Product (Daughter) Ion Mode; Precursor (Parent) Ion Mode - Waters Xevo TQ MS Operator's, Overview And Maintenance Manual

1 Waters Xevo TQ MS Overview

Product (daughter) ion mode

Product ion mode is the most commonly used MS/MS operating mode. You can
specify an ion of interest for fragmentation in the collision cell, thus yielding
structural information.
Product ion mode:
Typical applications
You typically use product ion mode for the following applications:
• Method development for MRM screening studies:
– Identifying product ions for use in MRM transitions.
– Optimizing CID tuning conditions to maximize the yield of a specific
product ion to be used in MRM analysis.
• Structural elucidation (for example, peptide sequencing).

Precursor (parent) ion mode

Precursor ion mode:
34 February 11, 2013, 71500130002 Rev. D
MS1
Static (at precursor mass)
MS1
Scanning
Collision cell
Pass all masses
Collision cell
Pass all masses
MS2
Scanning
MS2
Static (at product mass)