Waters ACQUITY UPLC Manuals

Manuals and User Guides for Waters ACQUITY UPLC. We have 6 Waters ACQUITY UPLC manuals available for free PDF download: Operator's Manual, Operator's, Overview And Maintenance Manual, Overview And Maintenance Manual, Care And Use Manual

Waters ACQUITY UPLC Operator's Manual (514 pages)

Brand: Waters | Category: Laboratory Equipment | Size: 6 MB

Copyright Notice
2
Trademarks
2
Customer Comments
3
Contacting Waters
3
Safety Considerations
4
Considerations Specific to ACQUITY UPLC Instruments
4
Safety Advisories
4
Operating the ACQUITY UPLC Instruments
5
Applicable Symbols
5
Audience and Purpose
5
Intended Use of the ACQUITY UPLC System
5
Calibrating
5
Quality-Control
6
ISM Classification
6
ISM Classification: ISM Group 1 Class B
6
EC Authorized Representative
6
Table of Contents
7
System Overview
19
Instruments, Components, and Data Systems
20
Examples of Waters ACQUITY UPLC Systems
21
UPLC System Guidelines
22
ACQUITY UPLC Columns Calculator
23
Binary Solvent Manager
23
How the Binary Solvent Manager Works
24
Sample Manager
25
How Sample Flows
25
High Temperature Column Heater
26
Column Manager
26
Column Heater/Cooler
27
30-CM Column Heater/Cooler
27
Optional Sample Organizer
27
Detectors
28
TUV Detector
28
PDA Detector
28
ELS Detector
28
FLR Detector
29
Median Baseline Filter
29
Mass Spectrometers
29
SQ Detector
29
TQ Detector
29
Data Systems
30
Empower Software
30
Masslynx Software
30
Columns
30
Ecord Column Chip
31
Flexcart
31
For Additional Information
32
System Setup
33
Before You Begin
34
Tools
35
Assembling the Flexcart
35
Adjusting the Flexcart's Height
38
Locking the Flexcart in Place
39
Moving the Assembled Flexcart
41
Unpacking and Installing the Sample Organizer
41
Installing the Binary Solvent Manager
42
Installing the Sample Manager
42
Installing the HT Column Heater
43
Required Materials
43
Installing the Optional Column Manager
44
Installing the Optional Column Heater/Cooler
45
Installing the Detector
45
Installing the 30-CM Column Heater/Cooler
46
Plumbing the System
52
Installation Recommendations for Fittings
53
Plumbing a TUV Detector
54
Plumbing a PDA Detector
57
Plumbing an ELS Detector
57
Plumbing an FLR Detector
57
Plumbing the Binary Solvent Manager and Sample Manager
58
Plumbing the Column Manager
65
Plumbing the 30-CM Column Heater/Cooler
66
Routing System Drainage through the Flexcart
68
Installing the Column Stabilizer Assembly in an HT Column Heater
69
Required Materials
69
Installing the Column Stabilizer Assembly in a Column Heater/Cooler
72
Installing the Column In-Line Filter in a HT Column Heater
75
Installing the Column In-Line Filter in a Column Manager or Column Heater/Cooler
76
Installing the Column in an HT Column Heater
78
Installing the Column in a Column Manager or Column Heater/Cooler
81
Installing the Column in a System with a Mass Spectrometer
85
Connecting to the Solvent Supply
87
Making Ethernet and Signal Connections
89
Ethernet Connections
89
HT Column Heater Connections
89
30-CM Column Heater/Cooler Connections
90
Binary Solvent Manager I/O Signal Connectors
91
Sample Manager I/O Signal Connectors
93
TUV Detector I/O Signal Connectors
95
PDA Detector Signal Connectors
96
ELS Detector Signal Connectors
96
FLR Detector Signal Connectors
96
Signal Connections
96
Connecting to the Electricity Source
97
Calibrating the XYZ Mechanism Using the Teach Block
99
Preparing System Hardware
105
Powering on the System
105
Monitoring Startup Tests
107
Monitoring System Instrument Leds
107
Power LED
108
Status Leds
108
Enabling the Leak Sensors
110
Preparing the Binary Solvent Manager
111
Performing a Seal Wash Prime
111
Required Materials
112
Priming the Binary Solvent Manager
113
Priming a Dry Binary Solvent Manager
114
Priming a Wetted Binary Solvent Manager
116
Preparing the Sample Manager
119
Selecting Weak Wash and Strong Wash Solvents
119
Priming the Sample Manager
121
Washing the Sample Manager Needle
123
Characterizing the Needle Seal
125
Characterizing the Needle and Sample Loop Volumes
126
Using the Extended Puncture Needle
127
Loading Sample Plates in the Sample Manager
128
Selecting the Optimum Sample Injection Mode
129
Installing the Optional Sample Manager Shade
132
Preparing the Column Manager
134
Preparing the Sample Organizer
134
Initiating Communications
134
Loading Sample Plates
135
Displaying Sample Plate Information
139
Starting the TUV Detector
140
Conditioning the Column
143
Shutting down the System
144
Shutting down for Less than 24 Hours
144
Shutting down for more than 24 Hours
145
Running HPLC Methods on an ACQUITY UPLC System
146
System Considerations
146
Choosing Fittings
148
Configuring System Software
151
Configuring Empower Software
151
Starting Empower Software and Logging in
151
Selecting System Instruments
152
About the Binary Solvent Manager Control Panel
154
About the Sample Manager Control Panel
156
About the TUV Detector Control Panel
159
About the Column Manager Control Panel
161
Starting the ACQUITY UPLC Console from Empower Software
162
Configuring Masslynx Software
163
Starting the ACQUITY UPLC Console from Masslynx Software
164
Verifying System Operation
165
Preparing the System
166
Creating the Test Methods
169
Creating the Instrument Method
169
Creating the Sample Set Method
173
Performing the Gradient Performance Test
174
Maintaining the System
177
Maintenance Schedule
178
Spare Parts
181
Contacting Waters Technical Service
182
Locating System Serial Numbers
182
Maintenance Considerations
183
Safety and Handling
183
Proper Operating Procedures
184
Configuring Maintenance Warnings
184
Maintaining the Binary Solvent Manager
185
Resolving Binary Solvent Manager Leak Sensor Errors
186
Required Materials
187
Replacing the Binary Solvent Manager's Leak Sensor
191
Replacing the Mixer
195
Replacing the I Valve Actuator
197
Replacing the I Valve Cartridge
208
Replacing the In-Line Filter Cartridge on the I Valve Actuator
218
Replacing the Accumulator Check Valve
222
Required Materials
222
Replacing Solvent Filters
225
Cleaning the Air Filters in the Binary Solvent Manager Door
226
Replacing the Air Filters in the Binary Solvent Manager Door
227
Removing and Replacing the Primary Head Seals
228
Removing and Replacing the Accumulator Head Seals
246
Required Materials
246
Replacing the Primary Head Plungers
259
Required Materials
259
Replacing the Accumulator Head Plungers
279
Required Materials
279
Replacing the Vent Valve Cartridge
293
Required Materials
293
Replacing the Fuses
296
Cleaning the Instrument's Exterior
298
Maintaining the Sample Manager
298
Defrosting the Sample Compartment
298
Resolving Sample Manager Leak Sensor Errors
299
Replacing the Sample Manager's Leak Sensor
304
Replacing the Sample Needle Assembly
307
Calibrating the Needle Z-Axis
315
Characterizing the Needle Seal
316
Characterizing the Needle and Sample Loop Volumes
316
Replacing the Puncture Needle Holder
318
Required Materials
318
Replacing the Sample Loop
321
Replacing the Sample Syringe
325
Replacing the Wash Syringes
328
Modifying Sample Syringe Configuration Parameters
331
Replacing the Injection Valve Cartridge
332
Testing the Sample Manager Backpressure Regulator
335
Replacing the Fuses
335
Cleaning the Instrument's Exterior
337
Maintaining the HT Column Heater
338
Resolving HT Column Heater Leak Sensor Errors
343
Resolving HT Column Heater Leak Sensor Errors (Door Fully Opened)
343
Replacing the HT Column Heater's Leak Sensor
347
Replacing the HT Column Heater's Leak Sensor (Door Fully Opened)
350
Replacing the Column in the HT Column Heater
352
Replacing the Column Stabilizer Assembly in the HT Column Heater
353
Replacing the Frit in the HT Column Heater's In-Line Filter Unit
354
Maintaining the Column Manager and Column Heater/Cooler
356
Resolving Column Manager and Column Heater/Cooler Leak Sensor Errors
356
Replacing the Column Manager's or Column Heater/Cooler's Leak Sensor
360
Replacing the Column in the Column Manager
362
Replacing the Column in the Column Heater/Cooler
369
Replacing the Column Stabilizer Assembly in the Column Heater/Cooler
371
Replacing the Frit in the Column Manager's In-Line Filter Unit
372
Replacing the Filter in the Column Heater/Cooler's In-Line Filter Unit
375
Cleaning the Column Manager and Column Heater/Cooler
377
Exterior Surfaces
377
Interior Surfaces
378
Maintaining the TUV Detector
379
Resolving Detector Leak Sensor Errors
380
Replacing the Detector's Leak Sensor
384
Maintaining the Flow Cell
386
Cleaning the Flow Cell
388
Performing a System Acid Cleansing Flush
391
Replacing the Flow Cell
393
Replacing the Lamp
397
Required Material
397
Replacing the Fuses
399
Cleaning the Instrument's Exterior
401
Maintaining the Sample Organizer
401
Defrosting the Sample Organizer
401
Cleaning the Air Filter
401
Replacing the Fuses
402
Cleaning the Instrument's Exterior
404
Maintaining the 30-CM Column Heater/Cooler
405
Replacing the Fuses
405
Cleaning the Instrument's Exterior
406
Troubleshooting
407
Troubleshooting with Connections Insight
408
Proper Operating Procedures
409
Contacting Waters Technical Service
409
Locating System Serial Numbers
410
Solving Baseline Noise and Drift Problems
411
Binary Solvent Manager Troubleshooting
416
Power LED
416
Flow LED
417
Sample Manager Troubleshooting
420
Power LED
420
Run LED
420
Sample Organizer Troubleshooting
423
Power LED
423
Run LED
423
HT Column Heater Troubleshooting
427
Column Manager and Column Heater/Cooler Troubleshooting
427
Power LED
427
Run LED
427
TUV Detector Troubleshooting
430
Power LED
430
Lamp LED
430
Clearing Bubbles from the Flow Cell
430
PDA Detector Troubleshooting
433
Power LED
433
Lamp LED
434
ELS Detector Troubleshooting
438
Power LED
438
Lamp LED
438
FLR Detector Troubleshooting
439
Power LED
439
Lamp LED
440
Chromatography Troubleshooting
441
A Safety Advisories
449
Warning Symbols
450
Task-Specific Hazard Warnings
450
Warnings that Apply to Particular Instruments, Instrument Components, and Sample Types
451
Biohazard Warning
452
Caution Symbol
453
Warnings that Apply to All Waters Instruments
453
Electrical and Handling Symbols
460
Electrical Symbols
460
Handling Symbols
461
B Specifications
463
Binary Solvent Manager Specifications
464
Sample Manager Specifications
466
Sample Organizer Specifications
469
HT Column Heater Specifications
473
Column Manager and Column Heater/Cooler Specifications
475
30-CM Column Heater/Cooler Specifications
477
TUV Detector Specifications
479
C Solvent Considerations
483
Introduction
484
Preventing Contamination
484
Clean Solvents
484
Solvent Quality
484
Solvent Preparation
484
Water
485
Using Buffers
485
Buffered Solvents
485
Solvent Recommendations
486
General Solvent Guidelines
486
Solvents to Use
486
Solvents to Avoid
488
ACQUITY UPLC System Recommendations
488
Binary Solvent Manager Recommendations
490
Sample Manager Recommendations
490
Detector Recommendations
490
Solvent Miscibility
490
Using Miscibility Numbers (M-Numbers
491
Solvent Stabilizers
492
Solvent Viscosity
492
Wavelength Selection
493
UV Cutoffs for Common Solvents
493
Mixed Mobile Phases
493

Waters ACQUITY UPLC Overview And Maintenance Manual (141 pages)

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Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual (124 pages)

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Copyright Notice

2
Trademarks

2
Contacting Waters

3
Customer Comments

3
Safety Considerations

3
- Considerations Specific to the ACQUITY Pda/Eλpda Detector
  4
Operating the ACQUITY Pda/Eλpda Detector

5
ISM Classification

7
EC Authorized Representative

7
- Table of Contents
  9
Overview

13
Detector Optics

13
- Calculating Absorbance
  16
Operating Principles of the Light-Guiding Flow Cell

17
Flow Cell Options

29
Before You Begin

30
Installing the Detector

31
Plumbing the Detector

33
- Installing the Multi-Detector Drip Tray
  37
Making Ethernet Connections

39
- I/O Signal Connector
  39
Connecting to the Electricity Source

40
Starting the Detector

41
- Monitoring Detector Leds
  43
- About the Detector Control Panel
  43
Shutting down the Detector

46
- Shutting down for Less than 24 Hours
  46
- Shutting down for more than 24 Hours
  46
Maintaining the Detector

48
Spectral Contrast Theory

73
- Comparing Absorbance Spectra
  73
- Representing Spectra as Vectors
  74
- Spectral Contrast Angles
  76
- Undesirable Effects
  79
Error Messages and Troubleshooting

83
Safety Advisories

92
- Warning Symbols
  92
- Caution Symbol
  95
- Warnings that Apply to All Waters Instruments
  96
- Electrical and Handling Symbols
  102
Specifications

104
- ACQUITY UPLC PDA Detector Specifications
  104
- ACQUITY UPLC Eλpda Detector Specifications
  107
Solvent Considerations

110
- Introduction
  110
- Solvent Miscibility
  112
- Wavelength Selection
  114
Mobile Phase Absorbance

117

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Waters ACQUITY UPLC Care And Use Manual (8 pages)

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Waters ACQUITY UPLC Operator's, Overview And Maintenance Manual (144 pages)

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