System Shutdown; Mounting A Sample - Nikon A1+ User Manual

System Shutdown

1. Clean any oil immersion lenses used with the lens tissue
2. Close Nikon Nis-Elements
3. If the 640nm laser is turned on, maximise the 640 control software and select Off
then close the window
4. Shutdown the PC
Incubation chamber heater and CO2 supply if used
5. MCL Piezo (on shelf)
6. XY stage controller (on shelf)
7. A1 Controller
8. Prior brightfield LED, Switched off via Prior Optiscan II box
9. Lasers- Turn the key on the front of the laser bed anticlockwise to the vertical
position

Mounting a sample

1. Ensure the correct stage insert is mounted (see facility
staff if unsure)
2. Select a low magnification (10-20x) lens from the
touchpad objective menu
3. Use the joystick to move the stage if necessary.
Change the speed of the stage by twisting the joystick.
4. Before mounting the slide on the stage, turn the microscope focus
knob, if the Z value on the microscopes front LCD display doesn't
change as you turn, the lens is in the Escape position. Press the
Refocus button on the right side of the microscope body.
5.
Remember to invert your slide so that the cover glass is the closest surface to the
lens
5