Olympus BX51 Instructions Manual
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Olympus BX51 Instructions Manual

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INSTRUCTIONS
BX51/BX52
SYSTEM MICROSCOPE
This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure
the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro-
scope, we recommend that you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 9 8 5 5

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Summary of Contents for Olympus BX51

  • Page 1 BX51/BX52 SYSTEM MICROSCOPE This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro- scope, we recommend that you study this manual thoroughly before operating the microscope.

  • Page 2: Table Of Contents

    BX51/52 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 7, “ASSEMBLY” (pages 27 to 29) carefully. IMPORTANT — Be sure to read this section for safe use of the equipment. —...

  • Page 3: Safety Precautions

    UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.
  • Page 4 4. The BX51/52 series can be used with up to two intermediate attach- ments (e.g. a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.). For restrictions when using two intermediate attachments, make sure to read the instruction manual provided with the respective intermediate attachments.

  • Page 5: Maintenance And Storage

    BX51/BX52 Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these chemicals away from open flames or potential sources of electrical sparks ––...

  • Page 6: Nomenclature

    NOMENCLATURE } If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 27 to 29). Transmitted light specification models BX51TF or BX52TF microscope frame. Light path selector knob (Page 16) Interpupillary distance adjustment scale (Page 15) Condenser height adjustment knob (Page 18) Allen screwdriver (accommodation position)
  • Page 7 BX51/BX52 Transmitted light/reflected light specification models BX51TRF and BX52TRF microscope frames The controls are identical to those of the transmitted light specification models except for the transmitted/ NOTE reflected light switch and the reflected light system (vertical illuminator, reflected lamp housing).

  • Page 8: Transmitted Light Brightfield Observation Procedure

    TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE (Controls Used) (Page) Set the main switch to “ I ” (ON) and adjust the @Main switch brightness. (P. 8) ²Brightness adjustment knob ³Transmitted/reflected light (P. 5) Set the transmitted light switch (BX51TRF/BX52TRF only) Engage only the LBD filter. |LBD filter knob (P.
  • Page 9 BX51/BX52 ‰ ƒ Œ ‹ ³ ‡ … š ™ † Š › ² Š œ } Copy the observation procedure pages on separate sheets and post it near your microscope.

  • Page 10: Using The Controls

    USING THE CONTROLS 3-1 Base Voltage Indication (Fig. 3) 1. Turn the brightness adjustment knob @ clockwise to increase the volt- age and make illumination brighter. 2. The numerals to the right of the lamp voltage indicator LEDs ² indicate the voltage.
  • Page 11 ³ LBD (for color balancing, daylight filter) Fig. 5 OP (optional) filter set* * The set can be ordered at your local Olympus representative. Mounting a Single Filter (Fig. 6) ƒ A filter with a diameter of 45 mm ƒ can be placed on the filter mount on the base.
  • Page 12 Mounting the Filter Cassette 1. Fully loosen the filter cassette clamping screw †. (Fig. 8) ‡ 2. Align the key ‡ on the bottom surface of the filter cassette with the positioning slot Š on the filter mount, then snap the filter cassette into †...

  • Page 13: Focusing Block

    BX51/BX52 3-2 Focusing Block Replacing the Fine Adjustment Knob (Fig. 11) }The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or X-/Y-axis knobs. Usually attach the knob on the opposite side to the X- and Y-axis knobs.

  • Page 14: Stage

    3-3 Stage Placing the Specimen ² # The dimensions of the slide glass should be 26 x 76 mm with thick- ness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm. # When observing very large specimens, remove the slide holder and place the specimen directly on the stage.
  • Page 15 BX51/BX52 Adjusting the X- and Y-Axis Knob Tension (Fig. 17) 1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the adjustment knobs. ² 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clock- wise (in the direction of the arrow) increases the tension and counter- clockwise decreases it.
  • Page 16 Rotating the Stage (Fig. 18) 1. Slightly loosen the stage clamping screw @. 2. The stage can be rotated both clockwise and counterclockwise by the stage clamping screw. # A click may be heard and felt during rotation. However, this is due to the construction of the substage and does not indicate a malfunc- tion.

  • Page 17: Observation Tube

    BX51/BX52 3-4 Observation Tube Adjusting the Interpupillar Distance (Fig. 21) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated.

  • Page 18: Selecting The Light Path

    Using Eyepiece Micrometer Disks (Fig. 25) Eyepiece micrometer disks can be inserted into WH10X-H (or WH10X) eyepieces. Use 24 mm diam. x 1.5 mm thick micrometer disks. Following Fig. 25, unscrew the micrometer mounting frame ² from the ² eyepiece and place a micrometer disk @ into the mounting frame. The engraving on the micrometer disk should face downward into the mi- crometer mounting frame.
  • Page 19 BX51/BX52 Adjusting the Tilt (with the U-TBI3) (Fig. 27) }Adjust the height and tilt of the observation tube to obtain the most com- fortable viewing position. Holding the binocular section with both hands, raise or lower it to the desired position.

  • Page 20: Condenser

    3-5 Condenser Centering the Condenser (Figs. 29 & 30) 1. Turn the condenser height adjustment knob @ to raise the condenser to its upper limit. 2. Focus on the specimen using the 10X objective. # When using the U-SC3 swing-out condenser, move the top lens into ³...

  • Page 21: Condenser

    BX51/BX52 Aperture Iris Diaphragm (Figs. 31 & 32) Aperture iris 70-80% diaphragm image · The aperture iris diaphragm determines the numerical aperture of the 30-20% illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and also increases the depth of focus.

  • Page 22: Immersion Objectives

    3-6 Immersion Objectives Using Immersion Objectives (Fig. 33) 1. Focus on the specimen with a lower-power objective. 2. Place a drop of provided immersion oil onto the specimen at the area to be observed. 3. Turn the revolving nosepiece to engage the immersion objective, then focus using the fine adjustment knob.

  • Page 23: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem...
  • Page 24 Problem Cause Remedy Page Immersion oil contains bubbles. Remove the bubbles. e) Visibility is poor. · Image is not poor. Recommended immersion oil is not Use the provided immersion oil. · Contrast is poor. used. · Details are indistinct. Dirt/dust on specimen. Clean it.
  • Page 25 BX51/BX52 Problem Cause Remedy Page c) Image cannot be focused. When adjusting stage height, you forgot Reattach upper stopper screw. – – to reattach upper stopper screw. d) Coarse adjustment will not go all Pre-focusing lever is locked at a too low Unlock pre-focusing lever.

  • Page 26: Specifications

    SPECIFICATIONS Item Specification 1. Optical system UIS (Universal Infinity System) optical system 2. Illumination Built-in transmitted Koehler illumination 12V, 100W long-life halogen bulb (pre-centered) 12V100WHAL-L (PHILIPS 5761) or 12 V, 50 W long-life halogen bulb (pre-centered) 12V50WHAL-L (LIFE JC) (Average life time: Approximately 2,000 hr. when used as directed) Light intensity voltage range: 1.0 to 12.0 V DC (continuous) Light intensity preset button (voltage adjustment range: 1.0 to 12.0 V DC) Rated voltage: 100-120/220-240 V...
  • Page 27 BX51/BX52 Item Specification 7. Condenser U-AAC Type U-AC2 U-SC3 Abbe Swing-out Achromat/ Aplanat N.A. 1.10 0.9 - 0.1 1.40 Aperture iris dia- With numerical aperture scale phragm Objective range 4X (for FN 22 widefield), 1.25X (for FN 22 10X - 100X (for FN 26.5...

  • Page 28: Optical Characteristics

    The figure on the right shows the performance data engraved on the objectives. Numerical aperture NOTE Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces Cover glass thickness Mechanical tube length and objectives that can be combined with this microscope.

  • Page 29: Assembly

    However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench ( ) for clamping the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble or remove this module).

  • Page 30: Attaching Condenser

    7-2 Detailed Assembly Procedures Installing the Bulb (Figs. 35 - 37) }Use only the designated bulb 12V100WHAL-L (PHILIPS 7724) or 12V50WHAL-L (LIFE JC). 1. Fully loosen the lamp housing clamping screw @ on to of the lamp housing cover with the provided Allen screwdriver. ²...
  • Page 31 ” (OFF) before con- ³ necting the power cord. Always use the power cord provided by Olympus. IF no power cord is provided with the microscope, please select the proper power cord by referring to section “PROPER SELECTION OF THE POWER ²...

  • Page 32: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the eqipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the eqipment.
  • Page 33 BX51/BX52 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir-...
  • Page 34 MEMO...
  • Page 35 MEMO...
  • Page 36 MEMO...
  • Page 37 2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia This publication is printed on recycled paper.

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Bx52

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