Centering The Objective; Inserting/Removing The Dic Slider; Single Nosepiece - Nikon Eclipse Ni-E Instruction Manual

Centering the Objective

11.3
With the sliding nosepiece, you can center the objective to
minimize the misalignment of the center of the field of view on
the front and rear objectives.
Adjust the three centering screws in such a way that the center
of the field of view of the rear objective and the center of the field
of view of the front objective match. You can more correctly
adjust them by using an eyepiece with a crosshair.
When observing smaller samples by using methods such as
patch clamping, centering is recommended beforehand.

Inserting/Removing the DIC Slider

11.4
Insert the DIC slider required for differential interference
contrast microscopy in the slot of the objective mount of the
slider nosepiece. The slot appears when the objective is
lowered.
Make sure the DIC slider is stopped at a click-stop position
when inserted.

Single nosepiece

11.5
A single nosepiece is used only for the LWD16x or 25x water
immersion objective.
Insert the DIC slider required for differential interference
contrast microscopy in the slot of the objective mount of the
single nosepiece.
Make sure the DIC slider until is stopped at a click-stop position
when inserted.
Chapter 3
Individual Operations
64
Centering screws
Centering the Objective
FN -S JAP AN
2N
40x
10x
DIC slider
DIC slider
FN -S JAP AN
2N
16xI
DIC slider
Single nosepiece