Agilent Technologies AssayMAP Bravo Installation Manual page 100

Verifying the installation and setup
Performing a mock run
Performing a mock run of the Affinity Purification protocol
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2
In the A. Application Settings area, specify the following settings for the run:
Setting
First Column in Source
Cartridge Rack
Number of Columns to Transfer 12
First Column in Destination
Cartridge Rack
3
Confirm that the physical layout on the AssayMAP Bravo deck matches the
Deck Layout and Labware Table sections of the interface.
4
Ensure that the labware are properly seated in the platepads.
CAUTION
Improperly seated labware can cause a hardware collision, resulting in
equipment damage. Ensure that all labware are properly seated within the alignment
features of their respective platepads.
5
Click Run Cartridge Transfer to start the run.
To monitor the progress of the run, check the Status box in the upper right
corner of the form.
6
When the run is finished, remove the 96AM Cartridge Rack and Receiver
Plate from deck location 6.
IMPORTANT
For this mock run, you will not use any additional labware.
Ensure that the only items on the deck are the 96AM Wash Station at deck
location 1 and the Cartridge & Tip Seating Station containing the Resin- Free
cartridges at deck location 2.
To perform a mock run of the Affinity Purification protocol:
1
In the Protein Sample Prep Workbench, open the App Library and locate Affinity
Purification. Click App to open the application.
The Affinity Purification application opens.
Value
1
1
AssayMAP Bravo Platform Installation Guide