Nikon Eclipse Ni-E Instruction Manual page 7

Contents
10 Adjusting the Field Diaphragm ................................................................................61
11 Operating the Nosepiece (objective operation).......................................................62
11.1 Objective Vertical Movement Lever (objective switching, dipping) .................. 62
11.2 Parfocal Correction of Objective ...................................................................... 63
11.3 Centering the Objective.................................................................................... 64
11.4 Inserting/Removing the DIC Slider................................................................... 64
11.5 Single nosepiece .............................................................................................. 64
12 Using the Polarizer Turret .......................................................................................65
13 Switching the Optical Path of the Tube ...................................................................65
14 Adjusting the Binocular Section ..............................................................................66
15 Water Immersion .....................................................................................................66
16 Differential Interference Contrast Microscopy .........................................................67
16.1 Tips for Differential Interference Contrast Microscopy..................................... 67
16.2 Using Optical Elements .................................................................................... 68
17 Epi-fluorescence Microscopy ..................................................................................69
17.1 Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)....... 69
17.2 Selecting Filters................................................................................................ 71
17.3 Protecting the Sample and Preventing It from Decoloration
(Using the Shutter) ........................................................................................... 73
17.4 Adjusting the Brightness of the Fluorescent Image
(Using ND Filters and the Aperture Diaphragm) .............................................. 75
17.5 Restricting the Illumination to the Area of the Sample Being Viewed
(Centering and Adjusting the Field Diaphragm) ............................................... 76
17.6 Changing the Waveform Characteristics of the Excitation Light
(D-FB Excitation Balancer)............................................................................... 77
17.7 Other Notes on Epi-fluorescence Microscopy.................................................. 78
18 Useful Functions .....................................................................................................79
18.1 Mode Function.................................................................................................. 79
18.2 Interlocking Function ........................................................................................ 82
19 Capturing Images....................................................................................................83
20 Operation on DS-L3 ................................................................................................87
20.1 Setting Up the Microscope ............................................................................... 87
20.2 Microscope Control ........................................................................................ 100
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