Product (Daughter) Ion Mode - Waters Xevo TQD Overview And Maintenance Manual

Product (daughter) ion mode

Product ion mode is the most commonly used MS/MS operating mode. You can
specify an ion of interest for fragmentation in the collision cell, thus yielding
structural information.
Product ion mode:
Typical applications
Product ion mode is typically used for the following applications:
• Method development for MRM screening studies:
– Identifying product ions for use in MRM transitions.
– Optimizing CID tuning conditions to maximize the yield of a specific
product ion to be used in MRM analysis.
• Structural elucidation (for example, peptide sequencing)
MS1
Static (at precursor mass)
MS/MS operating modes
Collision cell
Fragmenting
precursor ions and
passing all masses
March 14, 2014, 715004387 Rev. B
MS2
Scanning
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