Thermo Scientific Vanquish VH-D10 Operating Manual page 128

Diode array detectors
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7
Maintenance and Service
7.7.1.1
Performing a Manual Injection
Parts required
Page 128
A sample can also be injected manually using the flushing and injection
kit.
Flushing and injection kit for flow cells, including a syringe and a Viper
adapter
NOTICE
Particulate matter from the eluate can deposit in the flow cell and clog
it.
Be careful not to inject any particulate matter into the flow cell.
Only experienced users should perform a manual injection.
The manual injection requires setting suitable detection parameters,
starting acquisition, performing an autozero and data interpretation
afterward. The related injection procedures of autozero injection,
sample injection and flushing are similar to the flushing procedure
described in
Preparing the Flow Cell for Storage
Observe the following guidelines when performing a manual injection:
Avoid injecting any particulate matter into the flow cell.
All solvents used must be miscible.
Flush the sample completely out of the flow cell after measurement.
(} page 126).
Diode Array Detectors (VH-D10)
Operating Manual

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