Transmitted Brightfield/Darkfield Observation Methods - Olympus CX43 Instructions Manual

3

Transmitted brightfield/darkfield observation methods

This section describes the procedures of the transmitted brightfield/darkfield observation, which is the basic of all observations.
The transmitted phase contrast observation and transmitted simple polarization observation are explained in "5 Various observation
methods" on page 23.
If the analyzer for simple polarization and the polarizer are engaged in the light path, remove them from the light path.
Set the main switch to (ON).
Select BF (brightfield observation).
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen into focus and adjust the brightness.
Engage the objective you use in the light path and bring
the specimen into focus.
For brightfield observation, adjust the aperture diaphragm.
For darkfield observation, select DF (darkfield observation).
Observation
Adjust the interpupillary
distance.
Adjust the diopter.
Place the necessary filter.
Adjust the brightness.
(Operation portion)
1
Main switch
2
Condenser turret
3
Specimen holder
4
X-axis/Y-axis knobs
5
Revolving nosepiece
6
Coarse/fine focusing knobs
7
Brightness adjustment knob
8
Binocular portion
9
Diopter adjustment ring
5
Revolving nosepiece
6
Coarse/fine focusing knobs
a
Aperture diaphragm lever
2
Condenser turret
b
Various filters
7
Brightness adjustment knob
CX43
(Page)
9
19
10
11
14
14
9
16
17
14
14
20
19
9
9
7