the kit in their original packaging.
5. Install the flow cell to the detector (see
(} page 130)).
Make sure that the first solvent that is delivered to the flow cell is
miscible with the solvent that was used as back-flushing agent.
7 • Maintenance and Service
Steps
Thermo Fisher Scientific
recommends performing
another backward and
forward flushing cycle:
1. Stop the pump flow.
2. Follow the steps starting
with the Back-flushing
section.
If repeated flushing did not
remove the clogging,
proceed with the Ending the
flushing procedure section
below.
Proceed with the next steps.
Installing the Flow Cell
Page 141