Adjust The Field Diaphragm - Nikon Eclipse Ni-E Instruction Manual

17

Adjust the field diaphragm.

Press the DIA field diaphragm button to adjust the field
diaphragm so that it circumscribes the field of view.
Size of the field diaphragm
Normally, the field diaphragm should be adjusted
so that it circumscribes the field of view.
Excessively opening the field diaphragm will result
in stray light entering the field of view, generating
flare and reducing the image contrast. It will also
cause the sample to become decolorized over a
wider area.
Field diaphragm adjustment timing
Be sure to adjust the field diaphragm each time
you change the objective.
Chapter 2
Microscopy Operations
IR-DIC Microscopy Preparation
Procedure
1 2 3 4 5 6
Focus and Orientation Microscopy
7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
Adjusting field diaphragm
_ _ _ _ _ _ _ _ _ _
O P E N _ _ _ _ _ _ _ _ _ _ _ _
Field diaphragm
Circumscribe around
Right Size of the Field Diaphragm
[F. STOP] button, sub screen: DIA field diaphragm
22
ND ND NCB
8 32 11
OUT
IN
Z : _ _ _ _ _ 1 . 2 3 4 u m
F S 2 5 . 0 m m
Field of view
the field of view.
adjustment