Nikon Eclipse Ni-E Instruction Manual page 84

Using double layered epi-fluorescence cube turrets
Two epi-fluorescence cube turrets can be used by layering them on top of each other.
Attach an attachment using a laser as an illuminator on the 1st layer, which should be used for light stimulation when
using a double-decked turret. On the 2nd layer is attached an epi-fluorescence attachment used for the
epi-fluorescence microscopy. This double-decked configuration is supplied as a separate system, so this document
does not mention the description on that system.
Basically this document describes the epi-fluorescence cube turret on the 1st layer but mentions the one on the 2nd
layer as needed. The front display panel indicates "-2" on the back of the filter cube indication to indicate the
epi-fluorescence cube turret on the 2nd layer.
The switching of the second layer epi-fluorescence cube turret can be assigned to the FUNCTION button. (See "20
Operation on DS-L3" - "20.1 Setting Up the Microscope - (3-1) Ni-E microscope button" for details.)
Configuring the speed of the epi-fluorescence cube turret
Both epi-fluorescence cube turrets on the 1st and 2nd layers are configured for "high speed" operation upon shipment,
but can also be configured for "low speed" operation for use with thick dichroic mirrors. (See "20 Operation on DS-L3" -
"20.1 Setting Up the Microscope - (7-1) FL turret drive speed" for details.)
Interlocked operation of the epi-fluorescence cube turret
The switching of the motorized epi-fluorescence cube turret can be interlocked when pressing the [CAPTURE] button
to capture an image. See "18.2 Interlocking Function" for conditions for interlocking and interlocked operations.
Intelligent epi-fluorescence cube turret/manual epi-fluorescence cube turret
See the Ni-E (Focusing Stage System) instruction manual for details of the intelligent epi-fluorescence cube
turret/manual epi-fluorescence cube turret.
Chapter 3
Individual Operations
70