Close The Shutter And Block The Illumination Path - Nikon Eclipse Ni-E Instruction Manual

2

Close the shutter and block the illumination path.

Press the [FL SHUTTER] button and close the shutter in
the motorized fluorescence cube turret. The shutter is
closed when the shutter button is lit.
SHUTTER
The shutter blocks illumination.
If the sample is continuously exposed to the strong
light of the mercury lamp, it may become damaged or
decolorized.
Make sure you closed the shutter when you suspend
the microscopy or when pausing epi-fluorescence
microscopy to perform microscopy using diascopic
light. Be sure to get into the habit of performing this
operation.
Shutter blocking epi-fluorescence light
The shutter is built into the epi-fluorescence cube
turret and is also equipped on the HG precentered
fiber illuminator. Also, an EPI motorized shutter
can be attached to the microscope.
Epi-fluorescence illumination can be blocked by
closing one of the shutters. (See Chapter 3, "17.3
Protecting the Sample and Preventing It from
Decoloration (Using the Shutter)".)
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
30
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■
Lit
Closing shutter
P F _ _ 2 x _ _ _ _ Z :
U V - 2 B C _ _ _ _
'
C: Shutter closed
[SHUTTER FL] button: Open/Close of the
shutter in the motorized epi-fluorescence
cube turret
The display of the button is switched to
indicate the current open/close status.
Display [OPEN]: shutter open, [CLOSED]:
shutter closed
When an intelligent epi-fluorescence
cube turret is used, the [SHUTTER FL]
button is not displayed. Therefore you
cannot check the shutter open/close
status on DS-L3.
ND ND NCB
8 32 11
OUT
IN
_ _ _ _ _ 0 . 0 0 0 u m
N D _ 1 C _ _ E P I : C
'