Epi-Fluorescence Microscopy Procedure - Nikon Eclipse Ni-E Instruction Manual

4

Epi-fluorescence Microscopy Procedure

This section describes the epi-fluorescence microscopy procedure which is performed using the microscope's controls
and buttons.
Precautions on switching between epi-fluorescence microscopy IR-DIC microscopy are also described.
Find the target in the sample with dia-illumination as described in "3 IR-DIC (Infrared Ray Differential Interference
Contrast) Microscopy Procedure" and then prepare for epi-fluorescence microscopy.
The microscope can also be controlled from DS-L3 if a DS-L3 DS Camera Control Unit is connected to the microscope.
Examples of DS-L3's [MICROSCOPE CONTROL] screen with the used control buttons are shown for procedures that can
be performed from DS-L3. The content and layout of buttons on the screens in the figure and the actual [MICROSCOPE
CONTROL] screen may differ because the setting of buttons on the [MICROSCOPE CONTROL] screen can be changed
as desired.
For details on the procedure for controlling the microscope from DS-L3, see Chapter 3, "20 Operation on DS-L3" - "20.2
Microscope Control" in this manual.
DS-L3 screens in Ni-E (focusing nosepiece system)
The motorized nosepiece, motorized condenser, etc. are not used in the Ni-E focusing nosepiece system although the
DS-L3 screens in this manual show the system in which the motorized devices are fully equipped.
The buttons for the unavailable motorized devices and the devices which are not connected to the microscope will be
grayed out and cannot be operated in the actual DS-L3 screen.
WARNING
The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling
because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at
the beginning of this instruction manual.
Preparation
Find the target in the sample with dia-illumination as described in "3 IR-DIC (Infrared Ray (Differential Interference Contrast)
Microscopy". (See Chapter 3, "17.7 Other Notes on Epi-fluorescence Microscopy".)
1
Turn off the dia-illumination lamp.
Press the dia-illumination ON/OFF switch.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
29
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
Turning off dial-illumination lamp
P F _ 1 0 x _ _ _ _ Z :
D I A : O F F _ _ _ _ _ _ _ _ _
[MICROSCOPE CONTROL] screen
[LAMP ON/OFF] button: Lamp OFF
_ _ _ _ _ 0 . 0 0 0 u m
F S 3 0 . 0 m m