Olympus BX46 Instructions Manual
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Olympus BX46 Instructions Manual

Clinical microscope
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INSTRUCTIONS
BX46
CLINICAL MICROSCOPE
This instruction manual is for the Olympus Clinical Microscope Model BX46.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 5 3

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  • Page 1 BX46 CLINICAL MICROSCOPE This instruction manual is for the Olympus Clinical Microscope Model BX46. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope.
  • Page 2 Refer to your local Olympus distributor in EU for return and/or collection systems available in your country. NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

  • Page 3: Table Of Contents

    BX46 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 10, “ASSEMBLY" (pages 31 to 34) carefully. IMPORTANT -- Be sure to read this section for safe use of the equipment. --...
  • Page 4 CAMERA RECORDING TROUBLESHOOTING GUIDE 25-27 SPECIFICATIONS 28,29 OPTICAL CHARACTERISTICS (UIS2 Series) ASSEMBLY 31-34 -- See this section for the replacement of the light bulb. -- HALOGEN LAMP SOCKET INSPECTION SHEET n PROPER SELECTION OF THE POWER SUPPLY CORD ............36,37...

  • Page 5: Important

    4. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual.

  • Page 6: Safety Symbols

    Rear panel position [Caution against high temperature] If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry. Getting Ready (Fig. 2) 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

  • Page 7: Maintenance And Storage

    · The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation. · Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not be assured.

  • Page 8: Module Nomenclature

    } The modules mentioned below show only the typical product names. As there are some products that are not mentioned but also applicable to this microscope, check the latest catalogues or consult Olympus. For the products marked “ * ”, also read their instruction manuals.

  • Page 9: Controls

    BX46 CONTROLS · If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 31 to 34). Interpupillary distance adjustment scale (Page 11) Diopter adjustment ring (Page 19) Allen screwdriver holder Slide holder (Page 17) Coarse adjustment knob...
  • Page 10 Main switch (Page 10) I : ON : OFF Fine adjustment knob (Page 15) Detachable. LIM setting switch (Page 13) Pre-focusing lever (Page 16) LED brightness adjustment knob (Page 13) Top lens swing-out lever (Page 12) LIM ON-OFF switch (Page 13) Condenser height LIM indicator adjustment knob...

  • Page 11: Controls

    BX46 << >> Modules for halogen lamp operation LS30 Adapter Halogen Lamp Socket U-LS30ADP U-LS30-5 Guide pins Guide pin holes Lamp cable Power Supply Unit Lamp socket connector Brightness control knob Main switch I : ON. : OFF.

  • Page 12: Flow Of Observation

    FLOW OF OBSERVATION } When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated. } When the halogen bulb is used, set the 32LBD filter in the filter slider and insert it into the insertion slot @. (Controls Used) (Page) Halogen bulb...
  • Page 13 BX46 (trinocular tube only) } Copy the observation procedure pages on separate sheets and post it near your microscope.

  • Page 14: Simplified Observation Procedure

    SIMPLIFIED OBSERVATION PROCEDURE 4-1 Basic Operation (Until Observation of Specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each control, please read the description page specified below. Press the main switch of the microscope frame to “...

  • Page 15: Microscope Adjustments (How To Improve The Observed Image)

    BX46 Rotate the revolving nosepiece to engage the 10X objective in the light path. Rotate the coarse and fine adjustment knobs to bring the specimen in focus. (Details: Page 19) Rotate the stage knob to adjust the observation position. Now you can observe the magnified image of the specimen. To Fig.

  • Page 16: Adjusting The Centering .................................. 12 4 Adjusting The Contrast

    Adjusting the Centering 10 11 Place the specimen. Rotate the revolving nosepiece to select the 10X objective. Raise the lever to engage the top lens in the light path. Rotate the knobs to bring the specimen in focus. Rotate the knob to raise the condenser to its upper limit. Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field of view.

  • Page 17: Using The Controls

    BX46 USING THE CONTROLS 5-1 Base Adjusting the Brightness (Figs. 10 & 11) 1. When the LED lamp is used for observation, turn the LED brightness adjustment knob @ clockwise to make illumination brighter. 2. When the halogen bulb is used for observation, adjust the brightness adjustment knob 2 of the TL4 power supply unit.

  • Page 18: Using The Filters

    Using the Filters (Fig. 13) } One of the filters listed below can be engaged in the light path by inserting the filter @ in the filter slider 2 and engaging the filter slider in the light path. Usable Filters Applications For light brightness control, transmittance 32LND1.5/3/6/12/25/50...

  • Page 19: Focusing Block

    BX46 5-2 Focusing Block } The stage of this microscope is fixed at a low height to facilitate replacement of specimens. Take care not to let your hand and fingers interfere with the stage when operating the coarse adjustment knob.

  • Page 20: Pre-Focusing Lever

    Replacing the Fine Adjustment Knob (Fig. 17) CAUTION The fine adjustment knob has been attached on the left side at the factory. } The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the X-and Y-axis knobs. Usually attach the fine adjustment knob on the opposite side to the X- and Y-axis knobs.

  • Page 21: Stage

    BX46 5-3 Stage } When the U-SP plain stage is used, place the slide specimen directly on the stage because this model does not have the slide holder. Placing the Specimen (Figs. 20 & 21) } The dimensions of the slide glass should be 26 x 76 mm with thickness of 0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm.

  • Page 22: Adjusting The X- And Y-Axis Knob Tension

    Adjusting the X- and Y-Axis Knob Tension (Fig. 22) 1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose the adjustment knobs. 2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob | clockwise (in the direction of the arrow) increases the tension and counterclockwise decreases it.

  • Page 23: Observation Tube

    BX46 5-4 Observation Tube Adjusting the Diopter (Fig. 24) 1. Set the diopter adjustment rings on both sides to scale “0”. 2. Engage a high-power objective (40X or so) in the light path, look into the right eyepiece with your right eye, and rotate the coarse and fine adjustment knobs to bring the specimen into focus.

  • Page 24: Using The Eyepiece Micrometer

    50% for camera * With the infrared trinocular tube, infrared observation up to 1000 nm is possible. For details, consult your Olympus representative. ** The light path selector knob is removable and can be attached to the other side.

  • Page 25: Adjusting The Tilt

    BX46 Adjusting the Tilt (Figs. 28 to 31) Adjust the height and tilt of the observation tube to obtain the most 5° to 35° comfortable viewing position. U-TBI-3 5° to 35° U-TBI-3-CLI 5° to 35° U-ETBI 0° to 25° U-TTBI 0°...

  • Page 26: Using Eyepieces Incorporating A Micrometer

    Using Eyepieces Incorporating a Micrometer (Fig. 32) } When the eyepieces in use incorporate a micrometer, the accuracy of the left-right focusing adjustment (diopter adjustment) can be improved further. 1. Looking into the right eyepiece with your right eye, turn the top of the eyepiece @ so that the micrometer in the field of view looks sharpest (Fig.

  • Page 27: Immersion Objectives

    BX46 5-6 Immersion Objectives Be sure to use the provided Olympus Immersion oil. CAUTION Using Immersion Objectives (Fig. 34) 1. Focus on the specimen with objectives in the order of lower-power to higher-power ones. 2. Before engaging the immersion objective, place a drop of provided immersion oil onto the specimen at the area to be observed.

  • Page 28: Camera Recording

    CAMERA RECORDING } Use a trinocular observation tube such as the U-TTR-2 when recording video images or digital camera images of microscope images. The trinocular tube accepts a camera adapter (certain camera adapters necessitate a camera mount adapter). } Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not match that of the image observed through eyepieces.

  • Page 29: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance.
  • Page 30 Use immersion oil. with an oil immersion objective. Common Immersion oil contains bubbles. Remove the bubbles. Common Recommended immersion oil is Use an Olympus-designated not used. immersion oil. Clean it. Common Dirt/dust on specimen. Common Dirt/dust on condenser top lens.
  • Page 31 BX46 Problem Lamp Cause Remedy Page Field of view of one eye does not Common Interpupillary distance is incorrect. Adjust interpupillary distance. match that of the other. Common Diopter adjustment is incorrect. Adjust diopter. Common Different eyepieces are used on Change one eyepiece to match left and right.

  • Page 32: Specifications

    SPECIFICATIONS Item Specification 1. Optical system UIS2 (UIS) optical system (featuring infinity correction) 2. Illumination Built-in transmitted Koehler illumination FN (Field Number): 22 (widefield compatible) LED illuminator (continuously variable): U-LHLEDC Optional 6V 30W halogen bulb (continuously variable): 6V30WHAL (PHILIPS 5761) Power supply unit TL4: 100-120/220-240 V $ , 0.85/0.45 A, 50/60 Hz.

  • Page 33: Specifications

    BX46 Item Specification 8. Condenser Type Universal condenser (fixed on frame) N . A. Aperture iris Graduated scale provided diaphragm Filter A 32 mm filter can be engaged in the light path (using the filter slider). Observation method, Transmitted light brightfield observation, applicable 2X - 40X widefield (FN 22).

  • Page 34: Optical Characteristics

    Mechanical tube length Oil: Oil immersion NOTE Iris: Iris diaphragm Refer to the latest catalogue or consult your local Olympus Field number (FN) representative for the updated information on the eyepieces Color band Cover glass thickness and objectives that can be combined with this microscope.

  • Page 35: Assembly Diagram

    The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching CAUTION any parts or touching glass surfaces.
  • Page 36 10-2 Detailed Assembly Procedures Attaching the LED Lamp Housing (Fig. 35) 1. Fit the LED lamp housing @ into the mount hole on the rear of the microscope by aligning the clamping screw 2 and screw hole 3. 2. Using the Allen screwdriver, tighten the clamping screw 2. 3.
  • Page 37 BX46 Attaching the Eyepieces (Fig. 39) Gently insert the eyepieces all the way into the eyepiece sleeves. · When using the U-BI30-2 binocular tube, eyepieces with a po- CAUTION sitioning pin cannot be attached because the U-BI30-2 does not have the positioning notch.
  • Page 38 Attaching the Power Cord (Figs. 41 to 43) CAUTION Always use the power cord provided by Olympus. If no power cord is provided with the microscope, please select the proper power cord by referring to section “ PROPER SELECTION OF THE POWER SUPPLY CORD ”...

  • Page 39: Halogen Lamp Socket Inspection Sheet

    { If there is any ( ) mark noted, immediately stop use of the product, and consult Olympus for detailed inspections or replace the lamp soket. { If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and contact Olympus for detailed inspections.

  • Page 40: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 41 BX46 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoni- Black-Red-Yellow Thread (Length zation Marking (May be located of color section in mm) Approval Organization on jacket or insulation of internal...
  • Page 42 MEMO...
  • Page 44 Printed in Japan on September 14, 2010 M 010-05...

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