Product (daughter) ion mode
Product ion mode is the most commonly used MS/MS operating mode. You can
specify an ion of interest for fragmentation in the collision cell, thus yielding
structural information.
Product ion mode:
Typical applications
You typically use product ion mode for the following applications:
•
Method development for MRM screening studies:
–
Identifying product ions for use in MRM transitions.
–
Optimizing CID tuning conditions to maximize the yield of a specific
product ion to be used in MRM analysis.
•
Structural elucidation (for example, peptide sequencing).
MS1
Static (at precursor mass)
Collision cell
Pass all masses
MS/MS operating modes
MS2
Scanning
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