Parfocal Correction Of Objective - Nikon Eclipse Ni-E Instruction Manual

(1) Move the vertical movement lever at the left end to the
center to raise the front objective.
(2) With the front objective raised, hold the grip while pressing
the slide click release button and pull the sliding nosepiece
to the front.
(3) Gently release the slide click release button and secure the
sliding nosepiece at the click-stop position.
(4) Slowly move the vertical movement lever at the center to
the right end to lower the rear objective.
Objective dipping
When the objective vertical movement lever is pushed beyond the right end, the rear (observation point) objective
descends 1 mm. When the objective vertical movement lever is pushed beyond the left end, the front (observation point)
objective descends 1 mm.
When you stop pressing the lever, it moves back to the right (or left) end position, and the objective moves back to the
original height.
Cautions on dipping operation
Do not perform dipping except when performing water immersion microscopy.
Doing so may damage the sample or objective.

Parfocal Correction of Objective

11.2
Cautions on parfocal correction
Before performing parfocal correction, make sure that the objective vertical movement lever is at the right or left end.
Normally, a dry objective and an immersion objective have
different focal lengths. Switching between these two objectives
causes misalignment of the focal point. In this case, adjust the
focus using the parfocal correction (focus) knobs on the sides of
the sliding nosepiece.
The parfocal correction knob on the left side is for adjusting the
front objective, and the parfocal correction knob on the right side
is for adjusting the rear objective.
Turning the knob clockwise raises the objective (focus position
rises).
Example: When a dry objective is attached at the front and
immersion objective is attached at the rear
(1) Move the objective vertical movement lever to the center.
(objective retracting position)
(2) Push the sliding nosepiece to bring the dry objective into the
optical path.
(3) Move the objective vertical movement lever to the left end.
(front objective observation position)
(4) Readjust the focus using the parfocal correction knob on the
left sides of the sliding nosepiece. Turning the knob
clockwise raises the objective (focus position rises).
Chapter 3
Individual Operations
Slide click
release button
Focusing
nosepiece
movement
lever
Parfocal correction knob
Right side: for rear
objective
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4
5
6
FN -S2 JAPA
N
N
Grip
Switching objective from front side to rear side
FN -S2 JAPA N
N
Parfocal correction knob
Left side: for front objective
Raising focus