Adjust The Orientation (Vibration Direction) Of The Polarizer And Analyzer - Nikon Eclipse Ni-E Instruction Manual

19

Adjust the orientation (vibration direction) of the polarizer and analyzer.

This adjustment is performed to determine the basic
performance of differential interference contrast method.
Perform it carefully.
(1) Check that the rear of the objective (40x or 60x) is in
the optical path and the condenser turret is at [O]
(empty position). If they are not at the right position,
set 40x (or 60x) and [O] (empty position)
accordingly.
(2) Check that the DIC slider is not in the objective
position in the optical path.
(3) Press the FL CUBE CW/CCW button and bring the
DIC analyzer cube IR of the epi-fluorescence cube
turret into the optical path.
(4) Turn the polarizer turret to bring the polarizer IR into
the optical path.
(5) Rotate the stage knob to move the location without
sample into the optical path.
(6) Loosen the polarizer fixing screw once, and turn the
polarizer rotation knurled ring on the right side of the
polarizer turret to adjust the field of view so that the
field of view becomes darkest (where dark cross
appears). When adjustment is complete, tighten the
fixing screw.
For experimentation with IR camera
During experiments using an IR camera, the
orientation cannot be adjusted visually. Adjust
the orientation so that the signal intensity
becomes minimum (the light passed through
the optical system becomes minimum).
Chapter 2
Microscopy Operations
IR-DIC Microscopy Preparation
Procedure
1 2 3 4 5 6
Focus and Orientation Microscopy
7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
Bringing DIC analyzer into optical path
_ _ _ _ _ _ _ _ _ _
I R - A _ _ _ _ _ _ _
[FL TURRET] button,
sub screen: [IR-A]
Polarizer
fixing screw
Polarizer turret
Bringing IR polarizer into optical path adjust
Polarizer
fixing screw
24
ND ND NCB
8 32 11
OUT
IN
Z : _ _ _ _ _ 0 . 0 0 0 u m
N D _ 1 C _ _ E P I : C
'
Polarizer rotation
knurled ring
position
Dark cross