Changing The Waveform Characteristics Of The Excitation Light (D-Fb Excitation Balancer) - Nikon Eclipse Ni-E Instruction Manual

Changing the Waveform Characteristics of the Excitation Light (D-FB Excitation Balancer)

17.6
By attaching the D-FB Excitation Balancer to the
epi-fluorescence attachment, the waveform characteristics of
the excitation light can be changed arbitrarily. The balancer is to
be used together with a dual-band filter cube.
■ Attaching and using the excitation balancer
(1) Turn off the power for the epi-fluorescence light source.
(2) Remove the aperture diaphragm unit from the
epi-fluorescence attachment.
(3) Insert the D-FB Excitation Balancer into the rear of the
aperture diaphragm unit, as shown in the figure. Be
careful not to touch the glass surface of the excitation
balancer.
(4) Attach the aperture diaphragm unit back onto the
epi-fluorescence attachment.
(5) Attach a dual-band filter cube onto the
epi-fluorescence attachment.
(6) Adjust the excitation balancer horizontally to attain the
desired excitation light.
■ Excitation balancer details
(1)
A
Transmittance
100%
50%
0%
350
The excitation balancer is configured to achieve almost 100% transmittance at all times for FITC, which generally has
low fluorescence.
Position of
optical path
Between (1) and (2) Variable (100 to 50%)
Between (2) and (3)
Chapter 3
Individual Operations
B
(2) (3)
A
B
FITC
DAPI
400 450
DAPI
(1) 100％
(2) 50%
Variable (50 to 0%)
(3) 0%
Excitation balancer
Attaching the excitation balancer
: Effective diameter on the aperture
diaphragm surface
B
A
TRITC
Texas-Red
500 550 600
FITC TRITC/Texas-Red
100%
100% Variable (0 to 50%)
100% 50%
100% Variable (50 to 100%)
100% 100%
77
Aperture diaphragm
unit
Wavelength
650 700
0%