Using The Dia-Illumination Section; Opening/Closing The Dia-Illumination Shutter; Using Diascopic Illumination - Nikon ECLIPSE Ti2-U Instruction Manual

3.3

Using the Dia-illumination Section

3.3.1

Opening/Closing the Dia-Illumination Shutter

The dia-illumination section has a shutter for
blocking the optical path.
 Operation:
Dia-illumination shutter
knob
Turn to the O (Open) side
Turn to the C (Close) side
Using the dia-illumination shutter
By using the dia-illumination shutter, illumination can be temporarily blocked while dia-illumination is turned
on. By closing the dia-illumination shutter during Epi-FL microscopy, the LED of the dia-illumination section
is prevented from emitting autofluorescence due to epi illumination.
3.3.2

Using Diascopic Illumination

Control dia-illumination using the dia-illumination
ON/OFF button and brightness adjuster on the left
side of the microscope.
 Operation:
Turning on/off dia-illumination
Dia-illumination
ON/OFF button
Press the button.
Adjusting dia-illumination
Brightness adjuster
Turn it clockwise.
Turn it counterclockwise.
Using the brightness adjuster
The brightness adjuster with the upper and the lower limits rotates only within the limits.
The alarm tone does not sound even though the adjuster reaches the limits. Do not forcibly turn the
adjuster.
Dia-illumination shutter
Open
Close
Dia-illumination
Pressing the button
alternates between on
and off.
Dia-illumination
Illumination becomes
lighter.
Illumination becomes
darker.
Dia-illumination
shutter knob
Opening/closing the dia-illumination shutter
Dia-illumination
brightness
adjuster
Controlling dia-illumination
33
Chapter 3 Usage of Components
C
F .
S .
Dia-illumination
ON/OFF button