Focusing And Centering The Condenser - Nikon Eclipse Ni-E Instruction Manual

7

Focusing and Centering the Condenser

Adjust the condenser position so that the light passing through the condenser forms an image at the correct position
(center of the optical path) on the surface of the sample.
(1) Follow Steps 1 through 12 in Chapter 2, "3 IR-DIC (Infrared
Ray Differential Interference Contrast) Microscopy
Procedure" to focus on the sample using the 10x objective.
(2) Press the right side (
diaphragm button to stop down the field diaphragm to
minimum diameter.
(3) You can see the diaphragm image in the field of view as you
look into the eyepiece. Use the condenser focus knob to
adjust so that the field diaphragm image can be outlined
clearly.
(4) Adjust the condenser centering screws until the field
diaphragm image is at the center of the eyepiece field of
view.
(5) Bring the 40x or 60x objective into the optical path. Focus
on the sample using the parfocal correction knob of the
slider nosepiece.
(6) Turn the condenser focus knob to form the field diaphragm
image on the sample surface.
(7) Operate the DIA field diaphragm button to adjust the image
so that the field diaphragm image size is almost the same
as the field of view.
(8) If the center of the field diaphragm image is not centered,
turn the condenser centering screws to move the field
diaphragm image to the center of the field of view. This is
easiest if you adjust the field diaphragm aperture so that it is
slightly smaller than the eyepiece field of view.
Correct focusing of the field diaphragm image
If the outline of the field diaphragm image is reddish or bluish, you have turned the condenser focus knob too much.
When the outline is colorless, focusing is correct.
Field diaphragm image view with the 40x or 60x objective
A field diaphragm image focused with a 40x or 60x objective cannot be seen as clearly as when the one focused with a
10x objective.
Chapter 3
Individual Operations
mark side) of the DIA field
Field diaphragm
image
Eyepiece field ring
Minimizing the field diaphragm setting
P A _ _ 2 x _ _ _ _
B I N O _ _ _ _
Focusing the field diaphragm image and moving it
to the center of the field of view
JAPA
FN- S2N N
Focusing the field diaphragm image and opening
the field diaphragm wider
57
NC B
ND
ND 11
32
8
OU T
IN
(2)
DIA field diaphragm
button
Z : _ _ _ _ _ 0 . 0 0 0 u m
Z M : 1 . 0 x _ _ F S 1 . 5 m m
Field diaphragm minimized
A . S
(5)
Parfocal
correction
knob
40x or
60x
(6)
NC B
ND
ND 11
32
8
Condenser
OU T
focus knob
IN
(7)
DIA field diaphragm
button
Field diaphragm
image
Eyepiece field ring