Nikon Eclipse Ni-E Instruction Manual page 25

Chapter 2
Epi-fluorescence Microscopy Procedure Epi-fluorescence Microscopy Procedure
Find the target in the specimen with dia-illumination,
Turn off the dia-illumination lamp.
1
Close the shutter and block the illumination path.
2
Bring the filter cube into the optical path.
3
4 Fully open the field diaphragm and aperture diaphragm of the epi-fluorescence attachment
Turn on the mercury lamp.
5
Microscopy with different magnification
6
Open the shutter.
7
Focus and Optical System Adjustment Focus and Optical System Adjustment
Focus on the sample.
8
Center the field diaphragm of the epi-fluorescence attachment.
9
Adjust the field diaphragm of the epi-fluorescence attachment.
10
Adjust the brightness.
11
View the specimen.
12
Turn off the mercury lamp.
13
Turn off the power.
14
Switching from IR-DIC (infrared ray differential interference contrast microscopy
to epi-fluorescence microscopy
Performing epi-fluorescence/visible light range differential interference
contrast concurrent microscopy
Microscopy Operations
Preparation Preparation
View the specimen View the specimen
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