Protecting The Sample And Preventing It From Decoloration (Using The Shutter) - Nikon Eclipse Ni-E Instruction Manual

Protecting the Sample and Preventing It from Decoloration (Using the Shutter)

16.3
If the sample is continuously exposed to the strong light of the mercury lamp used for the epi-fluorescence attachment, it
may become damaged or decolorized. Be sure to close the shutter when suspending microscopy or when pausing
epi-fluorescence microscopy to perform microscopy with diascopic light. Be sure to get into the habit of performing this
operation.
Any of these shutters can be used to open/close the optical path as shutters for blocking the epi-fluorescence illumination
are provided in the following attachments:
 Manual/intelligent/motorized epi-fluorescence cube turret
 Manual/motorized HG precentered fiber illuminator (Intensilight)
 EPI motorized shutter
Opening and closing the shutter of a motorized unit
■ Motorized epi-fluorescence cube turret
Press the FL SHUTTER button to open/close the shutter of a
motorized epi-fluorescence cube turret. "The shutter is
closed" when the button is ON.
The FL SHUTTER button function is set to "open/close the
shutter built in the epi-fluorescence cube turret on the 1st
layer" as default. Changing the setting of the button function
allows you to use the FL SHUTTER button for
opening/closing the motorized epi-fluorescence cube turret
on the 2nd layer".
Chapter 3
Individual Operations
Shutter of the epi-fluorescence
cube turret on the 1st layer
122
Opening/closing the shutter
B A 4 0 0 _ _ _ _ _ D A P I _ - 2
E X 3 3 0 - 3 8 0 _ U V - 2 B
'
Shutter of the epi-fluorescence
cube turret on the 1st layer
Shutter of the epi-fluorescence
cube turret on the 2nd layer
P A _ _ 2 x _ _ _ _ Z : _ _ _ _ _
U V - 2 B C _ _ _ _ N D _ 1
' '
Shutter of the HG
precentered fiber illuminator
C: shutter closed
O: shutter open
ND ND NCB
8 32 11
OUT
IN
C _ _ _ _ _
'
C _ _ E P I : C
EPI motorized
shutter
0 . 0 0 0 u m
C _ _ E P I : C
EPI motorized
shutter