Nikon Eclipse Ni-E Instruction Manual page 53

• Fully open the field diaphragm and aperture
diaphragm of the epi-fluorescence attachment.
(5) Turn on the power of the HG precentered fiber
illuminator and continue from procedure 6 of the
epi-fluorescence microscopy procedure.
Performing epi-fluorescence/visible light range differential interference contrast concurrent
microscopy
By bringing the following optical elements into the optical
path, epi-fluorescence and differential interference
contrast in the visible light range can be simultaneously
observed by microscope:
Filter cube for the epi-fluorescence cube turret
FL/DIC analyzer slider for the epi-fluorescence cube turret
DIC slider on the objective side for the nosepiece
DIC module for the condenser
Polarizer VIS for the polarizer turret
• The analyzer on the FL/DIC analyzer slider enters into
the optical path by pushing the slider to the second
click-stop position. It is removed from the optical path
when the slider is pulled back to the first-click stop
position.
• If the diascopic image is not shown under
epi-fluorescence microscopy, press the
dia-illumination ON/OFF switch to turn on the lamp.
• Adjust the fluorescent image brightness with ND filters
in the epi-fluorescence attachment, and differential
interference contrast image brightness with ND filters
in the main body. During this, dia-illumination should
be sufficiently dimmed with the ND filter.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
39
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
F. ST OP
Field diaphragm
lever
Fully opening field and aperture diaphragms
Filter Cube
2
3
4
5
6
FN- S2N JAPAN
DIC slider
DIC module
Polarizer VIS
Epi-fluorescence /visible light range differential
interference contrast concurrent microscopy
Brightness adjustment of fluorescent image
Brightness adjustment of differential interference
contrast image
A. ST OP
Aperture
diaphragm
A. ST
F. STO P
FL/DIC
analyzer
slider
ND
8
OU T
IN
ND ND NCB
8 32 11
OUT
IN