Page 2: Copyright Notice
Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.
Page 3: Customer Comments
Contacting Waters ® Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information...
Page 4: Safety Considerations
Considerations specific to the Xevo TQ-S system Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.
Page 5 Glass breakage hazard To avoid injuries from broken glass, falling objects, or Warning: exposure to toxic substances, never place containers on top of the instrument or on its front covers. High temperature hazard To avoid burn injuries, avoid touching the source ion block Warning: assembly when operating or servicing the instrument.
Page 6: Safety Advisories
The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.
Page 7: Operating This Instrument
This guide is for operators of varying levels of experience. It gives an overview of the instrument and explains how to prepare it for operation, change its modes of operation, and maintain it. Intended use of the Xevo TQ-S ® Waters designed the Xevo...
Page 8: Calibrating
TRIZAIC™ UPLC options, or optional third-party sources (DART , DESI, or LDTD™), the Xevo TQ-S does not comply with the European Union In Vitro Diagnostic Device Directive 98/79/EC. Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve.
Page 9: Ism Classification
Class A products are suitable for use in commercial, (that is, nonresidential) locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Waters Corporation (Micromass UK Ltd.) Floats Road Wythenshawe Manchester M23 9LZ United Kingdom...
Page 11: Table Of Contents
1 Waters Xevo TQ-S Overview ..............1-1 Waters Xevo TQ-S ....................1-2 ACQUITY UPLC/MS Xevo TQ-S systems ............1-4 Non-ACQUITY devices for use with the Xevo TQ-S ........1-6 Software and data system ................1-6 Instrument Console software ................1-7 Ionization techniques and source probes ...........
Page 12 NanoFlow ESI source ..................1-8 Atmospheric solids analysis probe (ASAP)............. 1-9 Atmospheric pressure gas chromatography (APGC) ........1-9 TRIZAIC UPLC source .................. 1-10 IntelliStart Fluidics system ................1-10 Overview......................1-10 System components ..................1-11 System operation ................... 1-11 Ion optics ......................1-12 MS operating modes ..................
Page 13 Leaving the mass spectrometer ready for operation ......2-10 Emergency shutdown of the mass spectrometer ........2-10 3 Changing the Mode of Operation ............3-1 ESI mode ......................3-2 Installing the ESI probe .................. 3-2 Removing the ESI probe.................. 3-6 ESCi mode ......................
Page 14 Preparing the instrument for working on the source ......4-8 Removal and refitting of the source enclosure .......... 4-9 Removing the source enclosure from the instrument ........4-9 Fitting the source enclosure to the instrument..........4-11 Installing and removing the corona pin ............ 4-12 Installing the corona pin in the source ............
Page 15 Cleaning the StepWave ion guide assembly ..........4-59 Handling the StepWave ion guide assembly ..........4-59 Removing the ion block support from the source assembly......4-59 Removing the StepWave assembly from the source assembly ....4-61 Disassembling the StepWave ion guide assembly ........4-64 Cleaning the StepWave ion guide assembly..........
Page 16 Making tubing connections ................B-4 Connecting the Edwards oil-free roughing pumps ........ B-10 Making the roughing pump connections to the Xevo TQ-S ....B-16 Connecting to the nitrogen supply ............B-17 Connecting to the collision cell gas supply ..........B-18 Connecting the nitrogen exhaust line ............
Page 17 Connecting to the electricity source ............B-31 C Materials of Construction and Compatible Solvents ....C-1 Preventing contamination ................C-2 Items exposed to solvent ................C-2 Solvents used to prepare mobile phases ............ C-3 D Plumbing the IntelliStart Fluidics system ........D-1 Preventing contamination ................
Page 18 xviii Table of Contents...
Page 19 Waters Xevo TQ-S Overview This chapter describes the instrument, including its controls and connections for gas and plumbing. Contents Topic Page Waters Xevo TQ-S Ionization techniques and source probes IntelliStart Fluidics system 1-10 Ion optics 1-12 MS operating modes 1-13...
Page 20: Waters Xevo Tq-S Overview
(IonSABRE™ II). • Optional dual-mode APPI/APCI source. • Optional NanoFlow™ ESI source. • Optional TRIZAIC™ UPLC source. • Optional APGC source. You can also use the Xevo TQ-S with the following optional third-party sources: ® • DART • DESI • LDTD™...
Page 21 Waters Xevo TQ-S: IntelliStart technology IntelliStart™ technology monitors LC/MS/MS performance and reports when the mass spectrometer is ready for use. The IntelliStart software automatically tunes and mass calibrates the mass spectrometer and displays performance readbacks. Integrated with MassLynx™ mass spectrometry software and Instrument Console software,...
Page 22: Acquity Uplc/Ms Xevo Tq-S Systems
See the mass spectrometer’s online Help for further details on IntelliStart technology. ACQUITY UPLC/MS Xevo TQ-S systems ® The Waters Xevo TQ-S is compatible with the ACQUITY UPLC systems; if you are not using an ACQUITY UPLC system, refer to the documentation relevant to your LC system (see “Non-ACQUITY devices for use with the Xevo...
Page 23 © Similar to the ACQUITY UPLC system, the nanoACQUITY uses the optional NanoFlow source on the Xevo TQ-S. It is designed for capillary-to-nano-scale separations. Its sensitivity, resolution, and reproducibility well suit it for biomarker discovery and proteomics applications, including protein identification and characterization.
Page 24: Non-Acquity Devices For Use With The Xevo Tq-S
For further instruction, see the nanoACQUITY UPLC System Operator’s Guide or Controlling Contamination in LC/MS Systems (part number 715001307). You can find these documents on http://www.waters.com; click Services and Support > Support. Non-ACQUITY devices for use with the Xevo TQ-S ®...
Page 25: Instrument Console Software
See the MassLynx 4.1 user documentation and online Help for more information on installing and using MassLynx software. OpenLynx™ and TargetLynx™ application managers are included as standard software. Instrument Console software The Instrument Console is a software application with which you configure settings, monitor performance, run diagnostic tests, and maintain the system and its modules.
Page 26: Atmospheric Pressure Chemical Ionization (Apci)
5 to 1000 nL/min. For a given sample concentration, the ion currents observed approximate those seen in normal flow rate electrospray. However, for similar experiments, NanoFlow’s significant reduction in sample consumption accompanies significant increases in sensitivity. Waters Xevo TQ-S Overview...
Page 27: Atmospheric Solids Analysis Probe (Asap)
See the Atmospheric Solids Analysis Probe Operator’s Guide Supplement for further details. Atmospheric pressure gas chromatography (APGC) The Waters APGC couples an Agilent GC with the Xevo TQ-S. Doing so makes possible performing LC and GC analyses in the same system, without compromising performance. The APGC provides complementary information to the LCMS instrument, enabling analysis of compounds of low molecular weight and/or low-to- intermediate polarity.
Page 28: Trizaic Uplc Source
• From a wash reservoir that contains solvent for automated flushing of the instrument’s solvent delivery system. For further information on the IntelliStart Fluidics system, see Appendix “Plumbing the IntelliStart Fluidics system”. 1-10 Waters Xevo TQ-S Overview...
Page 29: System Components
IntelliStart Fluidics system: Probe Diverter valve 6-port selector valve Sample loop Column Waste Wash Pump reservoir reservoir Reservoirs System components The onboard system incorporates a 6-port selector valve, a multi-position diverter valve, a pump, and three sample reservoirs. The sample reservoirs are mounted on the instrument’s front panel. When you select a solvent from the Instrument Console, a light-emitting diode (LED) illuminates the appropriate reservoir.
Page 30: Ion Optics
Any fragment ions can then be mass-analyzed by the second quadrupole. The transmitted ions are detected by the photomultiplier detection system. The signal is amplified, digitized, and sent to the MassLynx mass spectrometry software. 1-12 Waters Xevo TQ-S Overview...
Page 31: Ms Operating Modes
Ion optics: StepWave Sample cone Sample inlet Conjoined T-Wave T-Wave/ScanWave Conversion ion guide ion guide collision cell dynode Isolation valve Z-Spray ion source Source sampling Quadrupole 1 Quadrupole 2 Detector orifice (MS1) (MS2) MS operating modes The following table shows the MS operating modes. MS operating modes: Operating mode Collision cell...
Page 32: Ms/Ms Operating Modes
RADAR™ is an additional mode with which you collect data from the MRM and full scan MS modes simultaneously. RADAR mode can also acquire all detectable ions in both positive and negative full scan MS. 1-14 Waters Xevo TQ-S Overview...
Page 33: Product (Daughter) Ion Mode
Product (daughter) ion mode Product ion mode is the most commonly used MS/MS operating mode. You can specify an ion of interest for fragmentation in the collision cell, thus yielding structural information. Product ion mode: Collision cell Static (at precursor mass) Pass all masses Scanning Typical applications...
Page 34: Precursor (Parent) Ion Mode
Multiple reaction monitoring mode: Collision cell Static (at precursor mass) Pass all masses Static (at product mass) 1-16 Waters Xevo TQ-S Overview...
Page 35: Constant Neutral Loss Mode
Typical application You typically use MRM mode to quantify known analytes in complex samples: • Drug metabolite and pharmacokinetic studies. • Environmental, for example, pesticide and herbicide analysis. • Forensic or toxicology, for example, screening for target drugs in sport. MRM mode does not produce a spectrum, because only one transition is monitored at a time.
Page 36: Scanwave Daughter Scan Mode
1-15. Leak sensors Leak sensors in the drip trays of the Xevo TQ-S continuously monitor the instrument for leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 mL of accumulated leaked liquid in its surrounding reservoir.
Page 37: Rear Panel
Vacuum leaks and electrical or vacuum pump failures cause vacuum loss, which protective interlocks guard against. The system monitors the turbomolecular pump speeds and continuously measures vacuum pressure with built-in Pirani and Penning gauges. The gauges also serve as switches, stopping operation when vacuum loss is sensed.
Page 38 1-20 Waters Xevo TQ-S Overview...
Page 39 Preparing the Mass Spectrometer for Operation This chapter describes how to start and shut down the mass spectrometer. Contents Topic Page Starting the mass spectrometer Preparing the IntelliStart Fluidics system Rebooting the mass spectrometer Leaving the mass spectrometer ready for operation 2-10 Emergency shutdown of the mass spectrometer 2-10...
Page 40: Preparing The Mass Spectrometer For Operation
Starting the mass spectrometer This instrument is compatible with the ACQUITY UPLC system; if you are not using an ACQUITY UPLC system, refer to the documentation relevant to the system you are using (see “Non-ACQUITY devices for use with the Xevo TQ-S”...
Page 41 Press the power switch on the top, left-hand side of the mass spectrometer and ACQUITY instruments. Each system instrument runs a series of startup tests. Result: Allow 3 minutes for the embedded PC to initialize. An audible alert sounds when the PC is ready. The power and operate LEDs change as follows: Tip: •...
Page 42: Verifying The Instrument's State Of Readiness
Verifying the instrument’s state of readiness When the mass spectrometer is in good operating condition, the power and Operate LEDs show constant green. You can view any error messages in IntelliStart software. Monitoring the mass spectrometer LEDs Light-emitting diodes on the mass spectrometer indicate its operational status.
Page 43: Running The Mass Spectrometer At High Flow Rates
Running the mass spectrometer at high flow rates The ACQUITY UPLC system runs at high flow rates. To optimize desolvation, and thus sensitivity, run the ACQUITY Xevo TQ-S system at appropriate gas flows and desolvation temperatures. IntelliStart software automatically sets these parameters when you enter a flow rate, according to the following table.
Page 44: Preparing The Intellistart Fluidics System
Preparing the IntelliStart Fluidics system For additional information, see Appendix D “Connecting the liquid waste line” on page B-22. To avoid accidental spillage damaging the instrument, do not Caution: store large volume solvent reservoirs on top of the instrument. Installing the reservoir bottles Use standard reservoir bottles (30 mL) for instrument setup and calibration.
Page 45: Adjusting The Solvent Delivery Tube Positions
For each reservoir bottle, ensure that the end of the solvent delivery tube is positioned so that it is close to, but does not touch, the bottom of the bottle (see “Adjusting the solvent delivery tube positions” on page 2-7). To install the low-volume vials The reservoir bottles can be contaminated with Warning:...
Page 46: Purging The Infusion Pump
To adjust the position of a solvent delivery tube: Open the access door to the fluidics pump (see the figure on page 1-5). Loosen the finger-tight fitting for the solvent delivery tube you are adjusting. Finger-tight fitting Solvent delivery tube Move the solvent delivery tube so that its end is close to, but does not touch, the bottom of the reservoir bottle or low volume vial.
Page 47: Rebooting The Mass Spectrometer
Rebooting the mass spectrometer The reset button shuts down the electronics momentarily and causes the mass spectrometer to reboot. Reboot the mass spectrometer when either of these conditions applies: • The MassLynx software fails to initialize. • Immediately following a software upgrade. To reboot the mass spectrometer: Ensure that the MassLynx software is closed.
Page 48: Leaving The Mass Spectrometer Ready For Operation
Leaving the mass spectrometer ready for operation Leave the mass spectrometer in Operate mode except in the following cases: • When performing routine maintenance • When changing the source • When leaving the mass spectrometer unused for a long period In these instances, put the mass spectrometer in Standby mode (see the online Help for details).
Page 49 • APCI (atmospheric pressure chemical ionization) • Combined Atmospheric Pressure Photoionization (APPI/APCI) • NanoFlow ESI For details about other Waters and third party source options, refer to the documentation supplied with the source. Contents Topic Page ESI mode ESCi mode...
Page 50: Changing The Mode Of Operation
ESI mode The standard ESI probe (fitted to the instrument when it is shipped from the factory) accommodates eluent flow rates as fast as 2 mL/min. For further details, see “Electrospray ionization (ESI)” on page 1-7. The following sections explain how to install and remove an ESI probe. Installing the ESI probe Required materials •...
Page 51 With the probe label facing you, carefully slide the ESI probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with the location hole of the probe adjuster assembly. Probe label Probe location dowel Location hole of the probe adjuster assembly TP03129...
Page 52 ESI probe, mounted on the source enclosure: Vernier probe adjuster ESI probe Probe locking ring ESI probe cable Vertical probe High voltage adjuster connector Source window Source enclosure release TP03128 To avoid nitrogen leakage, fully tighten the probe locking Caution: ring.
Page 53 To reduce peak broadening, use 0.004-inch ID tubing Recommendation: for sample flow rates 1.2 mL/min; use 0.005-inch ID tubing for sample flow rates 1.2 mL/min. Requirements: • If you are replacing the tubing supplied with the instrument, you must minimize the length of the tube connecting the diverter valve to the ESI probe.
Page 54: Removing The Esi Probe
Removing the ESI probe Required materials Chemical-resistant, powder-free gloves To remove the ESI probe: The LC system connections, ESI probe, and source can Warning: be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is Warning: prepared for working on the source before commencing this procedure.
Page 55: Apci Mode
APCI mode APCI mode, an option for the mass spectrometer, produces singly-charged protonated or deprotonated molecules for a broad range of nonvolatile analytes. The APCI interface consists of the ESI/APCI/ESCi enclosure fitted with a corona pin and an IonSABRE II probe. Mobile phase from the LC column enters the probe, where it is pneumatically converted to an aerosol, rapidly heated, and vaporized or gasified at the probe tip.
Page 56 To install the IonSABRE II probe: The LC system connections, IonSABRE II probe, and Warning: source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is Warning: prepared for working on the source before commencing this procedure.
Page 57 IonSABRE II probe mounted on the source enclosure: IonSABRE II probe Probe locking ring Vernier probe adjuster Vertical probe adjuster Source window Source enclosure release TP03128 Open the access door to the fluidics valve (see the figure on page 1-5). To avoid electric shock, do not use stainless steel tubing Warning: to connect the diverter valve to the IonSABRE II probe;...
Page 58: Removing The Ionsabre Ii Probe
Removing the IonSABRE II probe Required materials Chemical-resistant, powder-free gloves To remove the IonSABRE II probe: The LC system connections, IonSABRE II probe, and Warning: source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is Warning: prepared for working on the source before commencing this procedure.
Page 59: Combined Appi/Apci Source
Combined APPI/APCI source Operate this optional, replacement source enclosure as APPI, APCI, or dual-mode APPI/APCI. Where, dual-mode APPI/APCI performs rapid switching between ionization modes. APPI operation In atmospheric pressure photoionization (APPI) mode, the source is fitted with an IonSABRE II probe, and the APPI lamp drive assembly is advanced into the source.
Page 60: Apci Operation
APCI operation Atmospheric pressure chemical ionization (APCI) produces singly-charged protonated or deprotonated molecules for a large range of nonvolatile analytes. In APCI mode, the source is fitted with an APCI corona pin. Unused, the APPI lamp drive assembly is retracted from the source. APCI mode: IonSABRE II probe Sample cone...
Page 61: Dual-Mode Operation
Dual-mode operation Dual-mode operation enables rapid switching between APPI and APCI ionization modes and allows high-throughput operations (for example, for sample screening). You replace the standard corona pin with a specially shaped APPI/APCI corona pin, so that the APPI lamp holder can be advanced into the source for dual operation.
Page 62: The Combined Appi/Apci Source Components
The combined APPI/APCI source components The combined APPI/APCI source comprises the standard IonSABRE II probe and a source enclosure with an APPI lamp drive incorporated. The combined APPI/APCI source enclosure: APPI lamp drive assembly To prevent damage to the corona pin and lamp assembly, Caution: ensure that the lamp assembly does not touch the corona pin when the source enclosure door is closed.
Page 63 APPI lamp drive assembly inside the source enclosure: APPI lamp drive assembly Source enclosure IonSABRE II probe UV lamp and repeller electrode TP03201 Combined APPI/APCI source 3-15...
Page 64: Installing The Combined Appi/Apci Source
Installing the combined APPI/APCI source Required materials Chemical-resistant, powder-free gloves To install the combined APPI/APCI source: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is suitably Warning: prepared before commencing this procedure.
Page 65: Removing The Ionsabre Ii Probe And Combined Appi/Apci Source Enclosure
Slide closed the instrument’s source interface door. 10. Install the IonSABRE II probe to the source, and ensure that it is working correctly (see page 3-8). An automatic pressure test is performed each time the source Tip: enclosure is closed, and when the instrument starts. Removing the IonSABRE II probe and combined APPI/APCI source enclosure Required materials...
Page 66: Nanoflow Esi Source
NanoFlow ESI source The NanoFlow source enclosure comprises the NanoFlow stage (for x-, y-, z-axis adjustment), the sprayer-enclosure, and a microscope camera. NanoFlow source, stage and microscope camera: Microscope camera Sprayer enclosure X, Y, Z stage TP03199 A sprayer is mounted on an X, Y, Z stage (three-axis manipulator) which slides on a pair of guide rails that allow its withdrawal from the source enclosure for maintenance and changes.
Page 67: Installing The Nanoflow Source
Installing the NanoFlow source Required materials Chemical-resistant, powder-free gloves To install the NanoFlow source: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is suitably Warning: prepared before commencing this procedure.
Page 68 For details regarding how to fit each sprayer, see the corresponding Tip: reference: • Waters Universal NanoFlow Sprayer Installation and Maintenance Guide (part number 71500110107) • “Fitting a borosilicate glass capillary (nanovial)” on page 3-21 • Capillary Electrophoresis/Capillary Electrochromatography...
Page 69: Fitting A Borosilicate Glass Capillary (Nanovial)
Slide open the instrument’s source interface door (see the figure on page 1-5). Connect the probe cable to the instrument’s PROBE connection. The NanoFlow stage contains a high voltage interlock so Caution: that unless the sprayer is pushed fully forward in the source, the capillary voltage (the voltage applied to the sprayer assembly) and the sampling cone voltage are disabled.
Page 70 ® • Fused silica syringe needle or GELoader • Fused silica cutter To fit a borosilicate glass capillary (nanovial): To avoid lacerations, puncture injuries, and possible Warning: contamination with biohazardous and toxic samples, do not touch the sharp end of the capillary. Capillaries are extremely fragile.
Page 71 Remove the existing capillary from the sprayer. Carefully remove the new borosilicate glass capillary from its case by lifting vertically while pressing on the foam with two fingers. Foam Capillary Load sample into the capillary using a fused silica syringe needle or a GELoader tip, minimizing any bubbles between the capillary tip and the sample.
Page 72 Sprayer Assembly: PTFE tubing Ferrule Union Knurled nut Blue conductive elastomer Glass capillary 5 mm 12. Screw the sprayer back into the assembly. 13. Replace the sprayer cover. 14. On the MassLynx MS Tune window, ensure that the Capillary parameter on the ES+/- Source tab is set to 0 kV. To ensure that the capillary tip does not collide with the Caution: cone or the side of the source, adjust the sprayer tip position before...
Page 73: Positioning The Borosilicate Glass Capillary Tip
2 mm. Capillary tip position: Cone aperture diameter For tuning instructions, see the MassLynx, Xevo TQ-S online help, “Tuning manually for NanoFlow operation”. Restarting a stalled borosilicate glass capillary electrospray Should the spray stop, you can restart it. To do so, in the Tune window, set Capillary to 0 kV.
Page 74 3-26 Changing the Mode of Operation...
Page 75: Maintenance Procedures
Maintenance Procedures This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents Topic Page Maintenance schedule Spare parts Troubleshooting with Connections INSIGHT Safety and handling Preparing the instrument for working on the source Removal and refitting of the source enclosure...
Page 76 Contents (Continued) Topic Page Replacing the IonSABRE II probe sample capillary 4-91 Cleaning or replacing the corona pin 4-97 Replacing the IonSABRE II probe heater 4-98 Replacing the ion block source heater 4-101 Replacing the source assembly seals 4-105 Replacing the air filter 4-108 APPI/APCI source—changing the UV lamp bulb 4-110...
Page 77: Maintenance Schedule
Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance. Maintenance schedule: Procedure Frequency For information... Clean the instrument case. As required. page 4-20. Empty the nitrogen exhaust Check daily, empty as page 4-20. trap bottle. required.
Page 78 Maintenance schedule: (Continued) Procedure Frequency For information... Clean the IonSABRE II probe When sensitivity page 4-90. tip. (Options using the decreases to IonSABRE II probe only.) unacceptable levels or when significant chemical interference is present. Replace the IonSABRE II When sensitivity page 4-91.
Page 79: Spare Parts
4-114. assembly O-rings. Spare parts Waters recommends that you replace only the parts mentioned in this document. For spare parts details, see the Waters Quality Parts Locator on the Waters Web site’s Services/Support page. Troubleshooting with Connections INSIGHT ® Connections INSIGHT is an “intelligent”...
Page 80 A .zip file containing your Connections Insight profile is Result: forwarded to Waters customer support for review. Saving a service profile or plot file from the Instrument Console can require up to 150 MB of file space. Maintenance Procedures...
Page 81: Safety And Handling
Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: The instrument components can be contaminated with Warning: biologically hazardous materials. Always wear chemical-resistant, powder-free gloves while handling the components. To prevent injury, always observe Good Laboratory Practices Warning: when handling solvents, changing tubing, or operating the instrument.
Page 82: Preparing The Instrument For Working On The Source
In the Instrument Console, click Stop Flow to stop the LC flow, or if column flow is required, divert the LC flow to waste as follows: In the Instrument Console system tree, expand Xevo TQ-S Detector, Interactive Fluidics. Click Control Select Waste as the flow state.
Page 83: Removal And Refitting Of The Source Enclosure
Removal and refitting of the source enclosure The optional combined APPI/APCI and NanoFlow sources are supplied as a complete source enclosure. To fit them, you must first remove the standard source enclosure. Removing the source enclosure from the instrument Required materials Chemical-resistant, powder-free gloves To remove the source enclosure: The source components can be contaminated with...
Page 84 Using both hands, grasp the source enclosure, and lift it vertically off the two supporting studs on the source adaptor housing. Cable storage positions Supporting stud TP03164 Source enclosure Store the cables neatly by plugging them into the cable-storage positions on the rear of the source enclosure.
Page 85: Fitting The Source Enclosure To The Instrument
Fitting the source enclosure to the instrument Required materials Chemical-resistant, powder-free gloves To fit the source enclosure: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid puncture wounds, take great care while fitting the Warning: source enclosure to the source if a corona pin is fitted (the pin tip is sharp).
Page 86: Installing And Removing The Corona Pin
Installing and removing the corona pin For ESCi, APCI and dual-mode APCI/APPI operation, you must fit a corona pin. Installing the corona pin in the source Required materials Chemical-resistant, powder-free gloves To install the corona pin in the source: The LC system connections, ESI probe, and source can Warning: be contaminated with biohazardous and/or toxic materials.
Page 87 Corona pin mounting contact: Corona pin mounting contact blanking plug TP03130 The corona pin tip is sharp. To avoid puncture wounds, Warning: handle it with care. Fit the corona pin to the mounting contact, ensuring that the corona pin is securely mounted. Corona pin: Corona pin TP03130...
Page 88 Close the source enclosure. Look through the source window, and use the vernier probe adjuster to position the probe tip so that it is pointing approximately midway between the tips of the sample cone and corona pin. Vernier probe adjuster Source window TP03128 4-14...
Page 89: Removing The Corona Pin From The Source
Removing the corona pin from the source Required materials Chemical-resistant, powder-free gloves To remove the corona pin from the source: The LC system connections, ESI probe, and source can Warning: be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
Page 90: Operating The Source Isolation Valve
Operating the source isolation valve You must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. Required materials Chemical-resistant, powder-free gloves To close the source isolation valve before starting a maintenance procedure: The source components can be contaminated with Warning:...
Page 91 Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Isolation valve handle in closed position Operating the source isolation valve 4-17...
Page 92 To open the source isolation valve after completing a maintenance procedure: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid puncture wounds, take great care while working with Warning: the source enclosure open if one or both of these conditions apply: •...
Page 93: Removing O-Rings And Seals
Removing O-rings and seals When performing certain maintenance procedures, you must remove O-rings or seals from instrument components. An O-ring removal kit is provided with the instrument. O-ring removal kit: Tool 1 Tool 2 To remove an O-ring: When removing an O-ring or seal from a component, be careful Caution: not to scratch the component with the removal tool.
Page 94: Cleaning The Instrument Case
Cleaning the instrument case Do not use abrasives or solvents to clean the instrument’s case. Caution: Use a soft cloth, dampened with water, to clean the outside surfaces of the mass spectrometer. Emptying the nitrogen exhaust trap bottle Inspect the nitrogen exhaust trap bottle in the instrument’s exhaust line daily, and empty it before it is more than 10% full.
Page 95 Required materials Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: In the Instrument Console, click Stop Flow to stop the LC flow. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. The waste liquid in the nitrogen exhaust trap Warning: bottle comprises ACQUITY UPLC solvents and analytes.
Page 96: Maintaining The Oerlikon Leybold Oil-Filled Roughing Pumps
Maintaining the Oerlikon Leybold oil-filled roughing pumps In addition to the roughing pump maintenance requirements Requirement: detailed here, refer to the manufacturer’s documentation provided with the instrument. Oerlikon Leybold roughing pump: Oil filler plug Oil level sight glass Gas ballast valve Oil drain plug TP03296 4-22...
Page 97: Gas Ballasting The Oerlikon Leybold Roughing Pumps
Gas ballasting the Oerlikon Leybold roughing pumps Failure to routinely gas ballast the roughing pumps shortens Caution: oil life and, consequently, pump life. The roughing pumps draw large quantities of solvent vapors that condense in the pump oil, diminishing pumping efficiency. Gas ballasting purges condensed contaminants from the oil.
Page 98: Inspecting The Roughing Pump Oil Levels
Inspecting the roughing pump oil levels To ensure correct operation of the roughing pumps, do not Caution: operate them with the oil level at less than 30% of the maximum level, as indicated in the pumps’ sight glasses. This procedure does not apply to an Edwards oil-free roughing pump. Note: You must determine the oil levels while the roughing pumps Requirement:...
Page 99 To add oil to a roughing pump: Vent and shut down the mass spectrometer (see the mass spectrometer’s online Help for details). To avoid personal injury, as well as damage to the Warning: roughing pumps and mass spectrometer, disconnect the power cords for the mass spectrometer and roughing pumps from the main power source.
Page 100 To avoid oil leakage when fitting the oil filler plug to the Caution: roughing pump, • inspect the O-ring on the plug, and verify that it is free of particles. • ensure that the plug is not cross threaded. • do not overtighten the plug. Use the 12-mm Allen wrench to refit the oil filler plug.
Page 101: Replacing The Roughing Pumps' Oil And Oil Demister Elements
Replacing the roughing pumps’ oil and oil demister elements Replace the roughing pumps’ oil and oil demister elements annually. This procedure is not required for an Edwards oil-free roughing pump. Note: Required materials • Chemical-resistant, powder-free gloves • 12-mm Allen wrench •...
Page 102 To drain the roughing pumps’ oil: The roughing pump oil can be contaminated with Warning: analyte accumulated during normal operation. Always wear chemical-resistant, powder-free gloves when adding or replacing oil. To avoid burn injuries, take great care while working with the Warning: roughing pumps: they can be hot.
Page 103 To avoid oil leakage when fitting the oil drain plug to the Caution: roughing pump, • ensure that the plug is not cross threaded. • ensure that the O-ring is not pinched. • do not overtighten the plug. Use the 12-mm Allen wrench to refit the oil drain plug. When the plug is tightened, it seals against an O-ring.
Page 104 To avoid oil leakage when fitting the oil filler plug to the Caution: roughing pump, • ensure that the plug is not cross threaded. • ensure that the O-ring is not pinched. • do not over tighten the plug. Use the 12-mm Allen wrench to refit the oil filler plug. When the plug is tightened, it seals against an O-ring.
Page 105 Using both hands, carefully remove the exhaust flange and oil demister element from the roughing pump. Oil demister element TP02693 Use the 10-mm wrench to remove the nut that secures the oil demister element to the exhaust flange. Spring Securing nut TP02686 Maintaining the Oerlikon Leybold oil-filled roughing pumps 4-31...
Page 106 Holding the oil demister element slightly elevated, to prevent the loss of the spring, remove its flange. TP02692 Remove the spring from the oil demister element. The oil demister element can be contaminated with Warning: biohazardous and/or toxic materials. Ensure that it is correctly disposed of according to local environmental regulations.
Page 107 To fit the new oil demister elements: The pump oil can be contaminated with analyte Warning: accumulated during normal operation. Always wear chemical-resistant, powder-free gloves when replacing the oil demister element. Do as follows for each pump: Fit the spring to the new oil demister element. TP02682 Holding the oil demister element slightly elevated, to prevent the loss of the spring, fit its exhaust flange.
Page 108 Do not overtighten the nut that secures the oil demister Caution: element to the exhaust flange. Ensure that only (approximately) 1 mm of exposed thread appears beyond the nut when it is tightened. Use the 10-mm wrench to fit and tighten the nut that secures the oil demister element to the exhaust flange.
Page 109 To prepare for operation after changing the roughing pumps’ oil and oil demister elements: Connect the power cords for the mass spectrometer and both roughing pumps to the main power source. Start the mass spectrometer (see page 2-2). Gas-ballast the roughing pumps (see page 4-24).
Page 110: Gas Ballasting The Edwards Xds46I Oil-Free Roughing Pump
Gas ballasting the Edwards XDS46i oil-free roughing pump In addition to the roughing pump gas ballasting procedure Requirement: detailed here, please refer to the manufacturer’s documentation provided with the instrument. Failure to routinely gas ballast the roughing pump shortens Caution: pump life.
Page 111: Cleaning The Source Components
Use of the gas ballast control To select no-gas ballast, turn the control position to 0. Doing so pumps gases fully, achieving ultimate vacuum. To select low-flow gas ballast, turn the control to position I. Use this position for these purposes: •...
Page 112: Cleaning The Sampling Cone Assembly
Cleaning the sampling cone assembly You can remove the sampling cone assembly (comprising the sample cone, O-ring, and cone gas nozzle) for cleaning without venting the instrument. Removing the sampling cone assembly from the source Required materials Chemical-resistant, powder-free gloves To remove the sampling cone assembly from the source: The source components can be contaminated with Warning:...
Page 113 Close the source isolation valve (see page 4-16). Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly 90 degrees, moving the handle from the vertical to the horizontal position. Sampling cone assembly Cone gas nozzle handle TP03131 Do not open the isolation valve at any time when the...
Page 114: Disassembling The Sampling Cone Assembly
Disassembling the sampling cone assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To disassemble the sampling cone assembly: The sampling cone assembly can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
Page 115 The sample cone is fragile. Never place it on its tip; Caution: always place it on its flanged base. Rotate and lift the tool and collar to remove the sample cone from the cone gas nozzle. Remove the O-ring from the sample cone. Cone gas nozzle Cone gas nozzle handle O-ring...
Page 116: Cleaning The Sample Cone And Cone Gas Nozzle
The O-ring can be contaminated with Warning: biohazardous and/or toxic materials. Dispose of it according to local environmental regulations. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Unscrew and remove the PEEK cone gas nozzle handle from the cone gas nozzle.
Page 117 To clean the sample cone and cone gas nozzle: The sample cone and cone gas nozzle can be Warning: contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Formic acid is extremely corrosive and toxic. Work with Warning: extreme care, use a fume hood and suitable protective equipment.
Page 118: Assembling The Sampling Cone Assembly
Assembling the sampling cone assembly Required materials Chemical-resistant, powder-free gloves To assemble the sampling cone assembly: Caution: • To avoid recontaminating the sampling cone assembly, wear clean chemical-resistant, powder-free gloves during this procedure. • The sample cone is fragile. Never place it on its tip; always place it on its flanged base.
Page 119: Fitting The Sampling Cone Assembly To The Source
Fitting the sampling cone assembly to the source Required materials Chemical-resistant, powder-free gloves To fit the sampling cone assembly to the source: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
Page 120 Ensure that the source isolation valve is in the closed position (see page 4-16). Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly. Ion block assembly TP03132 Sampling cone assembly...
Page 121: Cleaning The Ion Block Assembly
Cleaning the ion block assembly Clean the ion block assembly if cleaning the sample cone and cone gas nozzle fails to increase signal sensitivity. Removing the ion block assembly from the source assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To remove the ion block assembly: The source components can be contaminated with...
Page 122 Removing the source enclosure aids access to the ion block Rationale: assembly. Close the source isolation valve (see page 4-16). Use the combined 2.5-mm Allen wrench and cone extraction tool to unscrew the 4, captive, ion block assembly securing screws. Ion block assembly securing screws TP03130...
Page 123: Disassembling The Source Ion Block Assembly
Remove the ion block assembly from the PEEK ion block support. PEEK ion block support Ion block assembly TP03130 Disassembling the source ion block assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool •...
Page 124 To disassemble the ion block assembly: The ion block assembly can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves to perform this procedure. Ensure that the source isolation valve is closed. Source isolation valve handle in closed position Sampling cone assembly retaining...
Page 125 Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 ion block cover plate captive securing screws. Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Grasp the isolation valve, and pull it out of the ion block. Isolation valve O-ring Cleaning the ion block assembly...
Page 126 Use the O-ring removal kit to carefully remove the isolation valve O-ring (see page 4-19). The isolation valve O-ring can be contaminated Warning: with biohazardous and/or toxic materials. Dispose of it according to local environmental regulations. If the isolation valve O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.
Page 127 To avoid damaging the heater cartridge assembly wires, Caution: do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block. 10. Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block.
Page 128 11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also “Removing O-rings and seals” on page 4-19). Cover seal Cone gas O-ring 12. Use the O-ring removal kit to carefully remove the cone gas O-ring from the ion block.
Page 129: Cleaning The Ion Block Components
Cleaning the ion block components Required materials • Chemical-resistant, powder-free gloves. • Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. • HPLC-grade (or better) methanol. • HPLC-grade (or better) water. •...
Page 130: Assembling The Source Ion Block Assembly
If you used formic acid in the cleaning solution, do as follows: Rinse the components by immersing them separately in glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. Dry the components by immersing them in separate glass vessels containing methanol and then placing the vessels in the ultrasonic bath for 10 minutes.
Page 131 To assemble the ion block assembly: Caution: • To avoid recontaminating the ion block assembly, wear clean, chemical-resistant, powder-free gloves during this procedure. • To avoid damaging the heater cartridge assembly wires, do not bend or twist them when fitting the assembly and ceramic heater mounting block to the ion block.
Page 132: Fitting The Ion Block Assembly To The Source Assembly
Fitting the ion block assembly to the source assembly Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool To fit the ion block assembly to the source assembly The source components can be contaminated with Warning: biohazardous and/or toxic materials.
Page 133: Cleaning The Stepwave Ion Guide Assembly
Cleaning the StepWave ion guide assembly Clean the StepWave ion guide assembly if cleaning the ion block and isolation valve fails to increase signal sensitivity. Handling the StepWave ion guide assembly The StepWave ion guide assembly is fragile; handle it and its Caution: components carefully throughout the cleaning procedure.
Page 134 Use the 3-mm Allen wrench to unscrew and remove the 4 screws securing the PEEK ion block support to the adaptor housing. Adaptor housing PEEK ion block support Securing screws StepWave assembly Remove the PEEK ion block support from the adaptor housing. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see page...
Page 135: Removing The Stepwave Assembly From The Source Assembly
Removing the StepWave assembly from the source assembly Required materials • Chemical-resistant, powder-free gloves • Seal breaker and locator tool • StepWave assembly removal and insertion tool When not in use, the seal breaker and locator tool may be stored on the Tip: end of the StepWave assembly removal and insertion tool.
Page 136 Seal break and locator tool: Handle Seal breaker and locator tool positioned on the adaptor housing: Ion guide cap Adaptor housing Seal breaker and locator tool 4-62 Maintenance Procedures...
Page 137 Push firmly on the seal breaker and locator tool’s handle, to lever the StepWave assembly slightly out of the adaptor housing. Moving the assembly in this manner releases it from a seal Rationale: located inside the instrument. With the StepWave removal and insertion tool’s cutout uppermost, insert the tool’s pins into the ion block support screw holes above and below the aperture in the pumping block assembly.
Page 138: Disassembling The Stepwave Ion Guide Assembly
The StepWave ion guide assembly is fragile; handle it Caution: with care. Remove the StepWave ion guide assembly from the StepWave removal and insertion tool. Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. Close the source enclosure.
Page 139 To disassemble the StepWave ion guide assembly: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. The StepWave ion guide assembly is fragile; handle it and its Caution: components carefully throughout this procedure.
Page 140 Separate the first and second ion guide assemblies. First ion guide assembly Second ion guide assembly Lay the clean, lint-free cloth on a bench, and then place the second ion guide assembly, upside down, on the cloth. To avoid damaging the second ion guide assembly, Caution: prevent the support rods turning when removing the screws that secure the differential aperture to the assembly.
Page 141 On the second ion guide assembly, insert the 3-mm Allen wrench through one of the holes in one of the support rods. 3-mm Allen wrench Support rod Brown PEEK gasket 2.5-mm Allen wrench and cone extraction tool While using the 3-mm Allen wrench as a lever to stop the support rod turning, use the combined 2.5-mm Allen wrench and cone extraction tool to remove one of the screws securing the brown PEEK gasket to the second ion guide assembly.
Page 142 Remove the brown PEEK gasket and insulators from the second ion guide assembly. Brown PEEK gasket PEEK insulators Second ion guide assembly Remove the O-ring from the differential pumping aperture. Differential pumping aperture O-ring Slotted screw The O-ring can be contaminated with Warning: biohazardous and/or toxic materials.
Page 143: Cleaning The Stepwave Ion Guide Assembly
10. Use the small, flat-blade screwdriver to remove the 4 slotted screws that secure the differential pumping aperture to the second ion guide PCB assembly. 11. Remove the differential pumping aperture, complete with support rods, from the second ion guide PCB assembly. Intermediate differential aperture Second ion guide PCB assembly...
Page 144 • Appropriately sized glass vessels in which to completely immerse the smaller StepWave assembly components when cleaning. Use only glassware not previously cleaned with surfactants. • Two lengths of small diameter PEEK or PTFE tubing appropriately sized for suspending the StepWave first ion guide and second ion guide PCB assemblies in the glass vessels when cleaning.
Page 145 Hook First ion guide assembly Add 1:1 methanol/water to the glass vessel until the first ion guide assembly is immersed completely. Repeat step 1 through step 3 for the second ion guide PCB assembly, placing the hook through one of the screw holes on the assembly. Hook Second ion guide PCB assembly Cleaning the StepWave ion guide assembly...
Page 146 Place the vessels containing the first ion guide and second ion guide PCB assemblies in the ultrasonic bath for 30 minutes. To avoid recontaminating the first ion guide and second Caution: ion guide PCB assemblies, wear clean, chemical-resistant, powder-free gloves for the rest of this procedure. Carefully remove the first ion guide assembly from its vessel, and blow-dry it using inert, oil-free gas.
Page 147: Assembling The Stepwave Ion Guide Assembly
If you used formic acid in the cleaning solution, rinse the differential pumping aperture by immersing it, with support rods, in a glass vessel containing water and then placing the vessel in the ultrasonic bath for 20 minutes. To avoid recontaminating the first ion guide assembly, Caution: wear clean, chemical-resistant, powder-free gloves for the following step.
Page 148 Fit the brown PEEK gasket and insulators to the second ion guide assembly. Ensure that the brown PEEK gasket is orientated correctly. Tip: Brown PEEK gasket PEEK insulators On the second ion guide assembly, insert the 3-mm Allen wrench through one of the holes in one of the support rods. To avoid damaging the second ion guide assembly, Caution: prevent the support rods turning when fitting the screws that...
Page 149: Fitting The Stepwave Assembly To The Source Assembly
Fitting the StepWave assembly to the source assembly Required materials • Chemical-resistant, powder-free gloves • Seal breaker and locator tool • StepWave assembly removal and insertion tool To fit the StepWave assembly to the source assembly: The source components can be contaminated with Warning: biohazardous and/or toxic materials.
Page 150 StepWave assembly Cutout Pins StepWave assembly removal and insertion tool With the StepWave removal and insertion tool’s cutout uppermost, insert the StepWave removal and insertion tool’s pins into the ion block support screw holes above and below the aperture in the pumping block assembly.
Page 151: Fitting The Ion Block Support To The Source
Invert the seal breaker and locator tool, and locate it over the end of the StepWave assembly. Inverted seal breaker and locator tool StepWave assembly Adaptor housing Push firmly on the seal breaker and locator tool until the tool’s face contacts the adaptor housing.
Page 152: Replacing The Esi Probe Tip And Gasket
To fit the PEEK ion block support to the source: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Ensure that the grooves for the PEEK ion block support O-rings are free from dirt and debris.
Page 153 To remove the ESI probe tip and gasket: The probe and source components can be contaminated Warning: with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. The probe and source can be hot. To avoid burn injuries, take Warning: great care while performing this procedure.
Page 154 If the probe tip is difficult to remove, use the 7-mm wrench in Tip: conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip Remove the metal gasket from the probe tip. Metal gasket The probe tip and metal gasket can be Warning: contaminated with biohazardous and/or toxic materials.
Page 155: Fitting The Esi Probe Tip And Gasket
Fitting the ESI probe tip and gasket Required materials • Chemical-resistant, powder-free gloves • 10-mm wrench • New metal gasket To fit the ESI probe tip and gasket The probe and source components can be contaminated Warning: with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
Page 156: Replacing The Esi Probe Sample Capillary
Replacing the ESI probe sample capillary Replace the stainless steel sample capillary in the ESI probe if it becomes blocked and cannot be cleared, or if it becomes contaminated or damaged. Removing the existing capillary Required materials • Chemical-resistant, powder-free gloves •...
Page 157 Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 probe end-cover retaining screws. End-cover retaining screws Remove the end cover and gasket from the probe assembly. Nebulizer adjuster knob Gasket End cover Unscrew and remove the nebulizer adjuster knob. Replacing the ESI probe sample capillary 4-83...
Page 158 Use the 10-mm wrench to remove the probe tip. 10-mm wrench Probe tip If the probe tip is difficult to remove, use the 7-mm wrench in Tip: conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip 4-84 Maintenance Procedures...
Page 159 Remove the metal gasket from the probe tip. Metal gasket Remove the PEEK union/UNF coupling assembly and capillary from the probe. PEEK union/UNF coupling assembly Capillary Unscrew and remove the knurled collar from the UNF coupling. PEEK union UNF coupling Locknut Knurled collar Conductive sleeve...
Page 160: Installing The New Capillary
12. Unscrew the finger-tight PEEK union from the UNF coupling. Ferrule PTFE liner sleeve 13. Remove the ferrule and PTFE liner sleeve from the capillary. 14. Remove the capillary from the UNF coupling. The capillary, PTFE liner sleeve, and ferrule can Warning: be contaminated with biohazardous and/or toxic materials.
Page 161 • Sharp knife or PEEK tubing cutter • Metal gasket for the probe tip • Safety goggles To install the new capillary The probe and source components can be contaminated Warning: with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
Page 162 Fit the UNF coupling to the new capillary. Use the needle-nose pliers to slide a new liner sleeve and ferrule onto the capillary. Insert the capillary in the PEEK union, and ensure that it is fully seated. Screw the UNF coupling into the PEEK union, finger-tight only. Gently tug on the capillary, testing to ensure that it stays in place.
Page 163 15. Carefully push the PEEK union/UNF coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. UNF coupling locating pin Probe assembly locating slot 16.
Page 164: Cleaning The Ionsabre Ii Probe Tip
See the mass spectrometer’s online Help for further details. To clean the IonSABRE II probe tip On the Instrument Console system tree, click Xevo TQ-S > Manual optimization. On the manual optimization page, click to stop the liquid flow.
Page 165: Replacing The Ionsabre Ii Probe Sample Capillary
Replacing the IonSABRE II probe sample capillary Replace the stainless steel sample capillary in the IonSABRE II probe if it becomes blocked and you cannot clear it, or if it becomes contaminated or damaged. Removing the existing capillary Required materials •...
Page 166 Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 probe end-cover retaining screws. End-cover retaining screws Remove the end cover and gasket. Nebulizer adjuster knob Gasket End-cover Unscrew and remove the nebulizer adjuster knob. Remove the PEEK union/UNF coupling assembly and capillary from the probe.
Page 167: Installing The New Capillary
The PEEK union used with the IonSABRE II probe is notched on Tip: one of its flats, a feature that distinguishes it from the PEEK union used with the ESI probe (see “Replacing the ESI probe sample capillary” on page 4-82).
Page 168 • Needle-nose pliers • 7-mm wrench • Combined 2.5-mm Allen wrench and cone extraction tool • Red PEEK tubing • LC pump • HPLC-grade (or better) 1:1 acetonitrile/water • Capillary • Sharp knife or PEEK tubing cutter • Safety goggles To install the new capillary: The probe and source components can be contaminated Warning:...
Page 169 Fit the UNF coupling to the new capillary. Requirement: Use a UNF coupling with no grooves, which is appropriate to the IonSABRE II probe. Use the needle-nose pliers to slide a new ferrule onto the capillary. Insert the capillary in the PEEK union, and ensure that it is fully seated.
Page 170 15. Carefully push the PEEK union/UNF coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. Probe assembly locating slot UNF coupling locating pin 16.
Page 171: Cleaning Or Replacing The Corona Pin
Cleaning or replacing the corona pin Required materials • Chemical-resistant, powder-free gloves • Needle-nose pliers • HPLC-grade (or better) methanol • Lint-free tissue • Lapping film • Corona pin To clean or replace the corona pin: The probe and source components can be contaminated Warning: with biohazardous and/or toxic materials.
Page 172: Replacing The Ionsabre Ii Probe Heater
Replacing the IonSABRE II probe heater Replace the IonSABRE II probe heater if it fails to heat the probe. Removing the IonSABRE II probe heater Required materials Chemical-resistant, powder-free gloves To remove the IonSABRE II probe heater: The probe and source components can be contaminated Warning: with biohazardous and/or toxic materials.
Page 173 Take great care not to damage the probe heater’s Caution: electrical connections, do not twist the heater when removing it from the probe assembly. Gripping the probe heater as shown, carefully pull it off the probe assembly. Probe heater The probe heater can be contaminated with Warning: biohazardous and/or toxic materials.
Page 174: Fitting The New Ionsabre Ii Probe Heater
Fitting the new IonSABRE II probe heater Required materials • Chemical-resistant, powder-free gloves • IonSABRE II probe heater To fit the new IonSABRE II probe heater: Take great care not to damage the probe heater’s Caution: electrical connections, capillary sleeve, or capillary when fitting the heater over the capillary sleeve.
Page 175: Replacing The Ion Block Source Heater
Replacing the ion block source heater Replace the ion block source heater if it fails to heat when the instrument is pumped down (evacuated). Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool • Needle-nose pliers •...
Page 176 Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw.
Page 177 To avoid damaging the heater cartridge assembly wires, Caution: do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block. Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block.
Page 178 Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and slide the assembly out of the ceramic heater mounting block. Heat-shrink tubing Heater cartridge assembly Ceramic heater mounting block 10. Dispose of the heater cartridge assembly. To avoid damaging the heater cartridge assembly wires, Caution: do not bend or twist them when fitting the assembly to the ceramic...
Page 179: Replacing The Source Assembly Seals
Replacing the source assembly seals To avoid excessive leakage of solvent vapor into the Warning: laboratory atmosphere, the seals listed below must be renewed, at intervals of no greater than 1 year, exactly as described in this section. To avoid excessive leakage of solvent vapor into the laboratory atmosphere, the following seals must be renewed at intervals of no greater than 1 year: •...
Page 180 Probe adjuster nebulizer gas seal Probe adjuster assembly probe seal Use the O-ring removal kit to carefully remove the following seals from the source enclosure: • Source enclosure seal • Nebulizer gas seal • Desolvation gas seal Nebulizer gas seal Desolvation gas seal Source enclosure seal TP03164...
Page 181: Fitting The New Source Enclosure And Probe Adjuster Assembly Probe Seals
The seals can be contaminated with biohazardous Warning: and/or toxic materials. Dispose of them according to local environmental regulations. Dispose of all the seals in accordance with local environmental regulations. Fitting the new source enclosure and probe adjuster assembly probe seals Required materials •...
Page 182: Replacing The Air Filter
Fit the following new seals to the source enclosure: • Nebulizer gas seal • Desolvation gas seal These seals have a special cross-section; fit them in the Requirement: groove as shown. Seal Groove Fit the following new seals to the probe adjuster assembly: •...
Page 183 Unscrew the captive thumbscrew on the filter cover. Thumbscrew Filter cover Remove the filter cover from the instrument. Filter Filter cover Replacing the air filter 4-109...
Page 184: Appi/Apci Source-Changing The Uv Lamp Bulb
Lift the filter, vertically, from the its slot in the instrument. If necessary, use the needle-nose files to grasp the filter. Tip: Dispose of the filter. Fit the new filter into the instrument. Fit the filter cover to the instrument. Tighten the thumbscrew on the filter cover.
Page 185 To change the UV lamp bulb: The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is suitably Warning: prepared before commencing this procedure. Prepare the instrument for working on the source (see page 4-8).
Page 186 To avoid breaking the bulb, do not use a screwdriver to Caution: push the bulb forward in the lamp drive assembly. Insert the length of 4-mm nylon tube through the back of the lamp drive assembly, and push the bulb forward. Nylon tube APPI lamp bulb Remove the bulb from the lamp drive assembly.
Page 187: Appi/Apci Source-Cleaning The Lamp Window
APPI/APCI source—cleaning the lamp window The transmission of the high-energy photons responsible for APPI relies on the cleanliness of the magnesium fluoride lamp window. Clean the window to keep the surface clear of contamination, and thus avoid reduced sensitivity. Required materials •...
Page 188: Appi/Apci Source-Replacing The Appi Lamp Drive Seals
APPI/APCI source—replacing the APPI lamp drive seals To ensure the integrity of the source exhaust system, the Warning: APPI lamp drive assembly O-rings listed below must be renewed at intervals not exceeding one year, exactly as described in this section. The following APPI lamp drive assembly O-rings must be renewed at intervals of no greater than one year: •...
Page 189 To remove the APPI lamp drive assembly seals: The probe and source components can be Warning: contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is in Warning: Standby mode before commencing this procedure.
Page 190 Use the Phillips (cross-head) screwdriver to remove the 2 source enclosure, release-handle screws, and remove the handle. Source enclosure cover screws Lamp-drive cover screws Release handle TP03204 Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the remaining 2 lamp-drive cover screws, which were hidden by the release handle.
Page 191 Lamp mounting flange Mounting-flange screw TP03205 12. Slide the lamp assembly, shaft, and flange out of the APPI source enclosure. The cables remain attached to the shaft, which you fully withdraw Tip: and lay on the bench beside the source enclosure. PEEK insulator Repeller electrode Electrode screw...
Page 192 14. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the two insulator screws. 15. Remove the PEEK insulator from the end of the mounting shaft. 16. Slide the shaft mounting flange off the shaft, and note the correct orientation, for its reassembly.
Page 193 19. Use the O-ring removal kit to carefully remove the shaft mounting flange O-ring from the APPI source enclosure side. Lamp mounting flange O-ring The O-rings can be contaminated with Warning: biohazardous and/or toxic materials. Ensure that they are correctly disposed of according to local environmental regulations.
Page 194: Fitting The New Appi Lamp Drive Assembly O-Rings
Fitting the new APPI lamp drive assembly O-rings Required materials • Chemical-resistant, powder-free gloves • 3-mm Allen wrench. • Small Phillips (cross-head) screwdriver. • Small Pozidriv screwdriver. • 1:1 methanol/water • Lint-free cloth To fit the new APPI lamp drive assembly O-rings: The lamp drive assembly components can be Warning: contaminated with biohazardous and/or toxic materials.
Page 195 Fit the mounting shaft insertion tool to the mounting shaft. Mounting shaft insertion tool To prevent damage to the mounting shaft O-rings, fit the Caution: mounting shaft insertion tool to the mounting shaft before fitting the shaft to the lamp mounting flange. Slide the lamp mounting flange onto the shaft, taking care to align it correctly.
Page 196 12. Fit the repeller electrode to the PEEK insulator. 13. Use the small Phillips screwdriver to fit and tighten the repeller electrode retaining screw. 14. Insert the UV bulb into the lamp drive assembly and push it fully home. 15. Fully retract the lamp mounting shaft from the source enclosure. 16.
Page 197 This appendix presents all the safety symbols and statements that apply to the entire line of Waters products. Contents Topic...
Page 198: A Safety Advisories
Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.
Page 199: Specific Warnings
The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments fitted with nonmetallic tubing. Pressurized nonmetallic, or polymer, tubing can burst. Warning: Observe these precautions when working around such tubing: •...
Page 200 Biohazard warning This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.
Page 201: Caution Symbol
Chemical hazard warning This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material. Waters instruments can be used to analyze or Warning: process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety...
Page 202: Warnings That Apply To All Waters Instruments
Warnings that apply to all Waters instruments When operating this device, follow standard quality control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
Page 203 • Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. • Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. • Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. Warnings that apply to all Waters instruments...
Page 204 Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
Page 205 농축 질산 또는 황산과 함께 사용하지 마십시오. • 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오. 警告：圧力のかかったポリマーチューブを扱うときは、注意してください。 • 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。 • 近くにある火を消してください。 • 著しく変形した、または折れ曲がったチューブは使用しないでください。 • 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流さないでください。 • 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧力で破裂します。 Warnings that apply to all Waters instruments...
Page 206 Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
Page 207: Electrical And Handling Symbols
Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse Recycle symbol: Do not dispose in municipal waste.
Page 208: Handling Symbols
Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks! A-12 Safety Advisories...
Page 209 Mass spectrometer external wiring and vacuum connections Connecting the Oerlikon Leybold oil-filled roughing pumps Connecting the Edwards oil-free roughing pumps B-10 Making the roughing pump connections to the Xevo TQ-S B-16 Connecting to the nitrogen supply B-17 Connecting to the collision cell gas supply...
Page 210: B External Connections
Mass spectrometer external wiring and vacuum connections The instrument’s rear panel connectors are shown below. Connectors and controls not identified in the following figure are for Note: Waters engineers use only. Mass spectrometer rear panel connectors: Shielded Ethernet LA N External Connections 1...
Page 211: Connecting The Oerlikon Leybold Oil-Filled Roughing Pumps
Connecting the Oerlikon Leybold oil-filled roughing pumps ® This option requires the use of two, identical, Oerlikon Leybold™, oil-filled roughing pumps. To connect the alternative, oil-free roughing pumps, see page B-10. Note: Oerlikon Leybold oil-filled roughing pump: Electrical connections Inlet port Exhaust port TP03296 Connecting the Oerlikon Leybold oil-filled roughing pumps...
Page 212: Making Tubing Connections
Making tubing connections Required materials • Chemical-resistant, powder-free gloves • 7-mm nut driver • Sharp knife ® The following items are included in the Xevo TQ-S installation kit: • NW40 flanged flexible tubing, 1-m long • NW40 hose assembly, 1.5-m long •...
Page 213 To make the tubing connections: The pumps and their connections can be contaminated Warning: with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves when performing this procedure. Caution: • To ensure correct operation of the roughing pumps, install each pump within 2 degrees of horizontal.
Page 214 The roughing pumps are heavy. To avoid injury, at least Warning: two people must lift each pump. Place the pumps on the PTFE drip tray, facing the same orientation. To Xevo TQ-S source vacuum port To Xevo TQ-S turbo NW40 flanged NW40 hose...
Page 215 NW40 clamp, tightening it with the 7-mm nut driver. Make the connections between the NW25 and NW40 hose assemblies and the vacuum ports on the rear of the Xevo TQ-S (see page B-16). To avoid gas leaks, use the sharp knife to cut the PVC...
Page 216 The instrument requires two separate exhaust systems: Caution: one for nitrogen, the other for the roughing pump. Vent them to atmosphere through separate exhaust lines. Oil mist can seriously damage the instrument if the nitrogen exhaust line connects with the roughing pump exhaust line. Your warranty does not cover damage caused by routing exhaust lines incorrectly.
Page 217 LA N External Connections 1 Service Bus EPC Com Port External Connections 2 Video Output OUT - External Connections 1 External Connections 2 Auxiliary 10MB Activity /100MB Roughing pump number 2 Top roughing pump connector Roughing pump number 1 Roughing pump d.c.
Page 218: Connecting The Edwards Oil-Free Roughing Pumps
Connecting the Edwards oil-free roughing pumps This option requires the use of two, Edwards, oil-free roughing pumps (types XDS46i and XDS100B) as an alternative to the oil-filled roughing pumps. To connect the oil-filled roughing pumps, see “Connecting the Oerlikon Note: Leybold oil-filled roughing pumps”...
Page 219 Required materials • Chemical-resistant, powder-free gloves • 7-mm nut driver The following items are included in the Xevo TQ-S installation kit: • NW40 flanged flexible tubing, 1 m long • NW40 hose assembly, 1.5 m long • NW25 hose assembly, 1.5 m long •...
Page 220 To connect the oil-free roughing pumps: The pumps and connections can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves when performing this procedure. The roughing pumps are heavy. To avoid injury, at least two Warning: people must lift each pump.
Page 221 To Xevo TQ-S source vacuum port To Xevo TQ-S turbo vacuum port NW40 hose NW40 flanged assembly flexible tubing NW25 hose assembly NW25/NW40 adaptor NW40 tee To exhaust vent XDS100B pump XDS46i pump PVC exhaust tubing Using an NW40 center ring, attach one end of the 1-m length of NW40...
Page 222 XDS100B pump, and then secure the connection with an NW40 clamp, tightening it with the 7-mm nut driver. Make the connections between the NW25 and NW40 hose assemblies and the vacuum ports on the rear of the Xevo TQ-S (see page B-16).
Page 223 LA N External Connections 1 Service Bus EPC Com Port External Connections 2 Video Output OUT - External Connections 1 External Connections 2 Auxiliary 10MB Activity /100MB Roughing pump connectors XDS100B pump XDS46i pump Roughing pump main power connector Roughing pump d.c.
Page 224: Making The Roughing Pump Connections To The Xevo Tq-S
Using an NW25 center ring, attach the NW25 elbow to the turbo vacuum port of the Xevo TQ-S, and then secure the connection with an NW25 clamp, tightening it with the 7-mm nut driver, see the figure on page B-17.
Page 225: Connecting To The Nitrogen Supply
Connecting to the nitrogen supply Required materials • Chemical-resistant, powder-free gloves • 6-mm PTFE tubing (included in the Waters Installation Kit) • Nitrogen regulator To connect the nitrogen supply: Connect one end of the 6-mm PTFE tubing to the nitrogen inlet port on the rear of the instrument.
Page 226: Connecting To The Collision Cell Gas Supply
Connecting to the collision cell gas supply Required materials • Chemical-resistant, powder-free gloves • 11-mm wrench ® • 1/8-inch Swagelok nut and ferrule • 1/8-inch stainless steel tube (supplied with the mass spectrometer) • Argon regulator (not supplied) To connect the collision cell gas supply: Use the 1/8-inch Swagelok nut and ferrule to connect the 1/8-inch stainless steel tube to the collision cell gas inlet on the rear of the mass spectrometer (see the figure on...
Page 227: Connecting The Nitrogen Exhaust Line
Connecting the nitrogen exhaust line Required materials • Chemical-resistant, powder-free gloves • Sharp knife • Nitrogen exhaust trap bottle • 4-mm and 12-mm PTFE tubing (included in the installation kit) To connect the nitrogen exhaust line: Warning: • Biohazardous and/or toxic LC solvents and analytes can be carried in the nitrogen exhaust, which must be vented via the nitrogen exhaust trap bottle and laboratory exhaust system.
Page 228 Nitrogen exhaust trap bottle: To laboratory exhaust port From instrument exhaust connection From instrument pilot valve port Nitrogen exhaust trap bottle Bottle support To avoid gas leaks, use the sharp knife to cut the PTFE Caution: tubing squarely (that is, perpendicular to its horizontal axis). Cut a length of 4-mm tubing long enough to connect the instrument to the nitrogen exhaust trap bottle.
Page 229 Connect one end of the tubing to the exhaust port on the instrument’s rear panel. Connect the free end of the tubing to the inlet port on the nitrogen exhaust trap bottle. Requirement: Ensure that the tubing has no loops and does not sag. To avoid gas leaks, use the sharp knife to cut the PTFE Caution: tubing squarely (that is, perpendicular to its horizontal axis).
Page 230: Connecting The Liquid Waste Line
Connecting the liquid waste line Required materials • Chemical-resistant, powder-free gloves • Waste container To connect the liquid waste line: The waste line and connection can be contaminated with Warning: biologically hazardous materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Place a suitable waste container below the mass spectrometer.
Page 231 To prevent leakage of biologically hazardous Warning: materials, • do not crimp or bend drain line. A crimp or bend can impede flow to the waste container. • empty the waste container before the lower end of the drain tube is covered by waste solvent. Route the waste line to the waste container.
Page 232: Connecting The Workstation
Connecting the workstation Before connecting the workstation to the instrument, set up the workstation according to its accompanying instructions. Locate the workstation within 5 meters of the instrument. Use shielded network cables with the mass spectrometer to Requirement: ensure compliance with FCC limits. To connect the workstation: Connect the monitor to the PC.
Page 233: Connecting Ethernet Cables
Connecting Ethernet cables Use shielded network cables with the mass spectrometer to Requirement: ensure compliance with FCC limits. To make Ethernet connections: Connect one end of one shielded Ethernet cable to the network switch, and then connect the other end to the Ethernet card on the ®...
Page 234 Input and output connector locations: LA N External Connections 1 Service Bus External connections identification tables EPC Com Port External Connections 2 Video Output OUT - External connections 1 External Connections 1 External connections 2 External Connections 2 System Activity Input/output signal connector configuration: B-26 External Connections...
Page 235 External connections 1: Function Rating Event In 1+, digital signal, optimum +3.3V +5 V max +5V Event In 1-, digital ground, 0V Not used Event In 2+, digital signal, optimum +3.3V +5 V max +5V Event In 2-, digital ground, 0V Not used Not used CE Interlock Out, common...
Page 236: Making Signal Connections
Making signal connections Mass spectrometer signal connections: Signal connections Description Analog (Out) Used for analog chart output functionality. The output voltage range is 0 to 1 V. The resolution of the voltage output is 12 bits. Gas Fail Interlock Used to stop the solvent flow if the nitrogen gas (Out) supply fails.
Page 237 Attach the positive and negative leads of the signal cable to the connector. Connector Signal cable TP02585 Slide the clamp (with the bend facing down) into the protective shield. Insert the clamp and shield (with the bend facing down) into the connection cover, and loosely tighten with one self-tapping screw.
Page 238 Insert the connector with the signal cable into the connection cover, position the clamp over the cable leads, and tighten the clamp into place with the second self-tapping screw. Cable leads Clamp TP02587 Place the second connection cover over the first cover, and snap it into place.
Page 239: Connecting To The Electricity Source
To avoid electric shock, use the SVT-type power cord in the Warning: United States and HAR-type (or better) in Europe. For information regarding what cord to use in other countries, contact your local Waters distributor. Connect the female end of the power cord to the receptacle on the rear panel of the mass spectrometer.
Page 240 B-32 External Connections...
Page 241 Materials of Construction and Compatible Solvents To confirm the integrity of the source exhaust Warning: system, you must address any safety issues raised by the contents of this Appendix. Contents Topic Page Preventing contamination Items exposed to solvent Solvents used to prepare mobile phases...
Page 242: C Materials Of Construction And Compatible Solvents
Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). Visit www.waters.com. Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents typically used with these items.
Page 243: Solvents Used To Prepare Mobile Phases
Items exposed to solvent: (Continued) Item Material Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel, polybutylene terephthalate, and polyoxymethylene APPI lamp drive assembly: Mounting shaft Stainless steel Repeller electrode Stainless steel Insulator PEEK Lamp window Magnesium fluoride Solvents used to prepare mobile phases These solvents are the most common ingredients used to prepare mobile phases for reverse-phase LC/MS (API): •...
Page 244 Materials of Construction and Compatible Solvents...
Page 245 Plumbing the IntelliStart Fluidics system This appendix provides reference information for replacing the tubing in the IntelliStart™ Fluidics system. The IntelliStart Fluidics components can be Warning: contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while working on the system. Contents Topic Page...
Page 246: D Plumbing The Intellistart Fluidics System
Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). You can find this document on http://www.waters.com; click Services and Support > Support. Plumbing schematic Selector valve Diverter valve Vial A Vial B...
Page 247 Tubing specifications The following table gives the internal diameter (ID), external diameter (ED), color, length, and quantity for the IntelliStart Fluidics tubing. Replacement tubing specifications: Length Connection Color Quantity (inches) (inches) (mm) Select to 0.005 1/16 divert Vial 0.020 1/16 Orange Wash 0.020...
Page 248 Plumbing the IntelliStart Fluidics system...
Page 249: Index
Index acquisition speed biohazard warning air filter 4-108 burst warning analog signal connection B-28 APCI cables corona pin 3-12 network B-24 defined source ionization mode APPI/APCI 3-16 IonSABRE II probe capillary, replacing 4-91 NanoFlow 3-21 heater, replacing 4-98 calibrating viii removing 3-10 caution symbol...
Page 250 3-21 probe workstation B-24 capillary, replacing 4-82 Connections Insight installing constant neutral loss mode 1-17 removing contacting Waters tip, replacing 4-78 contamination, preventing Ethernet cables, connecting B-25 corona pin event in signal connection B-28 APCI 3-12 event out signal connection...
Page 251 operation mode, changing overview gas ballasting power LED Edwards XDS46i roughing pump rear panel connections 1-19 4-36 rebooting Oerlikon Leybold roughing pumps source, preparing for working on 4-23 gas fail interlock connection B-28 specifications starting handling symbols A-12 tuning handling waste liquid 4-21 vacuum connections hazards...
Page 252 MS operating modes 1-13 power MS/MS operating modes 1-14 liquid waste line, connecting B-22 multiple reaction monitoring mode 1-16 maintenance procedures nanoACQUITY Xevo TQ-S UPLC/MS safety system schedule NanoFlow mass spectrometer ESI source calibrating glass capillary case, cleaning 4-20 electrospray, restarting...
Page 253 opening source isolation valve 4-18 probes operate LED APCI operating modes ASAP APPI 3-11 constant neutral loss mode 1-17 installing daughter 1-15 removing full scan 1-13 IonSABRE II 1-16 product ion mode 1-15 1-13 pumps MS/MS 1-14 roughing 1-18 multiple reaction monitoring 1-16 Edwards B-10...
Page 254 IonSABRE II probe solvents capillary 4-91 exposure of components to heater 4-98 used in mobile phases Oerlikon Leybold roughing pump source demister element 4-27 assembly seals, replacing 4-105 4-27 components, cleaning 4-37 source assembly seals 4-105 enclosure release reservoirs, IntelliStart Fluidics system heater, replacing 4-101 isolation valve...
Page 255 TRIZAIC UPLC source 1-10 Xevo TQ-S, removing from service troubleshooting with Connections Insight ZSpray source tuning turbomolecular pump 1-18 T-Wave/ScanWave collision cell 1-12 Universal sprayer, fitting 3-20 UV lamp cleaning window 4-113 replacing bulb 4-111 turning on 3-14 vacuum connections...
Page 256 Index-8...

Waters Xevo TQ-S Operator's, Overview And Maintenance Manual

Copyright Notice

Trademarks

Customer Comments

Safety Considerations

Operating this Instrument

ISM Classification

EC Authorized Representative

1 Waters Xevo TQ-S Overview

Waters Xevo TQ-S

Ionization Techniques and Source Probes

Intellistart Fluidics System

Ion Optics

MS Operating Modes

MS/MS Operating Modes

Leak Sensors

Vacuum System

Rear Panel

2 Preparing the Mass Spectrometer for Operation

Starting the Mass Spectrometer

Preparing the Intellistart Fluidics System

Rebooting the Mass Spectrometer

Leaving the Mass Spectrometer Ready for Operation

Emergency Shutdown of the Mass Spectrometer

3 Changing the Mode of Operation

ESI Mode

Esci Mode

APCI Mode

Combined APPI/APCI Source

Nanoflow ESI Source

4 Maintenance Procedures

Maintenance Schedule

Spare Parts

Troubleshooting with Connections INSIGHT

Safety and Handling

Preparing the Instrument for Working on the Source

Removal and Refitting of the Source Enclosure

Installing and Removing the Corona Pin

Operating the Source Isolation Valve

Removing O-Rings and Seals

Cleaning the Instrument Case

Emptying the Nitrogen Exhaust Trap Bottle

Maintaining the Oerlikon Leybold Oil-Filled Roughing Pumps

Gas Ballasting the Edwards Xds46I Oil-Free Roughing Pump

Cleaning the Source Components

Cleaning the Sampling Cone Assembly

Cleaning the Ion Block Assembly

Cleaning the Stepwave Ion Guide Assembly

Replacing the ESI Probe Tip and Gasket

Replacing the ESI Probe Sample Capillary

Cleaning the Ionsabre II Probe Tip

Replacing the Ionsabre II Probe Sample Capillary

Cleaning or Replacing the Corona Pin

Replacing the Ionsabre II Probe Heater

Replacing the Ion Block Source Heater

Replacing the Source Assembly Seals

Replacing the Air Filter

APPI/APCI Source-Changing the UV Lamp Bulb

APPI/APCI Source-Cleaning the Lamp Window

APPI/APCI Source-Replacing the APPI Lamp Drive Seals

A Safety Advisories

Warning Symbols

Caution Symbol

Warnings that Apply to All Waters Instruments

Electrical and Handling Symbols

B External Connections

Mass Spectrometer External Wiring and Vacuum Connections

Connecting the Oerlikon Leybold Oil-Filled Roughing Pumps

Connecting the Edwards Oil-Free Roughing Pumps

Making the Roughing Pump Connections to the Xevo TQ-S

Connecting to the Nitrogen Supply

Connecting to the Collision Cell Gas Supply

Connecting the Nitrogen Exhaust Line

Connecting the Liquid Waste Line

Connecting the Workstation

Connecting Ethernet Cables

Input/Output Signal Connectors

Connecting to the Electricity Source

C Materials of Construction and Compatible Solvents

Preventing Contamination