Flow Cell Specifications - Thermo Scientific Vanquish VH-D10 Operating Manual

9 • Specifications
Temperature reference conditions for noise, drift and linearity specifications
Time constant and response time
9.1.2

Flow Cell Specifications

Specification
Path length
Illuminated volume
Dispersion volume
Pressure limit
Max. eluate temperature limit
Materials in the flow path
Biocompatibility
Normal-phase compatibility
Page 174
For drift tests, ASTM requires that temperature changes stay below 2 °C/
hour over a period of one hour. The drift specification above is based on
these conditions. Larger changes in the ambient temperature will result
in a larger drift.
For best performance, minimize the frequency and the amplitude of
changes in the ambient temperature to 1 °C/hour.
All performance tests should be done with a completely warmed up
optical unit (lamp turned on for more than two hours). ASTM requires
that the detector should be turned on for at least 24 hours before the
testing is started.
TIP Flow cells may show an increased drift for several hours after
storage, contamination, or if the flow was stopped for some time.
Consider this when testing the drift specification of the device.
According to ASTM E1657-98, the time constant is converted to the
response time by multiplying by the factor 2.2 (i.e., response time =
2.2 x time constant).
The flow cell performances are specified as follows:
LightPipe flow cell, standard,
10 mm
10 mm
2 µL
0.8 µL
6 MPa
50 °C
Fused silica, PEEK, perfluoro-
elastomer, titanium
Yes
No
LightPipe flow cell, high
sensitivity, 60 mm
60 mm
13 µL
4.0 µL
6 MPa
50 °C
Fused silica, PEEK, perfluoro-
elastomer, titanium
Yes
No
Diode Array Detectors (VH-D10)
Operating Manual