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48
48
Using the aperture diaphragm for transmitted
4
light illumination
In general, adjusting the aperture diaphragm to 70% to 80% of the
numerical aperture of the objective allows you to obtain an image with
good contrast. However, the NA may become insufficient when using the
objectives with NA0.6 (MX-TILLB) or NA0.5 (MX-TILLA) or higher.
a
1
2
3
b
TIP
4
Reflected light observation of the sample
· When observing a transparent sample, such as a glass substrate, etc.
using the 20X or lower objective, the annular flare may be observed. In this
case, rotate the aperture diaphragm lever b (or aperture diaphragm ring)
in arrow direction to narrow down to the minimum to eliminate the flares.
· When not using the transmitted light:
MX-TILLB: Narrow down the aperture diaphragm to minimum.
MX-TILLA: Push in the anti-reflection shutter.
Set the observation light path selection knob a to the DF position.
Rotate the revolving nosepiece to engage the 10X objective in the
light path and bring the sample into focus.
Pull out the eyepiece. While looking into the eyepiece sleeve, rotate the
aperture diaphragm lever b toward right side. (At this time, narrowing
down the aperture diaphragm to 70% to 80% allows you to obtain the
image with good contrast.)
70 %
30 %
Aperture diaphragm
image
Depending on the sample you observe, the contrast may
be improved by narrowing down the aperture diaphragm
furthermore. However, if the aperture diaphragm is narrowed
down excessively with the low magnification objective, the
illumination unevenness may occur in observed images or
photographs.
Attach the eyepiece to the original position.
Pupil of objective
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