Nikon Eclipse Ni-E Instruction Manual page 98

Photomicroscopy
The photomicroscopy procedure is described below. Also see the instruction manual provided with the DS-U3, DS-L3, or
the microscope's setup/control software "Ni Setup Tool" instruction manual for the details including the camera settings.
Using DS-L3
You must choose at least the following information:
• Folder for data storage.
• Name of the file to be saved. (Or select "Auto".)
• File format and file size.
• Date and destination of data
(1) Adjust the illumination of the microscope correctly, and
adjust the focus onto the specimen image.
(2) Adjust the DS camera head attachment position.
For details on the camera head attachment, see Chapter 1 "3
Assembly Method - 18 Attach the camera and DS camera
control unit" in the "Assembly/Maintenance" instruction manual.
■DSC zooming port for quadrocular tube
Loosen the attachment direction fixing screw for the C mount so
that an image moves in the reverse direction when the stage is
moved right and left, adjust the camera position, and then
tighten the screw to fix it.
■DSC port for ergonomic binocular tube
See the Ni-E (Focusing Stage System) instruction manual for
details.
(3) Focus the image.
If the image viewed through the eyepiece appears to be in focus
but the image on the monitor is out of focus, make adjustment.
■DSC zooming port for quadrocular tube
Adjust the position of the internal lenses from the rear of the
zooming port by rotating the focal position adjustment screw
using a hex wrench to focus it.
■DSC port for ergonomic binocular tube
See the Ni-E (Focusing Stage System) instruction manual for
details.
Note that, in both ports, such out of focus situations may
also indicate incorrect diopter adjustment. Make sure you
have made diopter adjustments as well. (See Chapter 3 "6
Adjusting the Diopter".)
(4) Select the camera scene mode suitable for the
microscopy method.
(5) Adjust the camera's white balance.
To adjust the white balance, press the WB button while
capturing an image of a clear section of the specimen
slide. (For fluorescent photomicrography, Nikon
recommends adjusting the white balance under normal
bright-field microscopy conditions before capturing
images.)
Chapter 3
Individual Operations
Focal
position
adjustment
screw
84
Camera head
DSC zooming port camera position adjustment
Focusing the image using a zooming port
C mount
attachment
direction
fixing screw