Other Notes On Epi-Fluorescence Microscopy - Nikon Eclipse Ni-E Instruction Manual

■ Objectives
The excitation balancer should be used in combination with the following objectives. Use of other objectives may result
in unevenness in the field of view.
Plan apochromat
Motorized excitation filter wheel, motorized barrier filter wheel
Motorized excitation filter wheel and motorized barrier filter wheel can be attached on the epi-fluorescence side. See
the Ni-E (Focusing Stage System) instruction manual for details.

Other Notes on Epi-fluorescence Microscopy

17.7
■ Locating a target in the sample
The standard procedure for epi-fluorescence microscopy is to first locate the target under dia-illumination differential
interference contrast microscopy, and then switch to epi-fluorescence microscopy. To locate the target under
dia-illumination bright-field microscopy, you will need to note the following.
• Under dia-illumination bright-field microscopy, start with a 10x objective, and adequately stop down the condenser.
• Gradually increase the magnification. When the target becomes difficult to locate, switch to epi-fluorescence, and
use low excitation light.
• You can also use other techniques, such as using the edge of the sample to determine the approximate position of
the target.
■ Protecting from ultraviolet light (light shielding plate)
The light shielding plate is used to prevent the reflected ultraviolet light from entering the observer's eyes, which is
originally emitted through the objective, from the sample.
Chapter 3
Individual Operations
Plan fluor 40x/0.75
S fluor 40x/0.9
40x/0.95
40xH/1.3 100xH/1.3
40xH/1.3 100xH/1.3
60xH/1.4 100xH/1.4
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