Agilent Technologies AssayMAP Bravo Installation Manual page 103

Table Automation movements during the Affinity Purification protocol
Protocol step
Preliminary check
Initial Syringe Wash
Prime
Equilibrate
Load Samples
Cup Wash 1
AssayMAP Bravo Platform Installation Guide
Head moves to Bravo Platform action
deck location...
2 Checks the head and parks any cartridges that may have been
mounted on the head from a protocol that had been
previously aborted.
1 Dispenses any liquid remaining in the syringes into the wash
station.
1 Washes the syringes the specified number of times.
3 Aspirates the Priming Buffer.
2 Mounts the cartridges on the head.
1 Dispenses the Priming Buffer through the cartridges into the
wash station.
2 Parks the cartridges in the seating station.
1 Washes the syringes at the wash station.
3 Aspirates the Equilibration Buffer.
2 Mounts the cartridges on the head.
1 Dispenses the Equilibration Buffer through the cartridges to
equilibrate.
2 Parks the cartridges in the seating station.
1 Washes the syringes at the wash station.
4 Aspirates the samples into the syringes.
1 Washes the exterior of the syringe probes at the wash station.
2 Mounts the cartridges on the head.
7 Dispenses sample through the cartridges to load sample.
Collect flow through in the Flow Through Collection plate.
1 Washes the cartridge exteriors at the wash station.
2 Parks the cartridges at the seating station.
1 Washes the syringes at the wash station.
5 Aspirates the Cartridge Wash Buffer 1 into the syringes.
2 Performs a Cup Wash.
1 Dispenses buffer at the wash station.
1 Washes the syringes at the wash station.
Verifying the installation and setup
Performing a mock run
95