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4
Various observation methods
This section describes the operating procedures of the observations other than the reflected brightfield observation. The
operating procedures of the reflected brightfield observation are described in "3 Basic operations of the microscope
(brightfield observation)".
4-1 Reflected light darkfield observation procedures
1
Turn ON the main switch.
2
Select the darkfield (DF) observation.
3
Select the light path. (Trinocular tube only)
4
Place a sample on the stand.
5
Engage the 10X objective in the light path.
6
Bring the sample into focus.
10
Open the aperture diaphragm, and
adjust the field diaphragm.
11
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
36
Remove the analyzer, polarizer or
filters from the light path.
7
Adjust the brightness.
8
Adjust the interpupillary distance.
9
Adjust the diopter.
12
Adjust the brightness.
Start observation.
Main switch of power supply
Observation method selector knob of the
reflected light illuminator
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Coarse / Fine focusing knob of the BXFM
frame
Brightness control knob of the power supply
Binocular tube
Diopter adjustment ring
Aperture diaphragm knob of the reflected
light illuminator
Field diaphragm knob of the reflected light
illuminator
Nosepiece
Coarse / Fine focusing knob of the BXFM
frame
Brightness control knob of the power supply
(page 16)
(page 16)
(page 18)
(page 18)
(page 18)
(page 19)
(page 20)
(page 21)
(page 23)
(page 23)
(page 30)
(page 27)
(page 19)
(page 20)
(page 21)
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