Olympus BX51 Instructions Manual
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Olympus BX51 Instructions Manual

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INSTRUCTIONS
BX51
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure
the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro-
scope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 6 5 5
This publication is printed on 100% recycled paper

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  • Page 1 BX51 SYSTEM MICROSCOPE This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro- scope, we recommend that you study this manual thoroughly before operating the microscope.

  • Page 3: Table Of Contents

    BX51 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 7, “ASSEMBLY” (pages 28 to 30) carefully. IMPORTANT — Be sure to read this section for safe use of the equipment. —...
  • Page 5 UIS2/UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.
  • Page 6 4. The BX51 series can be used with up to two intermediate attachments (e.g. a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.). For restrictions when using two intermediate attachments, make sure to read the instruction manual provided with the respective intermediate attach- ments.

  • Page 7: Maintenance And Storage

    BX51 Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.
  • Page 8 NOMENCLATURE } If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 28 to 30). Transmitted light specification model BX51TF or microscope frame Light path selector knob (Page 16) Interpupillary distance adjustment scale (Page 15) Condenser height adjustment knob (Page 18) Allen screwdriver (accommodation position)
  • Page 9 BX51 Transmitted light/reflected light specification model BX51TRF microscope frame The controls are identical to those of the transmitted light specification models except for the transmitted/ NOTE reflected light switch and the reflected light system (vertical illuminator, reflected lamp housing). Reflected lamp housing* Reflected light illuminator* ·...

  • Page 10: Transmitted Light Brightfield Observation Procedure

    TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE (Controls Used) (Page) @Main switch Set the main switch to “ I ” (ON) and adjust the brightness. (P. 8) ²Brightness adjustment knob ³Transmitted/reflected light (P. 5) Set the transmitted light switch (BX51TRF only) Engage only the LBD filter. |LBD filter knob (P.
  • Page 11 BX51 ‰ ƒ Œ ‹ ³ ‡ … š ™ † Š › ² Š œ } Copy the observation procedure pages on separate sheets and post it near your microscope.

  • Page 12: Using The Controls

    USING THE CONTROLS 3-1 Base Voltage Indication (Fig. 3) 1. Turn the brightness adjustment knob @ clockwise to increase the volt- age and make illumination brighter. 2. The numerals to the right of the lamp voltage indicator LEDs ² indicate the voltage.
  • Page 13 ³ LBD (for color balancing, daylight filter) Fig. 5 OP (optional) filter set* * Ask your Olympus representative to mount the filters. Mounting a Single Filter (Fig. 6) ƒ A filter with a diameter of 45 mm ƒ can be placed on the filter mount on the base.
  • Page 14 Mounting the Filter Cassette 1. Fully loosen the filter cassette clamping screw †. (Fig. 8) ‡ 2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot Š on the filter mount, then snap the filter cassette into †...

  • Page 15: Focusing Block

    BX51 3-2 Focusing Block Replacing the Fine Adjustment Knob (Fig. 11) # The fine adjustment knob has been attached on the right side at the factory. }The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or X-/Y-axis knobs.

  • Page 16: Stage

    3-3 Stage Placing the Specimen ² # The dimensions of the slide glass should be 26 x 76 mm with thick- ness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. # When observing very large specimens, remove the slide holder and place the specimen directly on the stage.
  • Page 17 BX51 Adjusting the X- and Y-Axis Knob Tension (Fig. 17) 1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the adjustment knobs. ² 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clock- wise (in the direction of the arrow) increases the tension and counter- clockwise decreases it.

  • Page 18: Rotating The Stage

    Rotating the Stage (Fig. 18) 1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. # A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunc- tion.

  • Page 19: Observation Tube

    BX51 3-4 Observation Tube Adjusting the Interpupillar Distance (Fig. 21) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated.

  • Page 20: Using The Eyepiece Micrometer Disk

    100% for binocu- 100% for TV/ lar eyepieces photography 50% for binocular Fig. 26 eyepieces, 50% for U-TR30NIR * TV/photography * With the infrared trinocular tube, infrared observation up to 1000 nm is possible. For details, consult your Olympus representative.

  • Page 21: Adjusting The Tilt

    (by 45 mm). The U-ETBI is the erect image model and the U-TTBI is the inverted image model, and both models are of the same size. # The intermediate attachments that can be combined with the U-TTBI are limited. For details, please contact Olympus. Fig. 28...

  • Page 22: Condenser

    3-5 Condenser Centering the Condenser (Figs. 29 & 30) 1. Turn the condenser height adjustment knob @ to raise the condenser to its upper limit. 2. Focus on the specimen using the 10X objective. # When using the U-SC3 swing-out condenser, move the top lens into ³...
  • Page 23 BX51 Aperture Iris Diaphragm (Figs. 31 & 32) Aperture iris 70-80% diaphragm image · The aperture iris diaphragm determines the numerical aperture of the 30-20% illumination system. It has an effect of adjusting image resolution and contrast. Stopping down the aperture iris diaphragm increases the depth of focus.

  • Page 24: Immersion Objectives

    3-6 Immersion Objectives # Be sure to use the provided Olympus Immersion oil. Using Immersion Objectives (Fig. 33) 1. Focus on the specimen with objectives in the order of lower-power to higher-power ones. 2. Before engaging the immersion objective, place a drop of provided immersion oil onto the specimen at the area to be observed.

  • Page 25: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem...
  • Page 26 Problem Cause Remedy Page Immersion oil contains bubbles. Remove the bubbles. e) Visibility is poor. · Image is not poor. Recommended immersion oil is not Use the provided immersion oil. · Contrast is poor. used. · Details are indistinct. Dirt/dust on specimen. Clean it.
  • Page 27 BX51 Problem Cause Remedy Page c) Image cannot be focused. When adjusting stage height, you forgot Reattach upper stopper screw. to reattach upper stopper screw. d) Coarse adjustment will not go all Pre-focusing lever is locked at a too low Unlock pre-focusing lever.

  • Page 28: Specifications

    SPECIFICATIONS Item Specification 1. Optical system UIS2/UIS (Universal Infinity System) optical system (featuring infinity correction) 2. Illumination Built-in transmitted Koehler illumination 12V, 100W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 5761) or 12 V, 50 W long-life halogen bulb (pre-centered) 12V50WHAL-L (LIFE JC) (Average life time: Approximately 2,000 hr.
  • Page 29 BX51 Item Specification 7. Condenser U-AAC Type U-AC2 U-SC3 Abbe Swing-out Achromat/ Aplanat N.A. 1.10 0.9 - 0.1 1.40 Aperture iris dia- With numerical aperture scale phragm Objective range 4X (for FN 22 widefield), 1.25X (for FN 22 10X - 100X (for FN 26.5...

  • Page 30: Optical Characteristics

    Iris: Iris diaphragm length NOTE Field number (FN) Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces Color band Cover glass thickness and objectives that can be combined with this microscope.
  • Page 31 BX51 Eyepiece Optical Cover Reso- glass WHN10X (FN22) character Magnifi- W.D. N.A. thick- lution Remark Depth of cation (mm) Field of ness (µm) focus Total mag. view Objective (mm) (µm) UIS2 MPLFLN Series Plan Semi 0.80 0.42 500X 0.44 Apochromat (FN26.5)

  • Page 32: (Uis2/Uis Series)

    However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or remove this module).
  • Page 33 BX51 7-2 Detailed Assembly Procedures Installing the Bulb (Figs. 35 - 37) }Use only the designated bulb 12V100WHAL-L (PHILIPS 7724) or 12V50WHAL-L (LIFE JC). 1. Fully loosen the lamp housing clamping screw @ on to of the lamp housing cover with the provided Allen screwdriver.

  • Page 34: Proper Selection Of The Power Supply Cord

    Always use the power cord provided by Olympus. If no power cord is provided with the microscope, please select the proper power cord by referring to section “ PROPER SELECTION OF THE POWER SUPPLY CORD ”...

  • Page 35: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 36 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
  • Page 37 MEMO...
  • Page 38 MEMO...
  • Page 40 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Wendenstrasse 14-18, 20097 Hamburg, Germany 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. One Corporate Drive, Orangeburg, NY 10962, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London, EC1Y OTX, U.K.

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