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Verifying the installation and setup
Washing the syringes to prepare for first time use
Washing the syringes to prepare for first time use
Before you start
86
5
To recover from the emergency stop:
a
At the emergency- stop pendant, turn the red button clockwise. The
spring- loaded button pops up.
b
In the AssayMAP Bravo Error dialog box, click Abort to end the protocol.
c
Run the Startup utility again to reset the system. For details, see
"Running the Startup utility" on page
6
Click Run Cartridge Transfer to start another run.
7
As soon as the Bravo head starts to move away from its position above the
wash station and towards deck location 6, use a pencil to interrupt the
Light Curtain.
The Bravo head stops moving and an error message appears.
8
To recover from the emergency stop:
a
Ensure that no objects are in the path of the Light Curtain.
b
In the AssayMAP Bravo Error dialog box, click Abort to end the protocol.
c
Run the Startup utility again to reset the system. For details, see
"Running the Startup utility" on page
Use the following procedure to prepare the new syringes for use. The
procedure primes and washes the syringes for 60 cycles.
Ensure that you have the following required materials.
Item
Name (manufacturer and part number)
One
Use one of the following labware:
•
labware
Reservoir, Seahorse 201254- 100, PP, no walls, pyramid
for wash
bottom (Agilent 201254- 100)
buffer
•
12- Column Low- Profile Reservoir (Agilent 201280- 100)
•
8- Row Low- Profile PolyPro Reservoir (Agilent 201282- 100)
Deionized
Not applicable
water
83.
83.
AssayMAP Bravo Platform Installation Guide
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