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Verifying the installation and setup
Performing a mock run
Performing a mock run
Before you start
Transferring the cartridges to the 96AM Cartridge & Tip Seating Station
90
To verify that the profile is configured and taught correctly for the installed
hardware, you should perform a mock run of the Affinity Purification protocol.
For the mock run, you use the AssayMAP Resin- Free cartridges instead of
packed cartridges, and you do not use any labware on the Bravo deck.
Ensure that you have the required materials.
Table Required materials
Item
AssayMAP Resin- Free cartridge rack
IMPORTANT
The protocol will not run if the
cartridges are missing.
IMPORTANT
Do not use the resin- free cartridges from
the AssayMAP Syringe Test Kit for the mock run. Any
potential damage to these cartridges during the mock
run would render them inappropriate for the syringe
test. Using damaged resin- free cartridges for the
AssayMAP syringe test could impact the test results.
Deionized water in the source bottle for the Pump
Module
The cartridges must be in the Cartridge & Tip Seating Station before you run
the Affinity Purification protocol. You can use the Cartridge Transfer utility to
move full columns of AssayMAP Cartridges from the source cartridge rack
(96AM Cartridge Rack and Receiver Plate) to the destination cartridge rack
(Cartridge & Tip Seating Station).
Before you start
Make sure the Bravo deck is set up as follows:
•
The empty Cartridge & Tip Seating Station is at deck location 2.
•
The 96AM Cartridge Rack and Receiver Plate is at deck location 6 and
contains 12 full columns of 8 cartridges each. Make sure you remove the
lid.
•
The 96AM Wash Station is at deck location 1.
•
All other deck locations are empty.
Part number
Agilent
G5496- 60009
Not applicable
AssayMAP Bravo Platform Installation Guide
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