Multiple Reaction Monitoring Mode - Waters Xevo TQ MS Operator's, Overview And Maintenance Manual

Typical application
You typically use the precursor ion mode for structural elucidation—that is, to
complement or confirm product scan data—by scanning for all the precursors
of a common product ion.

Multiple reaction monitoring mode

Multiple reaction monitoring (MRM) mode is a highly selective MS/MS
equivalent of SIR. Because both MS1 and MS2 are static, greater dwell time
on the ions of interest is possible and so the sensitivity achieved is better
compared with scanning-mode MS/MS. This mode is the most commonly used
acquisition mode for quantitative analysis, allowing the compound of interest
to be isolated from the chemical background noise.
Multiple reaction monitoring mode:
Typical application
You typically use MRM mode to quantify known analytes in complex samples:
• Drug metabolite and pharmacokinetic studies.
• Environmental, for example, pesticide and herbicide analysis.
• Forensic or toxicology, for example, screening for target drugs in sport.
MRM mode does not produce a spectrum, because only one transition is
monitored at a time. As in SIR mode, a chromatogram is produced.
MS1
Static (at precursor mass)
Collision cell
Pass all masses
February 11, 2013, 71500130002 Rev. D
MS/MS operating modes
MS2
Static (at product mass)
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