Using Optical Elements - Nikon Eclipse Ni-E Instruction Manual

Using Optical Elements

16.2
■ DIC module for the condenser
There are three types of DIC modules to be attached to the LWD condenser: [D-C DIC N1 DRY], [D-C DIC N2 DRY]
and [D-C DIC NR DRY]. Note that an inappropriate combination may result in an inability to produce an interference
contrast image, or a significant degradation of contrast. Select a DIC module appropriate for the objective to be used
or suitable for your microscopy purpose. For details, see the table "Combinations for the DIC Slider for the Objective
and the DIC Module for the Condenser" in Chapter 1 "3. Assembly Method – 9 Attach the condenser" in the
"Assembly/Maintenance" instruction manual..
See also "9 Using the Condenser" for details.
■ DIC slider for the objective
Insert a DIC slider to a slot where the objective of the nosepiece is set.
Be sure to insert it properly to the limit.
See also "11.4 Inserting/Removing the DIC Slider" and "11.5 Single nosepiece".
■ Analyzer cube
The analyzer cube is attached to the turret section of the epi-fluorescence cube turret. There are two types of analyzer
cubes: [IR-A] for differential interference contrast microscopy in the infrared ray range and [VIS-A] for differential
interference contrast microscopy in the visible light range. Turn the turret to bring the analyzer cube to be used into the
optical path.
See also "17.1 Epi-fluorescence Cube Turret Operation".
■ Polarizer
There are two types of polarizer: polarizer for the infrared ray range and polarizer for the visible light range. Attach it to the
polarizer turret which is mounted on the field lens and turn the turret to bring the polarizer into the optical path. See also
"12 Using the Polarizer Turret".
■ FL/DIC analyzer slider
This is an optical element required for switching the
epi-fluorescence microscopy and differential interference
contrast microscopy in the visible light range or using both
microscopy methods concurrently.
Insert it into a slot on the upper lateral right of the
epi-fluorescence cube turret.
The slider has two click positions. The slider will click once
after insertion into the slot. At this point, the analyzer is not in
the optical path. To bring the analyzer into the optical path,
push the slider further to the second click-stop position.
Insert it properly to the limit.
The analyzer slider attached to the nosepiece is not required
when using the FL/DIC analyzer slider.
Chapter 3
Individual Operations
68
NI-FA FL/DIC
Analyzer Slider
2
3
4
5
6
Inserting/removing the FL/DIC analyzer slider
A. ST O
F. ST OP