Reflected Light Differential Interference Contrast (Dic) Observation Procedures - Olympus MX63 Instructions Manual

5-2 Reflected light differential interference contrast (DIC) observation procedures

For this observation, the DIC mirror unit (U-MDIC3 or U-MDICAF3) must be attached to the OP position of the observation
light path by Olympus.
U-MDIC3: The analyzer and the polarizer are built-in.
U-MDICAF3: As only the polarizer is built-in, the analyzer (U-AN360-3) must be inserted to adjust the crossed nicols.
When using the mercury lamp housing, be sure to use the U-25L42 filter to prevent the polarizer from
NOTE
tarnish.
Set the main switch to (ON).
Push in the light path selection knob to the OP position
and select the DIC observation light path.
Place a sample for observation on the stage.
Engage the 10X or 20X objective in the light path
using the objective selection button and
bring the sample into focus.
Adjust the aperture diaphragm.
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Adjust the prism of the DIC slider.
Observation
50 50
[Trinocular tube only]
Push in the light path selection knob.
Set the analyzer.
Set the DIC slider.
Adjust the brightness
(illumination voltage).
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the brightness.
Operation part
Main switch
Observation light path selection knob
Light path selection knob
X-axis/Y-axis knobs
Objective selection button
Coarse focusing knob / Fine focusing
knob
Analyzer insertion slot
DIC slider insertion slot
Light intensity knob
Interpupillary distance adjustment
scale
Diopter adjustment ring
Aperture diaphragm open/close
button
Objective selection button
Coarse focusing knob / Fine focusing
knob
Light intensity knob
DIC slider
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