Using The Oil Immersion Objective - Olympus MX63 Instructions Manual

3-14 Using the oil immersion objective

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Apply the specified oil (immersion oil) to the tip of the oil immersion objective.
Otherwise, the observation image can not be brought into focus.
Always use the immersion oil made by Olympus. If you
NOTE
use the immersion oil other than those made by Olympus,
the correct optical performance cannot be exhibited.
1
Apply a drop of immersion oil on the sample.
2
Engage the oil immersion objective.
3
Rotate the coarse / fine focusing knobs to bring the sample into focus.
· If bubbles are contained in the oil, the visibility of the image
NOTE
is deteriorated. Check that the bubbles are not contained
in the immersion oil.
· To check the presence of bubbles, remove the eyepiece,
open the field diaphragm and the aperture diaphragm
completely and look at the objective pupil in the observation
tube.
· Remove bubbles by moving the sample or the stage up/
down/right/left. Depending on the sample, the immersion
oil may be adhered to the sample and bubbles may not be
removed easily.
4
After use, lower the stage and rotate the revolving nosepiece, and
remove the objective attached with oil from the specimen.
5
Wipe off the immersion oil thoroughly from the tip of the objective and
the tip of the condenser lens with the cleaning paper or the gauze
slightly moistened with absolute alcohol. Wipe off the immersion oil
from the specimen in the same procedures.
If lenses or samples are left keeping the immersion oil
NOTE
attached, the immersion oil adheres and the correct
observation may not be performed.
Follow the cautions indicated in the label of the immersion
CAUTION
oil.