Nikon Eclipse Ni-E Instruction Manual page 30

IR-DIC Microscopy
7 Move the epi-fluorescence cube turret to empty position,
and remove the DC slider (objective side) and polarizer from the optical path.
Press the FL CUBE CW/CCW button and bring the
epi-fluorescence cube turret's [OPEN] (empty position)
into the optical path.
Pressing the FL CUBE CW button turns the turret by one
address in the clockwise direction (as viewed from
above), while the CCW button turns the turret in the
counterclockwise direction.
If the DIC slider is in the objective side, pull it out and
remove it.
Turn the polarizer turret to bring the empty position into
the optical path.
Intelligent epi-fluorescence cube turret
Switch the intelligent epi-fluorescence cube turret
manually because it is not motorized. On DS-L3,
only information display is provided.
FL/DIC analyzer slider NI-FA/dummy
slider
For IR-DIC microscopy, the FL/DIC analyzer slider
inserted in the epi-fluorescence cube turret is not
used. If the analyzer slider is inserted, pull it out to
the first click-stop position and remove the
analyzer from the optical path. If a dummy slider is
inserted instead of the analyzer slider, be sure not
to insert all the way, but to pull it out by one
position.
The periphery of field of view may be dark if it is
inserted all the way in.
Chapter 2
Microscopy Operations
Preparation
Procedure
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ■
16
Focus and Orientation Microscopy
Bringing the [OPEN] position into optical path
_ _ _ _ _ _ _ _ _ _ Z :
O P E N _ _ _ _ _ _ _
Empty
2
3
4
5
position
Remove
the DIC slider
Empty
position
Removing the DIC optical element from the optical
path
[FL TURRET] button,
sub screen: [OPEN]
ND ND NCB
8 32 11
OUT
IN
_ _ _ _ _ 0 . 0 0 0 u m
N D _ 1 C _ _ E P I : C
'
A. ST
P
F. STO
6
FN- S2N JAPAN
ND
8
OU T
IN