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5-4 Reflected light fluorescence observation procedures
For this observation, the reflected light fluorescent mirror unit must be attached to the OP position of the observation light
path by Olympus.
Attach the mercury lamp housing and perform the centering of the burner before observation. Set the AS PRESET switch
of the objective in use to "1" with which the aperture diaphragm is fully opened. For details, see "Table 1 Recommended
AS PRESET values" (P.100).
When using the mercury lamp housing, the difference in light intensity between the fluorescence and
CAUTION
brightfield observations is increased.
To compensate this difference, insert the ND filter (26ND0.5) provided with the adapter (MX-HGAD) in
the brightfield observation light path. (For attaching the mirror unit, contact Olympus.)
If the observation light is too bright, insert the ND filter to reduce the light.
NOTE
Set the main switch to (ON).
Select OP (optional mirror unit).
[Trinocular tube only]
Push in the light path selection knob.
Place a sample for observation on the stage.
Engage the 5X or 10X objective in the light path
using the objective selection button and
bring the sample into focus.
Open the aperture diaphragm.
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Observation
Adjust the brightness
(illumination voltage).
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the brightness.
Insert the required filters.
MX63/MX63L
Operation part
Main switch
Observation light path selection knob
Light path selection knob
X-axis/Y-axis knobs
Objective selection button
Coarse focusing knob / Fine focusing
knob
Light intensity knob
Interpupillary distance adjustment
scale
Diopter adjustment ring
Aperture diaphragm open / close
button
Objective selection button
Coarse focusing knob / Fine focusing
knob
Light intensity knob
Filter insertion slot
Page
P.24
P.25
P.26
P.27
P.28
P.29
P.32
P.35
P.35
P.38
P.28
P.29
P.32
P.39
55
55
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