Adjusting The Binocular Section; Water Immersion - Nikon Eclipse Ni-E Instruction Manual

Interlocked operation of optical path switching
The optical path switching of the motorized quadrocular tilting tube can be interlocked when pressing the [CAPTURE]
button to capture an image. See "18.2 Interlocking Function" for conditions for interlocking and interlocked operations.
Manual-type tube
A manual-type tube can also be used for Ni-E (focusing stage system). See the Ni-E (Focusing Stage System)
instruction manual for details on switching the optical path for each tube.
14

Adjusting the Binocular Section

With the quadrocular tilting tube, the angle of the binocular can
be changed. Adjust the position of the binocular section for most
comfortable viewing.
Adjustment range: 15° to 35°
The observation position can be adjusted also when the
ergonomic binocular tube is used. See the Ni-E (Focusing Stage
System) instruction manual.
15

Water Immersion

An objective with a "WI" or "W" marking is a water-immersion objective that can focus on the sample at an operating
distance under water. This objective is used by filling the chamber with water (distilled water or physiological saline) and
dipping the tip of the objective into the water.
When observing a position slightly deeper than the surface (about 0.2 mm, depending on the state of the specimen) of a
specimen such as a brain slice, Nikon recommends using infrared ray microscopy or a water immersion objective with a
correction ring (a Plan100xW).
Since water evaporates readily, monitor the immersion water during observation. Avoid filling the chamber with too much
water or spilling the water, since excess water will flow onto the stage and around the condenser, promoting corrosion. In
such cases, immediately wipe off the water.
Cleaning of water
After use, wipe off the water from the tip of the objective and condenser, then follow up by wiping with absolute alcohol.
If you observe water stains, apply a small amount of neutral detergent and wipe gently, then follow up with absolute
alcohol.
Cleaning the oil
Oil remaining on the oil-immersion objective or adhered to the dry-type objective will significantly degrade image
quality. After use, thoroughly wipe off all oil, and make sure that no oil remains on the tips of other objectives.
Use petroleum benzine to wipe off the immersion oil. For optimum results, Nikon recommends cleaning with absolute
alcohol (ethyl or methyl alcohol) after cleaning with petroleum benzine.
If petroleum benzine is unavailable, use methyl alcohol alone. When using just methyl alcohol, note that surfaces will
need to be wiped repeatedly to ensure complete removal of the immersion oil. (Usually, three or four times should be
sufficient to clean the lens.)
CAUTION
• Absolute alcohol used for cleaning off the immersion water and petroleum benzene used for cleaning immersion oil
are highly flammable. Be careful when handling these materials, particularly around open flames or when turning
the power switch on or off.
• Follow the instructions provided by the manufacturer when using absolute alcohol.
Chapter 3
Individual Operations
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Adjusting the Binocular Section
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