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1.8.2 SIR mode
Use the selected ion recording (SIR) mode for quantitation when you cannot find a suitable
fragment ion to perform a more specific multiple reaction monitoring (MRM) analysis (see
mode
for further details). In SIR and MRM modes, neither quadrupole is scanned, and no
spectrum (intensity versus mass) is produced. The data obtained from SIR or MRM analyses
derive from the chromatogram plot—specified mass intensity (SIR) or specified transition (MRM)
versus time.
1.8.3 MS1 scanning mode
In MS1 scanning mode, the instrument can acquire data at scan speeds as high as 2,000 Da/s.
Use this mode for reduced fragmentation. This scanning mode cannot switch between scanning
and SIR/MRM in the same acquisition. See the mass spectrometer's online help for further
information.
1.9 MS/MS operating modes
The following table shows the MS/MS operating modes.
Table 1–5: MS/MS operating modes:
Operating mode
Product (daughter) ion
spectrum
Precursor (parent) ion
spectrum
MRM
PICS (Product Ion
Confirmation Scan)
RADAR is an additional mode that allows you to create experiments containing both MRM and
SIR functions together with MS scan.
1.9.1 Product (daughter) ion spectrum
Product ion spectrum is the most commonly used MS/MS operating mode. You can specify an ion
of interest for fragmentation in the collision cell, yielding structural information.
MS1
Static (at precursor
mass)
Scanning
Static (at precursor
mass)
Static (at precursor
mass)
April 27, 2020, 715005564 Ver. 01 (previously released as Rev. A)
Collision cell
Fragment precursor
ions and pass all
masses
Page 31
MRM
MS2
Scanning
Static (at product
mass)
Static (at product
mass)
Switching between
static (at product
mass) and scanning
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