Protecting the Sample and Preventing It from Decoloration (Using the Shutter)
17.3
If the sample is continuously exposed to the strong light of the mercury lamp used for the epi-fluorescence attachment, it
may become damaged or decolorized. Be sure to close the shutter when suspending microscopy or when pausing
epi-fluorescence microscopy to perform microscopy with diascopic light. Be sure to get into the habit of performing this
operation.
Any of these shutters can be used to open/close the optical path as shutters for blocking the epi-fluorescence illumination
are provided in the following attachments:
• Manual/intelligent/motorized epi-fluorescence cube turret
• Manual/motorized HG precentered fiber illuminator (Intensilight)
• EPI motorized shutter
Opening and closing the shutter of a motorized unit
■ Motorized epi-fluorescence cube turret
Press the FL SHUTTER button to open/close the shutter of a
motorized epi-fluorescence cube turret. "The shutter is
closed" when the button is ON.
The FL SHUTTER button function is set to "open/close the
shutter built in the epi-fluorescence cube turret on the 1st
layer" as default. Changing the setting of the button function
allows you to use the FL SHUTTER button for
opening/closing the motorized epi-fluorescence cube turret
on the 2nd layer".
■ Motorized shutter
For Ni-E, the motorized shutter can be attached to the
epi-fluorescence side or dia-illumination side to provide a
fast opening/closing of an epi-fluorescence illumination path
or dia-illumination path.
On the epi-fluorescence side, attach the shutter between the
epi-fluorescence attachment and the HG adapter of the HG
precentered fiber illuminator. On the dia-illumination side, it
is attached between the lamphouse mount at the rear of the
microscope base and the dia-illumination lamphouse.
One motorized shutter can be attached on the
epi-fluorescence and dia-illumination side respectively for
use if two shutters are provided. This allows for immediate
blocking of the diascopic light, and is useful when switching
between epi-fluorescence and differential interference
contrast methods. (A different attachment adapter is used for
EPI and DIA sides.)
Press the FL SHUTTER button to open/close the motorized
shutter. Change the FL SHUTTER button function to the
opening/closing operation function of an EPI motorized
shutter or DIA motorized shutter. "The shutter is closed"
when the button is ON.
Chapter 3
Individual Operations
ON:
Shutter
open
73
Opening/closing the shutter
B A 4 0 0
_ _ _ _ _
D A P I
_
- 2
E X 3 3 0 - 3 8 0
_
U V - 2 B
'
Shutter of the
epi-fluorescence cube turret
on the 1st layer
Shutter of the
epi-fluorescence cube
turret on the 2nd. layer
P A _ _ 2 x _ _ _ _
Z :
_ _ _ _ _
U V - 2 B
C
_ _ _ _
N D
_
1
'
'
Shutter of the
epi-fluorescence cube
turret on the 1st layer
Shutter in the HG
precentered fiber illuminator
C: shutter closed
O: shutter open
EPI motorized shutter
DIA motorized shutter
Motorized shutter
ND
ND
NCB
8
32
11
OUT
IN
C
_ _ _ _ _
'
C
_ _
E P I : C
EPI motorized
shutter
0 . 0 0 0 u m
C
_ _
E P I : C
EPI motorized
shutter