Nikon Eclipse Ci-E Instruction Manual
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Nikon Eclipse Ci-E Instruction Manual

Nikon Eclipse Ci-E Instruction Manual

Upright motorized microscope
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M569E
17.9.NF.9 (1/3)
*M569EN09*
Upright Motorized Microscope
Instruction Manual

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  • Page 1 M569E 17.9.NF.9 (1/3) *M569EN09* Upright Motorized Microscope Instruction Manual...

  • Page 3: Introduction

    Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of the Nikon ECLIPSE Ci-E microscope. To ensure correct usage, read this manual carefully before operating this product. ● No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

  • Page 4: Contents Of The Manual

    Introduction Contents of the Manual The manual for ECLIPSE Ci-E consists of the following contents. There is also a "Bright-field Microscopy Quick Guide", provided as a separate document.  This manual: Instructions Safety Precautions Microscopy Procedures Bright-field Microscopy Phase Contrast Microscopy...

  • Page 5: Table Of Chapters

    Table of Chapters Table of Chapters (See next page for the detailed contents.) Safety Precautions/ Introduction Notes on Handling the Product Contents of the Manual Symbols Used in This Manual Chap. 1-1 Microscopy Procedures Bright-field Microscopy Chap. 1-2 Microscopy Procedures Phase Contrast Microscopy Chap.

  • Page 6: Table Of Contents

    Contents Contents Introduction ......................... i Contents of the Manual ....................ii Symbols Used in This Manual ..................ii Table of Chapters ......................iii Safety Precautions ......................vii WARNING and CAUTION Symbols ................vii Meaning of Symbols Used on the Product ..............vii WARNING .......................
  • Page 7 Photomicroscopy ....................64 18.2 Tips on Microscope Settings for Photomicroscopy .......... 65 Assembly ....................67 ECLIPSE Ci-E System Configuration ..............67 Assembly for Bright-field Microscopy ..............68 Assembly for Phase Contrast Microscopy .............. 73 Assembly for the Simple Polarizing Microscopy ............. 73 Assembly for Sensitive Polarization Microscopy .............
  • Page 8 Contents Decontaminating the Product ................88 Storage ........................88 Regular Inspections (Charged) ................88 Specifications and Safety Standards ........... 89 Microscopy (Principles) ................... 89 Performance Properties ..................89 Physical Properties ....................90...

  • Page 9: Safety Precautions

    Safety Precautions Safety Precautions To ensure correct and safe operation, read this manual before using this product. WARNING and CAUTION Symbols Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage.
  • Page 10 ● Ultraviolet light Safety is a top design priority for Nikon products. When lit, mercury lamps radiate ultraviolet light Safety is ensured as long as the user observes all that can damage the eyes and skin. Direct viewing of the light may result in blindness.
  • Page 11 Cautions on sustained observations enters, stop the use of the product and contact To relieve fatigue resulting from long observation your nearest Nikon representative. sessions, limit continuous observations to one hour. Take at least 10 to 15 minutes breaks Do not place any object on top of the product.

  • Page 12: Notes On Handling The Product

    Electromagnetic Environment ● Do not place this product in a locker or cabinet. Before using this product, Nikon recommends evaluating the electromagnetic environment of the Handling of focus knob installation site.

  • Page 13: Microscopy Procedures

    Microscopy Procedures Bright-field Microscopy System Configuration and Controls This section explains an example system configuration and the controls required for bright-field microscopy using the ECLIPSE Ci-E. Names of components are denoted in the following manner: [Eyepiece]. Chap. 1-1 Diopter adjustment ring...
  • Page 14 Chapter 1 Microscopy Procedures Nosepiece address buttons Chap. 1-1 Toggle pattern switch TOGGLE PATTERN MEMORY [Remote control pad] Setting mode switch...

  • Page 15: Bright-Field Microscopy Procedure

    Chapter 1 Microscopy Procedures Bright-field Microscopy Procedure Turn on the power. (If using the toggle function) Configure the toggle pattern. Switch to operation mode (left side). Turn ON the dia-illumination LED. Chap. 1-1 Lower condenser slightly from uppermost position. Fully open the field and aperture diaphragms.
  • Page 16 Chapter 1 Microscopy Procedures Select the setup target with the toggle pattern switch, and then switch to the setting mode to configure the motorized functions. Before using a motorized swing-out condenser for the first time, configure the swing-out of the top lens. (→See Chapter 2, Section 13.1 for details.) To use the toggle function, configure the toggle pattern.
  • Page 17 Chapter 1 Microscopy Procedures Fully open the field diaphragm and aperture diaphragm. Turn the field diaphragm dial and the aperture diaphragm lever clockwise to open them completely. Chap. 1-1 Fully opening the field and aperture diaphragms Bring the 10x objective into the optical path. Press the nosepiece forward/reverse rotation buttons, or the nosepiece address button on the remote control pad to bring the 10x objective into the optical path.
  • Page 18 Chapter 1 Microscopy Procedures Rotate the stage knob to move the stage and bring the target into the optical path. (So that the sample sealed under the cover glass will be lighted.) Chap. 1-1 POWE R Bringing the target into the optical path Focus on the specimen.
  • Page 19 Chapter 1 Microscopy Procedures Notes on controlling the focus knobs Avoid the following actions, which can cause equipment malfunctions.  Rotating the right and left focus knobs in opposite directions.  Rotating the coarse focus knob past the limit. Chap. 1-1 POWER Don't rotate the knobs in opposite directions! Adjust the diopter.
  • Page 20 Chapter 1 Microscopy Procedures Focus and center the condenser. (→See Chapter 2, “5 Focusing and Centering the Condenser” for details) Look into the eyepiece with the field diaphragm stopped down to the minimum. Focus on the field diaphragm image using the condenser focus knob, then adjust the condenser centering screws to center the diaphragm image within the field of view.
  • Page 21 Chapter 1 Microscopy Procedures Microscopy operation Select the desired objective. Press the nosepiece forward/reverse rotation buttons, or the nosepiece address button on the remote control pad to move the desired objective into the optical path. 1-100x swing-out condenser Chap. 1-1 Mounting a 1-100x swing-out condenser on the Ci-E main body when using the 1x objective may result in uneven illumination around the field of...
  • Page 22 Chapter 1 Microscopy Procedures Adjust the field diaphragm. Turn the field diaphragm dial to adjust the field diaphragm so that it almost circumscribes the field of view. Size of the field diaphragm Field Normally, adjust the field diaphragm so that it diaphragm almost circumscribes the field of view.

  • Page 23: Phase Contrast Microscopy

    Phase Contrast Microscopy System Components and Controls This section explains an example system configuration and the controls required for a phase contrast microscopy using the ECLIPSE Ci-E. Names of components are denoted in the following manner: [Phase turret condenser]. Diopter adjustment ring...
  • Page 24 Chapter 1 Microscopy Procedures Nosepiece address buttons Toggle pattern switch TOGGLE PATTERN MEMORY Chap. 1-2 [Remote control pad] Setting mode switch...

  • Page 25: Phase Contrast Microscopy Procedure

    Chapter 1 Microscopy Procedures Phase Contrast Microscopy Procedure Turn on the power. (If using the toggle function) Configure the toggle pattern. Switch to operation mode (left side). Turn ON the dia-illumination LED. Lower condenser slightly from uppermost position. Move the turret to the [A: empty] position.
  • Page 26 Chapter 1 Microscopy Procedures Set the setting mode switch to the left side (operation mode). Turn on the dia-illumination LED. Lower the condenser slightly from the uppermost position. Move the condenser turret to the [A: empty] position. Turn the condenser turret until the [A: empty] symbol comes to the front, and align the hole with the optical path.
  • Page 27 Chapter 1 Microscopy Procedures Microscopy operation (→See also: Chapter 2, “16 Tips for Phase Contrast Microscopy”) Bring the Ph annular diaphragm (Ph1) in the condenser turret into the optical path. Turn the condenser turret until the [Ph1] symbol comes to the front.
  • Page 28 Chapter 1 Microscopy Procedures Bring an arbitrary Ph objective into the optical path. Press the nosepiece forward/reverse rotation buttons on the main body, or the nosepiece address button on the remote control pad to move the desired Ph objective into the optical path. When using an oil immersion type objective, apply immersion oil between the specimen and the objective.
  • Page 29 Chapter 1 Microscopy Procedures Adjust the field diaphragm. Turn the field diaphragm dial to adjust the field diaphragm so that it almost circumscribes the field of view. Size of the field diaphragm Field Normally, adjust the field diaphragm so that it diaphragm almost circumscribes the field of view.

  • Page 30: Simple Polarizing Microscopy

    System Configuration and Controls This section explains an example system configuration and the controls required for a simple polarizing microscopy using the ECLIPSE Ci-E. Names of components are denoted in the following manner: [Polarizer unit for simple polarization]. Diopter adjustment ring...
  • Page 31 Chapter 1 Microscopy Procedures Nosepiece address buttons Toggle pattern switch TOGGLE PATTERN MEMORY [Remote control pad] Setting mode switch Chap. 1-3...

  • Page 32: Simple Polarizing Microscopy Procedure

    Chapter 1 Microscopy Procedures Simple Polarizing Microscopy Procedure Turn on the power. (If using the toggle function) Configure the toggle pattern. Switch to operation mode (left side). 9 15 Turn ON the dia-illumination LED. Lower condenser slightly from uppermost position. Fully open the field and aperture diaphragms.
  • Page 33 Chapter 1 Microscopy Procedures Set the setting mode switch to the left side (operation mode). Turn on the dia-illumination LED. Lower the condenser slightly from the uppermost position. Fully open the field diaphragm and aperture diaphragm. Bring the 10x objective into the optical path. Place a specimen on the stage, and move the stage to bring the target into view.
  • Page 34 Chapter 1 Microscopy Procedures Microscopy operation Bring a portion of the specimen where there is no sample into the optical path. Rotate the stage knob to move the specimen and bring a portion where there is no sample under the cover glass into the optical path.
  • Page 35 Chapter 1 Microscopy Procedures Adjust the orientation of the analyzer and the polarizer. Fully open the aperture diaphragm. Pull out one eyepiece from the tube. Look into the eyepiece sleeve and rotate the whole polarizer unit until you can identify a dark cross. (You will see black stripes that change shape as you rotate the polarizer unit.) Tighten the fixing screw of the polarizer unit to fix the polarizer.
  • Page 36 Chapter 1 Microscopy Procedures View the specimen. Rotate the stage knob to move the target. If the target is not in focus, use the focus knob to adjust the focus. Strict polarizing microscopy If you need a retardation measurement or stricter polarizing observation, use a dedicated polarizing microscope.

  • Page 37: Sensitive Polarization Microscopy

    System Configuration and Controls This section explains an example system configuration and the controls required for a sensitive polarization microscopy using the ECLIPSE Ci-E. Names of components are denoted in the following manner: [Polarizer unit for first-order red compensation]. Diopter adjustment ring...
  • Page 38 Chapter 1 Microscopy Procedures Nosepiece address buttons Toggle pattern switch TOGGLE PATTERN MEMORY [Remote control pad] Setting mode switch Chap. 1-4...

  • Page 39: Sensitive Polarization Microscopy Procedure

    Chapter 1 Microscopy Procedures Sensitive Polarization Microscopy Procedure Turn on the power. Configure the motorized functions. Switch to operation mode (left side). Turn ON the dia-illumination LED. Lower condenser slightly from uppermost position. Fully open the field and aperture diaphragms. Bring the 10x objective into the optical path.
  • Page 40 Chapter 1 Microscopy Procedures Set the setting mode switch to the left side (operation mode). Turn on the dia-illumination LED. Lower the condenser slightly from the uppermost position. Fully open the field diaphragm and aperture diaphragm. Bring the 10x objective into the optical path. Place a specimen on the stage, and move the stage to bring the target into view.
  • Page 41 Chapter 1 Microscopy Procedures Microscopy operation Bring a portion of the specimen where there is no sample into the optical path. Rotate the stage knob to move the specimen and bring a portion where there is no sample under the cover glass into the optical path.
  • Page 42 Chapter 1 Microscopy Procedures Adjust the orientation of the analyzer and the polarizer. Fully open the aperture diaphragm. Pull out one eyepiece from the tube. Rotate the upper portion of the polarizer unit to remove the lambda plate from the optical path. When doing this, support the polarizer unit for first-order red compensation with hands so that it does not move.
  • Page 43 Chapter 1 Microscopy Procedures View the specimen. Swing out the lambda plate from the optical path.(The field of view gets darker.) Rotate the stage knob to move the target. If the target is not in focus, use the focus knob to adjust the focus. (The specimen looks brighter in the dark field of view.) Bring the lambda plate back into the optical path.(The background of the field of view will be colored magenta.) Of the needle-like crystals seen in the field of view, check the color of the longitudinal ones.

  • Page 44: Epi-Fluorescence Microscopy

    Epi-fluorescence Microscopy System Configuration and Controls This section explains an example system configuration and the controls required for an epi-fluorescence microscopy using the ECLIPSE Ci-E. Names of components are denoted in the following manner: [CI-FL epi-fluorescence attachment]. Diopter Optical path...
  • Page 45 Chapter 1 Microscopy Procedures Nosepiece address buttons Toggle pattern switch TOGGLE PATTERN MEMORY [Remote control pad] Setting mode switch Chap. 1-5...

  • Page 46: Epi-Fluorescence Microscopy Procedure

    Chapter 1 Microscopy Procedures Epi-fluorescence Microscopy Procedure WARNING The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at the beginning of this instruction manual.
  • Page 47 Chapter 1 Microscopy Procedures Close the shutter and block the illumination path. Set the shutter open/close lever of the epi-fluorescence Filter cube switching knob attachment to position “C” to close the shutter and block the optical path. Shutter of the epi-fluorescence attachment CUBE The shutter blocks illumination.
  • Page 48 When using an oil immersion type objective, apply immersion oil between the specimen and the objective. (→Chapter 2 “14 Oil Immersion” for details) Non-fluorescent immersion oil Use Nikon designated non-fluorescent immersion oil. Focus on the specimen. Look into the eyepiece, and adjust the brightness of the field of view with the ND filter of the epi-fluorescence attachment.
  • Page 49 For fluorescence observations, press the dia-illumination LED ON/OFF switch to make the diascopic image disappear. Bright ambient lights will make it difficult to view the image. Nikon recommends keeping the room dark during fluorescence observations. To return to bright-field microscopy ●...
  • Page 50 Chapter 1 Microscopy Procedures Chap. 1-5...

  • Page 51: Individual Operations

    Individual Operations Adjusting the Brightness of a Diascopic Image The brightness of a diascopic image can be adjusted by turning the dia-illumination brightness control knob or by removing/inserting ND filters from or into the optical path. Adjustment with the Dia-illumination Brightness Control Knob Turning the dia-illumination brightness control knob to change the 0.

  • Page 52: Focusing On The Specimen (Vertical Stage Movement)

    Chapter 2 Individual Operations Focusing on the Specimen (Vertical Stage Movement) Notes on controlling the focus knobs Avoid the following actions, which can cause equipment malfunctions.  Rotating the right and left focus knobs in opposite directions.  Rotating the coarse focus knob past the limit. POWER Don't rotate the knobs in opposite directions! Turn the coarse or fine focus knob to raise or lower the stage and...

  • Page 53: Focus Knob Rotation And Stage Movement

    Chapter 2 Individual Operations Focus Knob Rotation and Stage Movement Both the coarse focus knob and the fine focus knob are located on both right and left sides on the microscope. The table below shows the relationship of the focus knobs' rotation with the stage movement.

  • Page 54: Adjusting The Rotating Torque Of The Coarse Focus Knob

    Chapter 2 Individual Operations Adjusting the Rotating Torque of the Coarse Focus Knob Adjust the rotation torque of the coarse focus knob (rotation resistance) by turning the torque adjustment knob (TORQUE) located at the base of the coarse focus knob. If the torque is set too low, the stage may descend under its own weight.

  • Page 55: Position Exchange Of The Fine Focus Knob

    Chapter 2 Individual Operations Position Exchange of the Fine Focus Knob Among left and right fine focus knobs, one is flat and the other is convex. Both fine focus knobs are attached to the coarse focus knobs using magnet, so you can detach the right and left knobs from the coarse focus knobs and swap them.

  • Page 56: Bringing The Specimen Into The Optical Path (Horizontal Stage Movement)

    Chapter 2 Individual Operations Bringing the Specimen into the Optical Path (Horizontal Stage Movement) Note on moving the stage Avoid the following actions, which can cause equipment malfunctions.  Moving the stage to the right and left by holding the top surface of the stage directly.

  • Page 57: Adjusting The Diopter

    Chapter 2 Individual Operations Adjusting the Diopter The diopter adjustment ring on an eyepiece can be adjusted to match the eyesight of your right and left eyes. A properly adjusted diopter compensates for differences in visual acuity between the right and left eyes of a person, making binocular observation easier.

  • Page 58: Focusing And Centering The Condenser

    Chapter 2 Individual Operations Focusing and Centering the Condenser Adjust the condenser position so that the light passing through the condenser forms an image at the correct position (center of the optical path) on the surface of the specimen. Follow Steps 1 through 9 in Chapter 1 “1.2 Bright-field Microscopy Procedure”...

  • Page 59: Adjusting The Aperture Diaphragm

    The scale on the condenser indicates the numerical aperture. Plan 40X The index on the aperture diaphragm lever should be aligned with 40x / 0.75 Nikon JAPAN Plan 40x the scale line that corresponds to 70 to 80% of the numerical 40x / 0.75...

  • Page 60: Selecting A Condenser

    Chapter 2 Individual Operations Selecting a Condenser Select a condenser optimal for the magnification of the objective and the microscopy procedure. Selecting the Magnification of the Objective and Condenser Condenser (: Optimum, ○: Suitable, ×: Not suitable) Slide achro Objective's Achromat Motorized Swing-out...

  • Page 61: Switching The Optical Path Of The Tube

    Chapter 2 Individual Operations Switching the Optical Path of the Tube Light Distribution With the ergonomic binocular tube or trinocular eyepiece tube, the optical path switching lever allows distribution of light to the binocular section and camera port. Optical path switching lever Switching the optical path of the tube Optical Path Switching Lever and Distribution of Light...

  • Page 62: Adjusting The Binocular Section

    Chapter 2 Individual Operations Adjusting the Binocular Section Adjusting with the Binocular Section of the Tube 10.1 The ergonomic tube makes it possible to tilt and extend the binocular section. Adjust the position of the binocular section for most comfortable viewing. Adjusting the binocular section Using the Eyelevel Riser 10.2...

  • Page 63: Switching Objectives

    Chapter 2 Individual Operations Switching Objectives Basic Operation 12.1 Upper right side: To switch the objective, press the nosepiece forward/reverse Nosepiece reverse rotation buttons on the main body, or the nosepiece address rotation button Rotate toward the button on the remote control pad. direction so that the nosepiece address is Setting mode switch...

  • Page 64: Switching Between Two Objectives (Toggle)

    Chapter 2 Individual Operations Switching between Two Objectives (Toggle) 12.2 Toggle function allows you to view the specimen while alternately switching between two objectives configured in advance. To switch between the objectives, use the nosepiece forward/reverse rotation buttons. Two pairs of objectives can be configured: toggle pattern 1 and toggle pattern 2.
  • Page 65 Chapter 2 Individual Operations Set the setting mode switch to the left side to operation MEMORY mode. Nosepiece address buttons 1 to 6 light for a few seconds to indicate that setup is complete. Setup via DS-L4 Microscope Camera Control Unit or a PC The toggle pattern can also be configured via DS-L4 Microscope Camera Control Unit or Ni Setup Tool.

  • Page 66: Configuring The Motorized Functions

    Chapter 2 Individual Operations Configuring the Motorized Functions Nosepiece The swing-out operation of the motorized swing-out condenser, Toggle address address initial brightness of the dia-illumination LED, and reverse rotation buttons lock from address 1 to 6 are configured using the remote control pad and buttons on the main body.

  • Page 67: Changing The Initial Brightness Of Dia-Illumination Led

    Chapter 2 Individual Operations Changing the Initial Brightness of Dia-illumination LED 13.2 The brightness of the dia-illumination LED is saved for each address of the nosepiece, and the brightness previously set for the address is automatically set if the nosepiece is switched. Note that the brightness is reset once the illumination is turned off, and is set to the configured initial brightness upon next use.

  • Page 68: Reverse Rotation Lock (Rotation From Address 1 To 6)

    Chapter 2 Individual Operations Reverse Rotation Lock (Rotation from Address 1 to 6) 13.3 Normally, the nosepiece rotates in the direction that is the shorter distance to the specified address. However, when the reverse rotation lock is enabled for the rotation from Address 1 to 6, a direct movement from Address 1 to the next Address 6 can be disabled.

  • Page 69: Oil Immersion

    After use, thoroughly wipe off all oil, and make sure that no oil remains on the tips of other objectives. Additionally, carefully wipe off the oil from the condenser. Use petroleum benzine to wipe off the immersion oil. For optimum results, Nikon recommends cleaning with absolute alcohol (ethyl or methyl alcohol) after cleaning with petroleum benzine.

  • Page 70: Water Immersion

    Chapter 2 Individual Operations Water Immersion An objective with a “WI” or “W” marking is a water-immersion objective. Such an objective is used with immersion water (distilled water or physiological saline) applied between the specimen and the tip of the objective. Microscopy procedures are the same as for oil-immersion objectives.
  • Page 71 Chapter 2 Individual Operations Using the GIF filter The GIF filter (green interference filter) improves the contrast when placed in the optical path. The filter should be installed on the field lens, or placed inside or on top of the filter cassette holder. Note, however, that it may cause ghosting when mounted inside the filter cassette holder.

  • Page 72: Tips For Epi-Fluorescence Microscopy

    Chapter 2 Individual Operations Tips for Epi-fluorescence Microscopy WARNING The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at the beginning of this instruction manual.

  • Page 73: Switching Excitation Methods

    Chapter 2 Individual Operations Light Reduction by Combined ND Filters of the Epi-fluorescence Attachment Brightness ND16 ○ ○ 1/16 ○ 1/32 ○ ○ 1/64 ○ ○ 1/128 ○ ○ 1/512 ○ ○ ○ (-: Removed from the optical path, ○: Placed into the optical path) Improving the S/N ratio (shielding tube) Exposure to excitation light from the Epi-fl attachment may cause the white LED to light up, resulting in a degradation of the S/N ratio.

  • Page 74: Selecting Filters

    Chapter 2 Individual Operations Filter Cube Motion Restricting Lever Functions Lever Filter cube switching position Position A Locked (Push in by (filter cubes cannot be switched) two notches) CUBE Switching between positions 1 and 2, or Position B between positions 3 and 4 only. (The position (Push in by at which the lever is pushed in determines one notch)
  • Page 75 Chapter 2 Individual Operations EX Filter Bandwidth and Fluorescent Image Narrow EX Filter Bandwidth Wide Brightness of fluorescent image Dark Bright Generation of autofluorescence High Degree of color fading High Barrier filter (BA filter) Barrier filters allow only fluorescent light emitted by the specimen to pass, blocking excitation light. This allows the fluorescent image to be viewed without excess illumination (dark background).

  • Page 76: Capturing Images

    Chapter 2 Individual Operations Capturing Images When a DS camera head or the DS-U3 DS Digital Camera Control Unit is connected to the microscope, you can use the Camera head Capture button on the base of the microscope to capture digital images with ease.

  • Page 77: Tips On Microscope Settings For Photomicroscopy

    Even if the overall magnification is the same on the monitor, the combination of objective and camera zoom can result in significant variations in exposure time. Nikon recommends increasing the magnification of the objective rather than of the zoom. (Generally, the numerical aperture of the objective increases with magnification. The larger the numerical aperture, the brighter the resulting image.)
  • Page 78 Chapter 2 Individual Operations ■ Specimen Photomicrography of faded specimen sections requires prolonged exposure time and results in poor color reproduction and low-quality images. Move the specimen to obtain images from a fresh section of the specimen previously unexposed to excitation light. For best results, use the phase contrast method to select a specimen section for photomicrography, and then switch to the fluorescent method to capture images.

  • Page 79: Assembly

    Assembly ECLIPSE Ci-E System Configuration ENG mount camera C mount camera photomicrography device TV adapter Centering eyepiece telescope Eyelevel riser Camera head Analyzer unit Binocular tube Trinocular tube Ergonomic tube DSC port Filter cube Camera trigger cable Spacer for the...

  • Page 80: Assembly For Bright-Field Microscopy

    WARNING If the indicated voltage and the supplied voltage differ, do not attempt to use the microscope. Contact your DS C nearest Nikon representative for advice. Checking the input voltage Attach the stage. Stage clamp screw Turn the coarse focus knob to remove the cushioning material from the substage section.
  • Page 81 Chapter 3 Assembly Attach the condenser. Turn the coarse focus knob until the elevating Condenser focus knob section is brought to the upper limit. Turn the condenser focus knob until the condenser holder reaches the bottom. Insert the condenser, adjust it to face the front, then secure it in place with the tool.
  • Page 82 Attaching eyepieces Mounting the spacer for the nosepiece (only when using the spacer for the nosepiece) Contact your nearest Nikon representative when the spacer for the nosepiece needs to be mounted or removed. Attach the objective. Screw the objective into the nosepiece. Select the nosepiece address so that the magnification of the objectives...
  • Page 83 Chapter 3 Assembly Connect connectors. Hook (same on the left) USB connector CAUTION Make sure that the power is turned OFF before you connect connectors shown below. Connection of the remote control pad Connect the remote control pad cable to a remote control DS C pad connector on the rear of the microscope.
  • Page 84 Chapter 3 Assembly Connect the power cord. WARNING Be sure to use the specified power cord. Use of other power cords may result in malfunction or fire. This product is classified as having Class I protection against electric shock. Make sure this product is connected to an appropriate protective earth terminal.

  • Page 85: Assembly For Phase Contrast Microscopy

    Chapter 3 Assembly Assembly for Phase Contrast Microscopy Follow the procedure described in Section 2 “Assembly for Bright-field Microscopy” to perform assembly. Note the following: ■ Attaching a condenser for phase contrast microscopy Use a condenser for the phase contrast microscopy in the step 3, “Attach the condenser.” The procedure is the same.

  • Page 86: Assembly For Sensitive Polarization Microscopy

    Chapter 3 Assembly Assembly for Sensitive Polarization Microscopy Follow the procedure described in Section 2 “Assembly for Bright-field Microscopy” to perform assembly. Note the following: ■ Attaching an analyzer tube for first-order red compensation Attach an analyzer tube for first-order red compensation to the microscope arm prior to the step 4, “Attach the tube.”...

  • Page 87: Assembly For Epi-Fluorescence Microscopy

    Chapter 3 Assembly Assembly for Epi-fluorescence Microscopy Follow the procedure described in Section 2 “Assembly for Bright-field Microscopy” to perform assembly. Note the following: (For details on attaching a D-FL Epi-fluorescence attachment, refer to the instruction manual provided with the D-FL Epi-fluorescence attachment.) ■...
  • Page 88 Chapter 3 Assembly ● Removing/attaching the internal spacer ● Place the filter cube on a work table with the Filter cube excitation filter facing up. Unscrew the ring retaining the excitation filter. (Be careful to avoid dropping the filter.) Remove the spacer inside the removed ring. Remove or reverse the spacer as appropriate for the particular filter cube type before its insertion.

  • Page 89: Attaching A Camera

    Chapter 3 Assembly Attaching a Camera ■ When attaching a camera head to the ergonomic tube Screw the camera head into the C mount on the DSC port. Camera head Remove the rear cover of the ergonomic tube and C-mount insert the DSC port.
  • Page 90 Chapter 3 Assembly Chap. 3...

  • Page 91: Troubleshooting

    If you detect problems that are not listed below or the problem still persists after measures are taken, turn off the device and contact your nearest Nikon representative. Optical System and Operation...
  • Page 92 Chapter 4 Troubleshooting Problem Cause Measure Check and clean them in accordance with “Dirt or dust does not rotate when the eyepiece is The lens and specimen are dirty or dusty. turned” of “A dirty or dusty field of view when looking into the eyepiece.”...
  • Page 93 If the objective does not have a stopper, do protection of the objective is pushed in. not pull because the tip cannot be turned. In this case, contact your nearest Nikon representative. Screw the objective all the way in. The objective lens is attached incorrectly.
  • Page 94 Chapter 4 Troubleshooting Problem Cause Measure Position the specimen in place on the stage. One side of the field of The specimen is tilted relative to the stage (→Chapter 1 “1.2 Bright-field Microscopy Procedure view (up, down, right, or surface. - 8 Place a specimen on the stage, and move the left) is not focused.

  • Page 95: Epi-Fluorescence Microscopy

    Clean it as appropriate. The objective or cover glass is dirty. Chap. 4 (→Chapter 5 “1.1 Cleaning Lenses”) Use the non-fluorescent immersion oil designated by Nikon. The immersion oil is fluorescent. The contrast of the (→Chapter 2, “17 Tips for Epi-fluorescence Microscopy”.) fluorescent image is poor.

  • Page 96: Phase Contrast Microscopy

    Chapter 4 Troubleshooting Phase Contrast Microscopy Problem Cause Measure Adjust so that they match. The Ph annular diaphragm of the condenser does not match the phase plate (→Chapter 1, “2.2 Phase Contrast Microscopy image of the objective. Procedure - 15 Center the Ph annular diaphragm”.) Put the Ph annular diaphragm with the same Ph code as the objective into the optical path.

  • Page 97: Electrical System

    Chapter 4 Troubleshooting Electrical System General ■ Power supply Problem Cause Measure Connect the cable properly. There is no power even The power cord is not connected, or is though the power switch (→Chapter 3, “2 Assembly for Bright-field connected improperly. is on.

  • Page 98: Epi-Fluorescence Microscopy

    (→Check your illuminator’s manual.) The lamp type is incorrect. out soon after it is turned Contact your nearest Nikon representative for The lamp is at end of its life. advice if a new lamp will burn out soon after replacement.

  • Page 99: Maintenance And Storage

    Maintenance and Storage Cleaning Clean and decontaminate the lenses and other parts in accordance with the following procedure. ■ Tools used for cleaning ・Blower ・Soft brush ・Soft cotton cloth, lens tissue, gauze, etc. ・Absolute alcohol (ethyl or methyl alcohol), medical alcohol ・Petroleum benzine (use only for cleaning immersion oil) CAUTION ...

  • Page 100: Cleaning Immersion Oil

     Switch off the microscope (press the switch to the “O” position) and wait for the lamphouse to cool before covering this product with a cover. Regular Inspections (Charged) To maintain the performance of this product, Nikon recommends periodic inspections (chargeable service). Contact your nearest Nikon representative for details. Chap. 5...

  • Page 101: Specifications And Safety Standards

    ■ Intended user It is intended for the medical professional and those who work on experimentations in the field of pathology and cytology. Performance Properties ■ ECLIPSE Ci-E Nikon Microscope Model ECLIPSE Ci-E Optical system Infinity-corrected CF optical system Objective...

  • Page 102: Physical Properties

    PSE approved detachable power cord set, 3 conductor grounding (3 conductor grounding Type VCTF 3 x 0.75 mm , 3 m long maximum, rated at 125 VAC minimum) ■ CI-FL Epi-fluorescence Attachment for Nikon Microscope Model CI-FL Epi-fluorescence attachment Optical system Infinity-corrected CF optical system...
  • Page 103 CE Marking  IVD Directive This equipment complies with the emission and immunity requirements of IEC/EN 61326-2-6.  Low Voltage Directive  EMC Directive CI-FL Epi-fluorescence Attachment for Nikon Microscope ■ Model CI-FL Epi-fluorescence attachment Operating conditions Temperature: 0°C to +40°C Humidity: 60% RH max.

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