Agilent Technologies AssayMAP Bravo Installation Manual page 69

Fine tuning the teachpoints
AssayMAP Bravo Platform Installation Guide
a
Verify that the A1 pipette tip is perfectly centered above the platepad
crosshairs in the x- and y- axes.
b
Set the Jog Axes Up/Down increment to 0.2- mm. Carefully, jog the head
down in 0.2- mm increments just until the feeler gauge cannot fit under
the pipette tip.
c
Raise the head by 0.05- mm increments until you reach the lowest
possible height that allows the 0.010- inch feeler gauge to slide under
the pipette tip without deflecting it.
6
Click Teach.
7
To derive the teachpoints for the remaining locations from deck location 2:
a
In the Advanced operations list, select Set all teachpoints based upon the
selected teachpoint.
b
Ensure that deck location 2 is still selected, and then click the Perform
advanced operations.
c
Click OK in the confirmation message.
To fine tune the automatically derived teachpoints:
1
In the Jog/Teach tab image of the Bravo deck, click Location 3.
2
In the Approach height above teachpoint list, select 10, and then click Approach.
The Bravo head moves to deck location 3 and moves down so that the
pipette tip is approximately 10 mm above the platepad crosshairs.
3
Carefully, use the Jog Axes controls to adjust the position of the A1 pipette
tip above the crosshairs. Use incrementally smaller jog increments as you
jog the tip down closer to the crosshairs.
4
When the pipette tip is 1–2 mm above the crosshairs, do the following:
a
Verify that the A1 pipette tip is perfectly centered above the crosshairs
in the x- and y- axes.
b
Set the Jog Axes Up/Down increment to 0.2- mm. Carefully, jog the head
down in 0.2- mm increments just until the feeler gauge cannot fit under
the pipette tip.
c
Raise the head by 0.05- mm increments until you reach the lowest
possible height that allows the 0.010- inch feeler gauge to slide under
the pipette tip without deflecting it.
5
Click Teach.
6
Repeat steps 1 to 5 for deck locations 4, 5, 6, 7, and 8.
IMPORTANT
If the Peltier Thermal Station is installed at deck
location 4, ensure that the plate nest is empty and the PCR plate insert
is not installed.
7
Adjust the teachpoints for the accessory locations. See
Adjusting teachpoints for accessories" on page
Teaching part 1: Setting teachpoints in AssayMAP 1 profile
62.
Setting the teachpoints
"Teaching part 2:
61