Observation Methods; Transmitted Light Brightfield Observation Procedures - Olympus MX63 Instructions Manual

5

Observation methods

The dummy slider in the DIC prism insertion slot is effective for preventing flare in observations other than the DIC
observation. It is, therefore, recommended to leave it mounted in any observation method.
Setting the DF light path prevents the half mirror from entering the transmitted light illumination light path.
TIP
Therefore, the transmitted light can be used most effectively.

5-1 Transmitted light brightfield observation procedures

Set the main switch of the microscope frame
1
to (ON) and set the light intensity knob
to the low voltage position.
Set the observation light path selection knob
2
to the DF (reflected light darkfield) position.
5 Set the main switch of the light source to (ON).
6 Place a sample for observation on the stage.
Engage the 5X or 10X objective in the light path
7
using the objective selection button and
bring the sample into focus.
Engage the objective with the magnificati
11
on to be used in the light path and
bring the sample into focus.
Observation
44 44
(Can be set to the BF position if there is sufficient brightness)
[Trinocular tube only]
3
Push in the light path selection knob.
Remove the analyzer and the
4
polarizer from the light path.
Adjust the brightness.
8
Adjust the interpupillary distance.
9
Adjust the diopter.
10
Adjust the brightness.
12
Insert the required filters.
13
Adjust the field diaphragm and
14
aperture diaphragm.
Operation part
Main switch
Light intensity knob
Observation light path selection knob
Light path selection knob
Main switch
X-axis/Y-axis knobs
Objective selection button
Coarse focusing knob / Fine focusing
knob
Light intensity volume knob
Interpupillary distance adjustment
scale
Diopter adjustment ring
Objective selection button
Light intensity volume knob
Filter insertion slot
Field diaphragm lever (MX-TILLB)
Condenser height adjustment ring
(MX-TILLB)
Aperture diaphragm lever or aperture
diaphragm ring
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