Nikon Eclipse Ni-E Instruction Manual page 49

10 Adjust the field diaphragm of the
epi-fluorescence attachment.
Use the field diaphragm lever of the epi-fluorescence
attachment to adjust the field diaphragm so that it
circumscribes the field of view.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
circumscribes the field of view. Excessively
opening the field diaphragm will result in stray light
entering the field of view, generating flare and
reducing the image contrast. In addition, the
sample will become decolorized over a wider
area.
Field diaphragm adjustment timing
Be sure to adjust the field diaphragm each time
you change the objective.
11
Adjust the brightness.
Use ND filters on the epi-fluorescence attachment to
adjust image brightness.
The aperture diaphragm of the epi-fluorescence
attachment can also be used for brightness adjustment of
the image. Be sure to center the aperture diaphragm
before use. (See Chapter 3, "17.4 Adjusting the
Brightness of the Fluorescent Image (Using ND Filters
and the Aperture Diaphragm)".)
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
35
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
Right Size of the Field Diaphragm
Adjusting the field diaphragm
Brightness adjustment using the ND filter