Thermo Scientific Vanquish VH-D10 Operating Manual page 127

Follow these steps
Diode Array Detectors (VH-D10)
Operating Manual
3. Make sure that residual sample components, impurities from the
column, aggressive solvents or eluates with salt contents are
completely flushed out of the flow cell.
4. If the UV lamp is turned on
Close the shutter in the light path before the flow cell.
‑or‑
Turn off the UV lamp.
5. Stop the pump flow to the flow cell.
1. Unpack the components from the flushing and injection kit. Remove
the protection cap from the Viper fitting. Keep the packaging.
2. Screw the threaded end of the flushing adapter to the syringe.
3. Draw up highly pure isopropanol with the syringe through the Viper
adapter, for example 1.0 mL.
4. Disconnect the capillary from the flow cell inlet.
5. Connect the Viper adapter to the flow cell inlet.
6. Push the isopropanol in the syringe into the flow cell. Make sure that
no residual air in the syringe is pushed into the flow cell.
7. Disconnect the waste line from the flow cell outlet.
8. Disconnect the flushing adapter from the flow cell inlet.
9. Install the flow cell plugs to the flow cell inlet and flow cell outlet.
10. Unscrew the flushing adapter from the syringe.
11. Make sure that the adapter and the syringe are empty and dry.
12. Put the protection cap onto the Viper adapter.
13. Pack and store the components of the kit in their original packaging.
14. If the flow cell is to be stored in its packaging
Remove the flow cell from the device and store it (see
Flow Cell (} page 129)).
7 • Maintenance and Service
Removing the
Page 127