Focus On The Sample - Nikon Eclipse Ni-E Instruction Manual

10

Focus on the sample.

(1) Look into the eyepiece, and adjust the brightness
appropriately by turning the dia-illumination
brightness control knob.
(2) Looking through the eyepiece, focus on the sample
by rotating in the order of the coarse focus knob and
then the fine focus knob. (See Chapter 3, "4.1
Proper Focusing Procedure".)
Z-RESET
The Z-axis coordinate value can be reset to zero.
This is useful when you wish to use the current
position as the reference position in adjusting the
focus. (See Chapter 3, "4.3 Resetting the Z-axis
Coordinate".)
Refocusing
This operation moves the Z-axis position to the
retracting position or returns it to the original
position. (See Chapter 3, "4.4 Refocusing".)
Note on controlling the focus knobs
Avoid the following action, which can cause equipment
malfunction.
• Rotating the right and left focus knobs in opposite
directions.
Chapter 2
Microscopy Operations
IR-DIC Microscopy Preparation
Procedure
1 2 3 4 5 6
Focus and Orientation Microscopy
7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
Brightness control
Brightness control
Focusing using the focus knob
P A _ 1 0 x _ _ _ _
O P E N _ _ A S 3 0 . 6 m m _ _
Do not turn the focus knobs in opposite direction!
18
A . S
Z : _ _ _ _ _ 1 . 2 3 4 u m
F S 3 0 . 0 m m
Z-axis coordinate
A . S